Comparison of the Indirect Fluorescent Antibody Test and Methylene Blue Dye Test for Detection of Antibodies to Toxoplasma Gondii

Bryce C. WaltonU. S. Army Medical Research Unit Component, Middle American Research Unit, Box 2011, Balboa Heights, Canal Zone

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Barbara M. BenchoffU. S. Army Medical Research Unit Component, Middle American Research Unit, Box 2011, Balboa Heights, Canal Zone

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Wiley H. BrooksU. S. Army Medical Research Unit Component, Middle American Research Unit, Box 2011, Balboa Heights, Canal Zone

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Summary

A qualitative and quantitative evaluation of the indirect fluorescent antibody test for toxoplasmosis was made by comparison with methylene blue dye test results on 1,000 serum specimens. Over 95% agreement between the two tests was obtained in qualitative results and excellent agreement in titers was also noted, although the fluorescent antibody technique tended to yield higher titers. This test appears to equal the dye test in specificity and sensitivity and eliminates the need for living organisms and human serum accessory factor.

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