Chotmongkol V , Jitpimolmard S , Thavornpitak Y , 1996. Corticosteroid in tuberculous meningitis. J Med Assoc Thai 79: 83–90.
Mai NTH , Thwaites GE , 2017. Recent advances in the diagnosis and management of tuberculous meningitis. Curr Opin Infect Dis 30: 123–128.
Sullivan JL , Osborne WR , Wedgewood RJ , 1977. Adenosine deaminase activity in lymphocytes. Br J Haematol 37: 157–158.
Pormohammad A , Riahi S-M , Nasiri MJ , Fallah F , Aghazadeh M , Doustdar F , Pouriran R , 2017. Diagnostic test accuracy of adenosine deaminase for tuberculous meningitis: A systematic review and meta-analysis. J Infect 74: 545–554.
Chotmongkol V , Teerajetgul Y , Yodwut C , 2006. Cerebrospinal fluid adenosine deaminase activity for the diagnosis of tuberculous meningitis in adults. Southeast Asian J Trop Med Public Health 37: 948–952.
Raviraj , Henry RA , Rao GG , 2017. Determination and validation of a lower cut off value of cerebrospinal fluid adenosine deaminase (CSF-ADA) activity in diagnosis of tuberculous meningitis. J Clin Diagn Res 11: OC22–OC24.
Chan TCH , Chen SPL , Mak CM , Ching CK , Luk KS , Tsang YM , Leung DCW , 2020. Determination of cerebrospinal fluid adenosine deaminase activity cut-off for the diagnosis of tuberculous meningitis in Hong Kong. J Clin Pathol 73: 800–802.
Chotmongkol V , Kosallavat S , Sawanyawisuth K , Khamsai S , Kasemsap N , Vorasoot N , Kongbunkiat K , Tiamkao S , Chaimanee P , 2024. Evaluation of Seegene Anyplex MTB/NTM real-time detection assay for diagnosis of tuberculous meningitis. Orphanet J Rare Dis 19: 7.
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Tuberculous meningitis (TBM) is a common infectious disease of the central nervous system. Detection of cerebrospinal fluid (CSF) adenosine deaminase (ADA) activity for the diagnosis of TBM has a relatively high accuracy. Most previous reports determined ADA levels by following the colorimetric method of Giusti (endpoint assay). Recently, a Diazyme ADA assay kit was developed to determine CSF ADA levels. This study aimed to define the cutoff value of CSF ADA that is diagnostic for TBM with the Diazyme ADA assay kit. Adults with meningitis were included in the study. The diagnostic properties of CSF ADA for TBM as determined by the Diazyme ADA assay kit were assessed by the receiver operating characteristic curve, area under the curve, sensitivity, specificity, and likelihood ratios. There were 97 patients enrolled in the study, comprising 15 cases of TBM and 82 cases of non-TBM meningitis. The median CSF ADA activity level in the TBM group was significantly higher than in the non-TBM group (P = 0.002), with a mean difference of 14.5 U/L (95% CI: 5.3–23.8 U/L). A CSF ADA level of 6.1 U/L was the best cutoff value to differentiate between the TBM and non-TBM groups, with a sensitivity of 53.33%, a specificity of 89.02%, a positive likelihood ratio of 4.86, a negative likelihood ratio of 0.52, and an area under the curve of 0.75. A CSF ADA level of 6.1 U/L determined by the Diazyme ADA assay kit could be used as a diagnostic tool in the early diagnosis of TBM.
Disclosure: Ethical approval was provided by the Khon Kaen University Faculty of Medicine Ethics Committee as instituted by the Declaration of Helsinki (Approval #HE611173).
Current contact information: Verajit Chotmongkol, Phaosin Thongsaen, Kittisak Sawanyawisuth, Sittichai Khamsai, Narongrit Kasemsap, Nisa Vorasoot, Kannikar Kongbunkiat, and Somsak Tiamkao, Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, E-mails: vercho@kku.ac.th, phaosin@gmail.com, kittisak@kku.ac.th, sittikh@kku.ac.th, naroka@kku.ac.th, nsvrs@hotmail.com, kannikarkon@kku.ac.th, and somtia@kku.ac.th. Chanpen Sriphan and Lumyai Wonglakorn, Clinical Laboratory Section, Srinagarind Hospital, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, E-mails: chanpen@kku.ac.th and wlumya@kku.ac.th.
Chotmongkol V , Jitpimolmard S , Thavornpitak Y , 1996. Corticosteroid in tuberculous meningitis. J Med Assoc Thai 79: 83–90.
Mai NTH , Thwaites GE , 2017. Recent advances in the diagnosis and management of tuberculous meningitis. Curr Opin Infect Dis 30: 123–128.
Sullivan JL , Osborne WR , Wedgewood RJ , 1977. Adenosine deaminase activity in lymphocytes. Br J Haematol 37: 157–158.
Pormohammad A , Riahi S-M , Nasiri MJ , Fallah F , Aghazadeh M , Doustdar F , Pouriran R , 2017. Diagnostic test accuracy of adenosine deaminase for tuberculous meningitis: A systematic review and meta-analysis. J Infect 74: 545–554.
Chotmongkol V , Teerajetgul Y , Yodwut C , 2006. Cerebrospinal fluid adenosine deaminase activity for the diagnosis of tuberculous meningitis in adults. Southeast Asian J Trop Med Public Health 37: 948–952.
Raviraj , Henry RA , Rao GG , 2017. Determination and validation of a lower cut off value of cerebrospinal fluid adenosine deaminase (CSF-ADA) activity in diagnosis of tuberculous meningitis. J Clin Diagn Res 11: OC22–OC24.
Chan TCH , Chen SPL , Mak CM , Ching CK , Luk KS , Tsang YM , Leung DCW , 2020. Determination of cerebrospinal fluid adenosine deaminase activity cut-off for the diagnosis of tuberculous meningitis in Hong Kong. J Clin Pathol 73: 800–802.
Chotmongkol V , Kosallavat S , Sawanyawisuth K , Khamsai S , Kasemsap N , Vorasoot N , Kongbunkiat K , Tiamkao S , Chaimanee P , 2024. Evaluation of Seegene Anyplex MTB/NTM real-time detection assay for diagnosis of tuberculous meningitis. Orphanet J Rare Dis 19: 7.
Past two years | Past Year | Past 30 Days | |
---|---|---|---|
Abstract Views | 526 | 526 | 103 |
Full Text Views | 27 | 27 | 21 |
PDF Downloads | 33 | 33 | 28 |