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Studies on Trachoma
II. Comparison of Fluorescent Antibody, Giemsa, and Egg Isolation Methods for Detection of Trachoma Virus in Human Conjunctival Scrapings
Roger L. Nichols
Roger L. Nichols
The Department of Microbiology of the Harvard School of Public Health, Medical Department, Arabian American Oil Company, Boston, Massachusetts, Saudi Arabia
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Dorothy E. McComb
Dorothy E. McComb
The Department of Microbiology of the Harvard School of Public Health, Medical Department, Arabian American Oil Company, Boston, Massachusetts, Saudi Arabia
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Nadim Haddad
Nadim Haddad
The Department of Microbiology of the Harvard School of Public Health, Medical Department, Arabian American Oil Company, Boston, Massachusetts, Saudi Arabia
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Edward S. Murray
Edward S. Murray
The Department of Microbiology of the Harvard School of Public Health, Medical Department, Arabian American Oil Company, Boston, Massachusetts, Saudi Arabia
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Summary
A controlled study of three methods—fluorescent antibody, Giemsa, and egg inoculation—for the detection of trachoma virus in human conjunctival scrapings is presented. The morphology of trachoma inclusions as shown by specific immunofluorescence is described. Evidence for the superiority of the fluorescent antibody method to Giemsa is discussed. Field and laboratory applications of this method are suggested.
Author Notes
With the technical assistance of Miss Clare Conover.
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