ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Saijo M , 2018. Pathophysiology of severe fever with thrombocytopenia syndrome and development of specific antiviral therapy. J Infect Chemother 24: 773–781.
-
2.
Takahashi T et al., 2014. The first identification and retrospective study of severe fever with thrombocytopenia syndrome in Japan. J Infect Dis 209: 816–827.
-
3.
Kato H , Yamagishi T , Shimada T , Matsui T , Shimojima M , Saijo M , Oishi K ; SFTS Epidemiological Research Group—Japan , 2016. Epidemiological and clinical features of severe fever with thrombocytopenia syndrome in Japan, 2013–2014. PLoS One 11: e0165207.
-
4.
Casel MA , Park SJ , Choi YK , 2021. Severe fever with thrombocytopenia syndrome virus: Emerging novel phlebovirus and their control strategy. Exp Mol Med 53: 713–722.
-
5.
Oh WS et al., 2017. Effect of early plasma exchange on survival in patients with severe fever with thrombocytopenia syndrome: A multicenter study. Yonsei Med J 58: 867–871.
-
6.
Disease Control and Prevention Center and Center Hospital of the National Center for Global Health and Medicine , 2022. The 2019 REVISION of the Guidance for the Care for Patients with severe fever with Thrombocytopenia Syndrome (SFTS). Available at: http://dcc.ncgm.go.jp/information/pdf/SFTS_2019.pdf. Accessed August 4, 2023.
-
7.
Yoshikawa T et al., 2014. Sensitive and specific PCR systems for detection of both Chinese and Japanese severe fever with thrombocytopenia syndrome virus strains and prediction of patient survival based on viral load. J Clin Microbiol 52: 3325–3333.
-
8.
Demetria C et al., 2023. Evaluation of a real-time mobile PCR device (PCR 1100) for the detection of the rabies gene in field samples. Trop Med Health 51: 17.
-
9.
Kanda Y , 2013. Investigation of the freely available easy-to-use software “EZR” for medical statistics. Bone Marrow Transplant 48: 452–458.
-
10.
Shirato K , Nao N , Kawase M , Kageyama T , 2020. An ultra-rapid real-time RT-PCR method for detecting human orthopneumovirus using PCR1100. Jpn J Infect Dis 73: 465–468.
-
11.
Shirato K , Nao N , Matsuyama S , Takeda M , Kageyama T , 2021. An ultra-rapid real-time RT-PCR method using the PCR1100 to detect severe acute respiratory syndrome coronavirus-2. Jpn J Infect Dis 74: 29–34.
-
12.
Wee SK , Sivalingam SP , Yap EPH , 2020. Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocycler. Genes (Basel) 11: 1–13.
-
13.
Mehta N et al., 2019. A direct from blood/plasma reverse transcription–polymerase chain reaction for dengue virus detection in point-of-care settings. Am J Trop Med Hyg 100: 1534–1540.
-
14.
Song P et al., 2018. Deficient humoral responses and disrupted B-cell immunity are associated with fatal SFTSV infection. Nat Commun 9: 3328.
-
15.
Sun Y et al., 2012. Early diagnosis of novel SFTS bunyavirus infection by quantitative real-time RT-PCR assay. J Clin Virol 53: 48–53.
-
16.
Jalal S et al., 2021. Comparison of RT-PCR, RT-nested PCRs, and real-time PCR for diagnosis of severe fever with thrombocytopenia syndrome: A prospective study. Sci Rep 11: 16764.
-
17.
Kwon JS et al., 2018. Kinetics of viral load and cytokines in severe fever with thrombocytopenia syndrome. J Clin Virol 101: 57–62.
-
18.
Yang ZD et al., 2016. The prospective evaluation of viral loads in patients with severe fever with thrombocytopenia syndrome. J Clin Virol 78: 123–128.
-
19.
Wen HL et al., 2014. Severe fever with thrombocytopenia syndrome, Shandong Province, China, 2011. Emerg Infect Dis 20: 1–5.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 907 | 907 | 127 |
| Full Text Views | 28 | 28 | 5 |
| PDF Downloads | 30 | 30 | 8 |
Rapid Detection of Severe Fever with Thrombocytopenia Syndrome Virus in the Acute Phase of Infection by Direct Real-Time Reverse Transcription without RNA Extraction
Takehiro Hashimoto
Takehiro Hashimoto
Infection Control Center, Oita University Hospital, Oita, Japan;
Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;
Search for other papers by Takehiro Hashimoto in
Current site
Google Scholar
PubMed
Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;
Search for other papers by Takehiro Hashimoto in
Current site
Google Scholar
PubMed
Takaaki Yahiro
Takaaki Yahiro
Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;
Department of Advanced Medical Sciences, Oita University Faculty of Medicine, Oita, Japan;
Research Center for Global and Local Infectious Diseases, Oita, Japan;
Search for other papers by Takaaki Yahiro in
Current site
Google Scholar
PubMed
Department of Advanced Medical Sciences, Oita University Faculty of Medicine, Oita, Japan;
Research Center for Global and Local Infectious Diseases, Oita, Japan;
Search for other papers by Takaaki Yahiro in
Current site
Google Scholar
PubMed
Kazuma Ono
Kazuma Ono
Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;
Search for other papers by Kazuma Ono in
Current site
Google Scholar
PubMed
Search for other papers by Kazuma Ono in
Current site
Google Scholar
PubMed
Ryuichi Takenaka
Ryuichi Takenaka
Department of Emergency Medicine, Oita University Faculty of Medicine, Oita, Japan
Search for other papers by Ryuichi Takenaka in
Current site
Google Scholar
PubMed
Search for other papers by Ryuichi Takenaka in
Current site
Google Scholar
PubMed
Catalino Demetria
Catalino Demetria
Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;
Search for other papers by Catalino Demetria in
Current site
Google Scholar
PubMed
Search for other papers by Catalino Demetria in
Current site
Google Scholar
PubMed
Sakirul Khan
Sakirul Khan
Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;
Search for other papers by Sakirul Khan in
Current site
Google Scholar
PubMed
Search for other papers by Sakirul Khan in
Current site
Google Scholar
PubMed
Kazunori Kimitsuki
Kazunori Kimitsuki
Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;
Search for other papers by Kazunori Kimitsuki in
Current site
Google Scholar
PubMed
Search for other papers by Kazunori Kimitsuki in
Current site
Google Scholar
PubMed
Ryuzo Abe
Ryuzo Abe
Department of Emergency Medicine, Oita University Faculty of Medicine, Oita, Japan
Search for other papers by Ryuzo Abe in
Current site
Google Scholar
PubMed
Search for other papers by Ryuzo Abe in
Current site
Google Scholar
PubMed
Kazufumi Hiramatsu
Kazufumi Hiramatsu
Infection Control Center, Oita University Hospital, Oita, Japan;
Research Center for Global and Local Infectious Diseases, Oita, Japan;
Search for other papers by Kazufumi Hiramatsu in
Current site
Google Scholar
PubMed
Research Center for Global and Local Infectious Diseases, Oita, Japan;
Search for other papers by Kazufumi Hiramatsu in
Current site
Google Scholar
PubMed
Akira Nishizono
Akira Nishizono
Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan;
Research Center for Global and Local Infectious Diseases, Oita, Japan;
Search for other papers by Akira Nishizono in
Current site
Google Scholar
PubMed
Research Center for Global and Local Infectious Diseases, Oita, Japan;
Search for other papers by Akira Nishizono in
Current site
Google Scholar
PubMed
ABSTRACT.
No specific treatment has been developed for severe fever with thrombocytopenia syndrome (SFTS). However, the prognosis can improve with early plasma exchange. Therefore, rapid and accurate detection of SFTS virus is important for diagnosis and prognosis. Direct real-time reverse transcription polymerase chain reaction (RT-PCR) testing is easier and more time-efficient than conventional real-time RT-PCR. Our study compared direct real-time RT-PCR efficiency without the RNA extraction and purification of conventional real-time RT-PCR. Samples were collected from 18 patients with SFTS and five without SFTS. A strong correlation (r = 0.774, 95% CI: 0.652–0.857, P <0.01) was found between conventional and direct real-time RT-PCR assays. Direct real-time RT-PCR showed 84.4% sensitivity and 92.0% specificity for viral detection. Direct real-time RT-PCR is an effective diagnostic tool for patients with acute phase SFTS, but further optimization is required for viral detection.
Author Notes
Financial support: This work is supported in part by the grants from the
Current contact information: Takehiro Hashimoto, Infection Control Center, Oita University Hospital, Oita, Japan, and Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan, E-mail: hashimo2013@oita-u.ac.jp. Takaaki Yahiro, Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan, Department of Advanced Medical Sciences, Oita University Faculty of Medicine, Oita, Japan, and Research Center for Global and Local Infectious Diseases, Oita, Japan, E-mail: takaaki-816@oita-u.ac.jp. Kazuma Ono, Catalino Demetria, Sakirul Khan, and Kazunori Kimitsuki, Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan, E-mails: m1841018@oita-u.ac.jp, c_demetria@yahoo.com.ph, and sakirul@oita-u.ac.jp, k-kimitsuki@oita-u.ac.jp. Ryuichi Takenaka and Ryuzo Abe, Department of Emergency Medicine, Oita University Faculty of Medicine, Oita, Japan, E-mails: ryuichi@oita-u.ac.jp and ryuzoabe@oita-u.ac.jp. Kazufumi Hiramatsu, Infection Control Center, Oita University Hospital, Oita, Japan, and Research Center for Global and Local Infectious Diseases, Oita, Japan, E-mail: hiramats@oita-u.ac.jp. Akira Nishizono, Department of Microbiology, Oita University Faculty of Medicine, Oita, Japan and Research Center for Global and Local Infectious Diseases, Oita, Japan, E-mail: a24zono@oita-u.ac.jp.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Saijo M , 2018. Pathophysiology of severe fever with thrombocytopenia syndrome and development of specific antiviral therapy. J Infect Chemother 24: 773–781.
-
2.
Takahashi T et al., 2014. The first identification and retrospective study of severe fever with thrombocytopenia syndrome in Japan. J Infect Dis 209: 816–827.
-
3.
Kato H , Yamagishi T , Shimada T , Matsui T , Shimojima M , Saijo M , Oishi K ; SFTS Epidemiological Research Group—Japan , 2016. Epidemiological and clinical features of severe fever with thrombocytopenia syndrome in Japan, 2013–2014. PLoS One 11: e0165207.
-
4.
Casel MA , Park SJ , Choi YK , 2021. Severe fever with thrombocytopenia syndrome virus: Emerging novel phlebovirus and their control strategy. Exp Mol Med 53: 713–722.
-
5.
Oh WS et al., 2017. Effect of early plasma exchange on survival in patients with severe fever with thrombocytopenia syndrome: A multicenter study. Yonsei Med J 58: 867–871.
-
6.
Disease Control and Prevention Center and Center Hospital of the National Center for Global Health and Medicine , 2022. The 2019 REVISION of the Guidance for the Care for Patients with severe fever with Thrombocytopenia Syndrome (SFTS). Available at: http://dcc.ncgm.go.jp/information/pdf/SFTS_2019.pdf. Accessed August 4, 2023.
-
7.
Yoshikawa T et al., 2014. Sensitive and specific PCR systems for detection of both Chinese and Japanese severe fever with thrombocytopenia syndrome virus strains and prediction of patient survival based on viral load. J Clin Microbiol 52: 3325–3333.
-
8.
Demetria C et al., 2023. Evaluation of a real-time mobile PCR device (PCR 1100) for the detection of the rabies gene in field samples. Trop Med Health 51: 17.
-
9.
Kanda Y , 2013. Investigation of the freely available easy-to-use software “EZR” for medical statistics. Bone Marrow Transplant 48: 452–458.
-
10.
Shirato K , Nao N , Kawase M , Kageyama T , 2020. An ultra-rapid real-time RT-PCR method for detecting human orthopneumovirus using PCR1100. Jpn J Infect Dis 73: 465–468.
-
11.
Shirato K , Nao N , Matsuyama S , Takeda M , Kageyama T , 2021. An ultra-rapid real-time RT-PCR method using the PCR1100 to detect severe acute respiratory syndrome coronavirus-2. Jpn J Infect Dis 74: 29–34.
-
12.
Wee SK , Sivalingam SP , Yap EPH , 2020. Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocycler. Genes (Basel) 11: 1–13.
-
13.
Mehta N et al., 2019. A direct from blood/plasma reverse transcription–polymerase chain reaction for dengue virus detection in point-of-care settings. Am J Trop Med Hyg 100: 1534–1540.
-
14.
Song P et al., 2018. Deficient humoral responses and disrupted B-cell immunity are associated with fatal SFTSV infection. Nat Commun 9: 3328.
-
15.
Sun Y et al., 2012. Early diagnosis of novel SFTS bunyavirus infection by quantitative real-time RT-PCR assay. J Clin Virol 53: 48–53.
-
16.
Jalal S et al., 2021. Comparison of RT-PCR, RT-nested PCRs, and real-time PCR for diagnosis of severe fever with thrombocytopenia syndrome: A prospective study. Sci Rep 11: 16764.
-
17.
Kwon JS et al., 2018. Kinetics of viral load and cytokines in severe fever with thrombocytopenia syndrome. J Clin Virol 101: 57–62.
-
18.
Yang ZD et al., 2016. The prospective evaluation of viral loads in patients with severe fever with thrombocytopenia syndrome. J Clin Virol 78: 123–128.
-
19.
Wen HL et al., 2014. Severe fever with thrombocytopenia syndrome, Shandong Province, China, 2011. Emerg Infect Dis 20: 1–5.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 907 | 907 | 127 |
| Full Text Views | 28 | 28 | 5 |
| PDF Downloads | 30 | 30 | 8 |