By H. J. Bensted, W. Bulloch, L. Dudgeon, A. G. Gardner, E. D. W. Greig, D. Harvey, W. F. Harvey, T. J. Mackie, R. A. O'Brien, H. M. Perry, H. Scutze, P. Bruce White, W. J. Wilson. London, 1929. His Majesty's Stationery Office. Pp. 1–482
by A. Trevor Willis, M.D., B.S. (Melb.), Ph.D. (Leeds), M.C.Path., M.C.P.A., Reader in Microbiology, Monash University, formerly Lecturer in Bacteriology, University of Leeds. xiv + 234 pages, illustrated, second edition. Butterworth Inc., Washington. 1965. $8.50
Attempts to utilize the rat cecal scoring technique of Neal to measure the invasiveness of strains of Entamoeba histolytica gave inconsistent results when Sherman strain rats were used. Results with Wistar rats were more consistent.
Two strains of E. histolytica, one in culture for 11 years and another in culture for 4 years, were still as highly infective and invasive as were four strains recently isolated from cases of symptomatic amebiasis.
Strain F22 originally isolated from an amebic abscess of the brain showed diminished infectivity and invasiveness. Its infectivity and invasiveness were restored to a level comparable to recently isolated strains when organisms were passed rapidly through rats and culture.
Communicable Disease Center, Public Health Service, U. S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333.