ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Jackson-Akers JY, Prakash V, Oliver TI, 2023. Amebic Liver Abscess. Treasure Island, FL: StatPearls Publishing.
-
2.
Tharmaratnam T, et al., 2020. Entamoeba histolytica and amoebic liver abscess in northern Sri Lanka: A public health problem. Trop Med Health 48: 2.
-
3.
Kumar R, Patel R, Priyadarshi RN, Narayan R, Maji T, Anand U, Soni JR, 2024. Amebic liver abscess: An update. World J Hepatol 16: 316.
-
4.
Weitzel T, Cabrera J, Rosas R, Noriega LM, Schiappacasse G, Vollrath V, Porte L, 2017. Enteric multiplex PCR panels: A new diagnostic tool for amoebic liver abscess? New Microbes New Infect 18: 50–53.
-
5.
Baltrušis P, Höglund J, 2023. Digital PCR: Modern solution to parasite diagnostics and population trait genetics. Parasit Vectors 16: 143.
-
6.
Lobato IM, O’Sullivan CK, 2018. Recombinase polymerase amplification: Basics, applications and recent advances. Trends Analyt Chem 98: 19–35.
-
7.
Tan M, Liao C, Liang L, Yi X, Zhou Z, Wei G, 2022. Recent advances in recombinase polymerase amplification: Principle, advantages, disadvantages and applications. Front Cell Infect Microbiol 12: 1019071.
-
8.
Lv R, Lu N, Wang J, Li Y, Qi Y, 2022. Recombinase Polymerase Amplification for Rapid Detection of Zoonotic Pathogens: An Overview. Available at: https://www.scienceopen.com/hosted-document?doi=10.15212/ZOONOSES-2022-0002. Accessed March 3, 2024.
-
9.
Clark CG, Diamond LS, 2002. Methods for cultivation of luminal parasitic protists of clinical importance. Clin Microbiol Rev 15: 329–341.
-
10.
Khairnar K, Parija SC, 2008. Detection of Entamoeba histolytica DNA in the saliva of amoebic liver abscess patients who received prior treatment with metronidazole. J Health Popul Nutr 26: 418.
-
11.
Nair G, Rebolledo M, White AC Jr., Crannell Z, Richards-Kortum RR, Pinilla AE, RamÃrez JD, López MC, Castellanos-Gonzalez A, 2015. Detection of Entamoeba histolytica by recombinase polymerase amplification. Am J Trop Med Hyg 93: 591.
-
12.
Philips SA, Manochitra K, Padukone S, Parija SC, 2018. Detection of Entamoeba Species: A comparative analysis of nested-multiplex PCR and recombinase polymerase amplification. Int J Curr Microbiol Appl Sci 7: 1803–1808.
-
13.
Khairnar K, Parija SC, Palaniappan R, 2007. Diagnosis of intestinal amoebiasis by using nested polymerase chain reaction-restriction fragment length polymorphism assay. J Gastroenterol 42: 631–640.
-
14.
Gadsby NJ, McHugh MP, Russell CD, Mark H, Morris AC, Laurenson IF, Hill AT, Templeton KE, 2015. Development of two real-time multiplex PCR assays for the detection and quantification of eight key bacterial pathogens in lower respiratory tract infections. Clin Microbiol Infect 21: 788.e1–788.e13.
-
15.
Parija SC, Mandal J, Ponnambath DK, 2014. Laboratory methods of identification of Entamoeba histolytica and its differentiation from look-alike Entamoeba spp. Trop Parasitol 4: 90–95.
-
16.
Korpe PS, Stott BR, Nazib F, Kabir M, Haque R, Herbein JF, Petri WA Jr., 2012. Evaluation of a rapid point-of-care fecal antigen detection test for Entamoeba histolytica. Am J Trop Med Hyg 86: 980–981.
-
17.
Jauset-Rubio M, et al., 2016. Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay. Sci Rep 6: 37732.
-
18.
Crannell Z, Castellanos-Gonzalez A, Nair G, Mejia R, White AC, Richards-Kortum R, 2015. Multiplexed recombinase polymerase amplification assay to detect intestinal protozoa. Anal Chem 88: 1565–1573.
-
19.
Kersting S, Rausch V, Bier FF, von Nickisch-Rosenegk M, 2014. Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis. Malar J 13: 1–9.
-
20.
Li TT, Wang JL, Zhang NZ, Li WH, Yan HB, Li L, Jia WZ, Fu BQ, 2019. Rapid and visual detection of Trichinella spp. using a lateral flow strip-based recombinase polymerase amplification (LF-RPA) assay. Front Cell Infect Microbiol 9: 1.
-
21.
Rattaprasert P, Chavananikul C, Fungfuang W, Chavalitshewinkoon-Petmitr P, Limudomporn P, 2023. Combining isothermal recombinase polymerase amplification with lateral flow assay for diagnosis of P. cynomolgi malaria infection. PLoS Negl Trop Dis 17: e0011470.
-
22.
Bharadwaj M, Bengtson M, Golverdingen M, Waling L, Dekker C, 2021. Diagnosing point-of-care diagnostics for neglected tropical diseases. PLoS Negl Trop Dis 15: e0009405.
-
23.
Portunato F, Bartalucci C, Vena A, Giacobbe DR, Porcile E, Montanelli P, Piol N, Centanaro M, Andorno E, Bassetti M, 2024. Amoebic liver abscess: Potential application of new diagnostic techniques for an old pathogen. Infect Dis Ther 13: 625–632.
-
24.
Lai MY, Hamid MA, Jelip J, Mudin RN, Lau YL, 2023. Lateral flow recombinase polymerase amplification assays for the detection of human Plasmodium species. Am J Trop Med Hyg 108: 882–886.
-
25.
Datta P, Rattan D, Sharma D, Sharma N, Kalra N, Duseja A, Angrup A, Sehgal R, 2024. Novel diagnostic approach for amoebic liver abscess using cell free (cf) DNA: A prospective study. Infect Dis 56: 259–267.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 762 | 762 | 78 |
| Full Text Views | 9 | 9 | 6 |
| PDF Downloads | 12 | 12 | 9 |
Rapid Detection of Amoebic Liver Abscess Using Recombinase Polymerase Amplification Assay in Resource-Limited Settings
Priya Datta
drpriyadatta@hotmail.com
Priya Datta
Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India;
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Divya Rattan
Divya Rattan
Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India;
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Navneet Sharma
Navneet Sharma
Department of Internal Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India;
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Naveen Kalra
Naveen Kalra
Department of Radio-Diagnosis and Imaging, Postgraduate Institute of Medical Education and Research, Chandigarh, India;
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Ajay Duseja
Ajay Duseja
Department of Hepatology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
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Sumeeta Khurana
Sumeeta Khurana
Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India;
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ABSTRACT.
Amoebic liver abscess (ALA) is a significant public health concern in tropical countries. Traditional diagnostic methods, such as microscopy, have low sensitivity, and nested polymerase chain reaction (nPCR) has lengthy turnaround times. In this study, we aimed to evaluate the effectiveness of the recombinase polymerase amplification (RPA) assay for detecting Entamoeba histolytica in amoebic liver pus samples. The assay was tested on 150 clinical pus samples collected from suspected ALA patients in the emergency department of a tertiary care center located in Chandigarh, northern India. For comparison with RPA, nPCR was also performed on these samples. Of 150 samples, 79 (53%) tested positive for E. histolytica using the RPA assay, and similar (79; 53%) number of samples tested positive with nested PCR. In addition, sequences obtained through Sanger sequencing showed high similarity with other sequences in the National Center for Biotechnology Information database and were submitted to the database. Our findings highlight the potential of RPA as a valuable tool for the accurate diagnosis of ALA.
- Supplemental Materials (PDF 254.75 KB)
Author Notes
Authors’ contributions: P. Datta: conceptualization, validation, resources, writing—review and editing, supervision, funding acquisition. D. Rattan: data curation, writing—original draft preparation, investigation, formal analysis, software. N. Sharma: conceptualization, patient enrollment, writing—review and editing. N. Kalra: provision of pus sample, data analysis, results validation. A. Duseja: patient enrollment, writing—review and editing, supervision. S. Khurana: data analysis, writing—review and editing, supervision.
Current contact information: Priya Datta, Divya Rattan, and Sumeeta Khurana, Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India, E-mails: drpriyadatta@hotmail.com, divyarattan59@gmail.com, and sumeetakhurana@hotmail.com. Navneet Sharma, Department of Internal Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India, E-mail: navneetsharma@hotmail.com. Naveen Kalra, Department of Radio-Diagnosis and Imaging, Postgraduate Institute of Medical Education and Research, Chandigarh, India, E-mail: navkal2004@yahoo.com. Ajay Duseja, Department of Hepatology, Postgraduate Institute of Medical Education and Research, Chandigarh, India, E-mail: ajayduseja@yahoo.co.in.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Jackson-Akers JY, Prakash V, Oliver TI, 2023. Amebic Liver Abscess. Treasure Island, FL: StatPearls Publishing.
-
2.
Tharmaratnam T, et al., 2020. Entamoeba histolytica and amoebic liver abscess in northern Sri Lanka: A public health problem. Trop Med Health 48: 2.
-
3.
Kumar R, Patel R, Priyadarshi RN, Narayan R, Maji T, Anand U, Soni JR, 2024. Amebic liver abscess: An update. World J Hepatol 16: 316.
-
4.
Weitzel T, Cabrera J, Rosas R, Noriega LM, Schiappacasse G, Vollrath V, Porte L, 2017. Enteric multiplex PCR panels: A new diagnostic tool for amoebic liver abscess? New Microbes New Infect 18: 50–53.
-
5.
Baltrušis P, Höglund J, 2023. Digital PCR: Modern solution to parasite diagnostics and population trait genetics. Parasit Vectors 16: 143.
-
6.
Lobato IM, O’Sullivan CK, 2018. Recombinase polymerase amplification: Basics, applications and recent advances. Trends Analyt Chem 98: 19–35.
-
7.
Tan M, Liao C, Liang L, Yi X, Zhou Z, Wei G, 2022. Recent advances in recombinase polymerase amplification: Principle, advantages, disadvantages and applications. Front Cell Infect Microbiol 12: 1019071.
-
8.
Lv R, Lu N, Wang J, Li Y, Qi Y, 2022. Recombinase Polymerase Amplification for Rapid Detection of Zoonotic Pathogens: An Overview. Available at: https://www.scienceopen.com/hosted-document?doi=10.15212/ZOONOSES-2022-0002. Accessed March 3, 2024.
-
9.
Clark CG, Diamond LS, 2002. Methods for cultivation of luminal parasitic protists of clinical importance. Clin Microbiol Rev 15: 329–341.
-
10.
Khairnar K, Parija SC, 2008. Detection of Entamoeba histolytica DNA in the saliva of amoebic liver abscess patients who received prior treatment with metronidazole. J Health Popul Nutr 26: 418.
-
11.
Nair G, Rebolledo M, White AC Jr., Crannell Z, Richards-Kortum RR, Pinilla AE, RamÃrez JD, López MC, Castellanos-Gonzalez A, 2015. Detection of Entamoeba histolytica by recombinase polymerase amplification. Am J Trop Med Hyg 93: 591.
-
12.
Philips SA, Manochitra K, Padukone S, Parija SC, 2018. Detection of Entamoeba Species: A comparative analysis of nested-multiplex PCR and recombinase polymerase amplification. Int J Curr Microbiol Appl Sci 7: 1803–1808.
-
13.
Khairnar K, Parija SC, Palaniappan R, 2007. Diagnosis of intestinal amoebiasis by using nested polymerase chain reaction-restriction fragment length polymorphism assay. J Gastroenterol 42: 631–640.
-
14.
Gadsby NJ, McHugh MP, Russell CD, Mark H, Morris AC, Laurenson IF, Hill AT, Templeton KE, 2015. Development of two real-time multiplex PCR assays for the detection and quantification of eight key bacterial pathogens in lower respiratory tract infections. Clin Microbiol Infect 21: 788.e1–788.e13.
-
15.
Parija SC, Mandal J, Ponnambath DK, 2014. Laboratory methods of identification of Entamoeba histolytica and its differentiation from look-alike Entamoeba spp. Trop Parasitol 4: 90–95.
-
16.
Korpe PS, Stott BR, Nazib F, Kabir M, Haque R, Herbein JF, Petri WA Jr., 2012. Evaluation of a rapid point-of-care fecal antigen detection test for Entamoeba histolytica. Am J Trop Med Hyg 86: 980–981.
-
17.
Jauset-Rubio M, et al., 2016. Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay. Sci Rep 6: 37732.
-
18.
Crannell Z, Castellanos-Gonzalez A, Nair G, Mejia R, White AC, Richards-Kortum R, 2015. Multiplexed recombinase polymerase amplification assay to detect intestinal protozoa. Anal Chem 88: 1565–1573.
-
19.
Kersting S, Rausch V, Bier FF, von Nickisch-Rosenegk M, 2014. Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis. Malar J 13: 1–9.
-
20.
Li TT, Wang JL, Zhang NZ, Li WH, Yan HB, Li L, Jia WZ, Fu BQ, 2019. Rapid and visual detection of Trichinella spp. using a lateral flow strip-based recombinase polymerase amplification (LF-RPA) assay. Front Cell Infect Microbiol 9: 1.
-
21.
Rattaprasert P, Chavananikul C, Fungfuang W, Chavalitshewinkoon-Petmitr P, Limudomporn P, 2023. Combining isothermal recombinase polymerase amplification with lateral flow assay for diagnosis of P. cynomolgi malaria infection. PLoS Negl Trop Dis 17: e0011470.
-
22.
Bharadwaj M, Bengtson M, Golverdingen M, Waling L, Dekker C, 2021. Diagnosing point-of-care diagnostics for neglected tropical diseases. PLoS Negl Trop Dis 15: e0009405.
-
23.
Portunato F, Bartalucci C, Vena A, Giacobbe DR, Porcile E, Montanelli P, Piol N, Centanaro M, Andorno E, Bassetti M, 2024. Amoebic liver abscess: Potential application of new diagnostic techniques for an old pathogen. Infect Dis Ther 13: 625–632.
-
24.
Lai MY, Hamid MA, Jelip J, Mudin RN, Lau YL, 2023. Lateral flow recombinase polymerase amplification assays for the detection of human Plasmodium species. Am J Trop Med Hyg 108: 882–886.
-
25.
Datta P, Rattan D, Sharma D, Sharma N, Kalra N, Duseja A, Angrup A, Sehgal R, 2024. Novel diagnostic approach for amoebic liver abscess using cell free (cf) DNA: A prospective study. Infect Dis 56: 259–267.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 762 | 762 | 78 |
| Full Text Views | 9 | 9 | 6 |
| PDF Downloads | 12 | 12 | 9 |