ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
World Health Organization , 2022. World Malaria Report 2022. Available at: https://www.who.int/teams/global-malaria-programme/reports/world-malaria-report-2022. Accessed October 16, 2023.
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2.
Department of Disease Control of Thailand , 2023. Thailand Malaria Elimination Program. Available at: http://malaria.ddc.moph.go.th/malariar10/index_newversion.php. Accessed May 23, 2023.
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3.
Coleman RE et al., 2006. Comparison of PCR and microscopy for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand. Malar J 5: 121.
-
4.
Ochola LB , Vounatsou P , Smith T , Mabaso ML , Newton CR , 2006. The reliability of diagnostic techniques in the diagnosis and management of malaria in the absence of a gold standard. Lancet Infect Dis 6: 582–588.
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5.
Okell LC , Bousema T , Griffin JT , Ouédraogo AL , Ghani AC , Drakeley CJ , 2012. Factors determining the occurrence of submicroscopic malaria infections and their relevance for control. Nat Commun 3: 1237.
-
6.
Fitri LE , Widaningrum T , Endharti AT , Prabowo MH , Winaris N , Nugraha RYB , 2022. Malaria diagnostic update: from conventional to advanced method. J Clin Lab Anal 36: e24314.
-
7.
Ohrt C , Purnomo , Sutamihardja MA , Tang D , Kain KC , 2002. Impact of microscopy error on estimates of protective efficacy in malaria‐prevention trials. J Infect Dis 186: 540–546.
-
8.
Anthony C , Mahmud R , Lau YL , Syedomar SF , Sri La Sri Ponnampalavanar S , 2013. Comparison of two nested PCR methods for the detection of human malaria. Trop Biomed 30: 459–466.
-
9.
Ataei S , Nateghpour M , Hajjaran H , Edrissian GH , Foroushani AR , 2011. High specificity of semi-nested multiplex PCR using dried blood spots on DNA Banking Card in comparison with frozen liquid blood for detection of Plasmodium falciparum and Plasmodium vivax. J Clin Lab Anal 25: 185–190.
-
10.
Li P et al., 2014. Nested PCR detection of malaria directly using blood filter paper samples from epidemiological surveys. Malar J 13: 175.
-
11.
Canier L et al., 2015. Malaria PCR detection in Cambodian low-transmission settings: dried blood spots versus venous blood samples. Am J Trop Med Hyg 92: 573–577.
-
12.
Holzschuh A , Koepfli C , 2022. Tenfold difference in DNA recovery rate: systematic comparison of whole blood vs. dried blood spot sample collection for malaria molecular surveillance. Malar J 21: 88.
-
13.
St. Julien KR , Jelliffe-Pawlowski LL , Shaw GM , Stevenson DK , O’Brodovich HM , Krasnow MA , 2013. High quality genome-wide genotyping from archived dried blood spots without DNA amplification. PLoS One 8: e64710.
-
14.
Strøm GEA , Tellevik MG , Hanevik K , Langeland N , Blomberg B , 2014. Comparison of four methods for extracting DNA from dried blood on filter paper for PCR targeting the mitochondrial Plasmodium genome. Trans R Soc Trop Med Hyg 108: 488–494.
-
15.
Port JR , Nguetse C , Adukpo S , Velavan TP , 2014. A reliable and rapid method for molecular detection of malarial parasites using microwave irradiation and loop mediated isothermal amplification. Malar J 13: 454.
-
16.
Hsiang MS , Lin M , Dokomajilar C , Kemere J , Pilcher CD , Dorsey G , Greenhouse B , 2010. PCR-based pooling of dried blood spots for detection of malaria parasites: optimization and application to a cohort of Ugandan children. J Clin Microbiol 48: 3539–3543.
-
17.
Adeel B , Mudassar S , Kiran N , Javaid Q-u-A , Ansari F , Kausar H , Waqqar S , 2019. Prevalence of Plasmodium vivax associated malaria and screening of positive isolates for pvmdr-1 gene. RMJ 44: 57–60.
-
18.
Vaisusuk K , Chatan W , Seerintra T , Piratae S , 2022. High prevalence of Plasmodium infection in fighting cocks in Thailand determined with a molecular method. J Vet Res (Pulawy) 66: 373–379.
-
19.
Chomczynski P , Rymaszewski M , 2006. Alkaline polyethylene glycol-based method for direct PCR from bacteria, eukaryotic tissue samples, and whole blood. Biotechniques 40: 454–458.
-
20.
García-Romero N et al., 2019. Polyethylene glycol improves current methods for circulating extracellular vesicle-derived DNA isolation. J Transl Med 17: 75.
-
21.
Seesui K , Imtawil K , Chanetmahun P , Laummaunwai P , Boonmars T , 2018. An alternative method for extracting Plasmodium DNA from EDTA whole blood for malaria diagnosis. Korean J Parasitol 56: 25–32.
-
22.
Rubio JM , Post RJ , van Leeuwen WMD , Henry M-C , Lindergard G , Hommel M , 2002. Alternative polymerase chain reaction method to identify Plasmodium species in human blood samples: the semi-nested multiplex malaria PCR (SnM-PCR). Trans R Soc Trop Med Hyg 96: S199–S204.
-
23.
Hassan R , Husin A , Sulong S , Yusoff S , Johan MF , Yahaya BH , Ang CY , Ghazali S , Cheong SK , 2015. Guidelines for nucleic acid detection and analysis in hematological disorders. Malays J Pathol 37: 165–173.
-
24.
Chen H , Rangasamy M , Tan SY , Wang H , Siegfried BD , 2010. Evaluation of five methods for total DNA extraction from western corn rootworm beetles. PLoS One 5: e11963.
-
25.
Carey SJ , Becklund LE , Fabre PP , Schenk JJ , 2023. Optimizing the lysis step in CTAB DNA extractions of silica-dried and herbarium leaf tissues. Appl Plant Sci 11: e11522.
-
26.
Tomasek O , Kubicek O , Tukac V , 2008. Comparison of three template preparation methods for routine detection of beak and feather disease virus and avian polyomavirus with single and nested polymerase chain reaction in clinical specimens. Avian Pathol 37: 145–149.
-
27.
Vivantis , 2023. GF-1 Blood DNA Extraction Kit. Available at: https://www.vivantechnolo-gies.com/images/Resources/roomtempmanual/gf%201%20blood%20dna%20extraction%20kit.pdf. Accessed July 12, 2023.
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28.
Molecular Research Center , 2015. DNAzol®Direct. Available at: https://www.mrcgene.com/wp-content/uploads/2014/06/DNAzolDirectApril2015.pdf. Accessed January 14, 2023.
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29.
Miguel RB , Coura JR , Samudio F , Suárez-Mutis MC , 2013. Evaluation of three different DNA extraction methods from blood samples collected in dried filter paper in Plasmodium sub-patent infections from the Amazon region in Brazil. Rev Inst Med Trop São Paulo 55: 205–208.
-
30.
Singhanetra-Renard A , 1993. Malaria and mobility in Thailand. Soc Sci Med 37: 1147–1154.
-
31.
World Health Organization , 2010. Malaria in the Greater Mekong Subregion: Regional and Country Profiles. Available at: https://iris.who.int/handle/10665/205432. Accessed October 28, 2023.
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Parker DM et al., 2015. Microgeography and molecular epidemiology of malaria at the Thailand-Myanmar border in the malaria pre-elimination phase. Malar J 14: 198.
-
33.
Sattabongkot J , Suansomjit C , Nguitragool W , Sirichaisinthop J , Warit S , Tiensuwan M , Buates S , 2018. Prevalence of asymptomatic Plasmodium infections with sub-microscopic parasite densities in the northwestern border of Thailand: a potential threat to malaria elimination. Malar J 17: 329.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1372 | 1066 | 66 |
| Full Text Views | 457 | 76 | 6 |
| PDF Downloads | 308 | 99 | 6 |
Comparison of Three Methods to Extract Plasmodium falciparum DNA from Whole Blood and Dried Blood Spots
Thaksaporn Thongseesuksai
Thaksaporn Thongseesuksai
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
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Thidarut Boonmars
Thidarut Boonmars
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Neglected Zoonosis and Vector-Borne Disease Research Group, Khon Kaen University, Khon Kaen, Thailand
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Neglected Zoonosis and Vector-Borne Disease Research Group, Khon Kaen University, Khon Kaen, Thailand
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Porntip Laummaunwai
Porntip Laummaunwai
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Neglected Zoonosis and Vector-Borne Disease Research Group, Khon Kaen University, Khon Kaen, Thailand
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Neglected Zoonosis and Vector-Borne Disease Research Group, Khon Kaen University, Khon Kaen, Thailand
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ABSTRACT.
This study aimed to compare the effectiveness of three DNA extraction methods: the GF-1 Blood DNA Extraction Kit (GF-1 BD Kit), which employs a spin column along with lysing and washing buffers; the tris-ethylenediaminetetraacetic acid and proteinase K (TE-pK) method, which utilizes a combination of TE buffer and proteinase K for cell lysis; and DNAzol® Direct (DN 131), a single reagent combined with heating for the extraction process. Plasmodium falciparum DNA was extracted from both whole blood and dried blook spots (DBSs), with consideration of DNA concentration, purity, cost, time requirement, and limit of parasite detection (LOD) for each method. The target gene in this study was 18S rRNA, resulting in a 395-bp product using specific primers. In the comparative analysis, the DN 131 method yielded significantly higher DNA quantities from whole blood and DBSs than the GF-1 BD Kit and TE-pK methods. In addition, the DNA purity obtained from whole blood and DBSs using the GF-1 BD Kit significantly exceeded that obtained using the TE-pK and DN 131 methods. For LOD, the whole blood extracted using the DN 131, GF-1 BD Kit, and TE-pK methods revealed 0.012, 0.012, and 1.6 parasites/µL, respectively. In the case of DBSs, the LODs for the DN 131, GF-1 BD Kit, and TE-pK methods were 1.6, 8, and 200 parasites/µL, respectively. The results revealed that the TE-pK method was the most cost-effective, whereas the DN 131 method showed the simplest protocol. These findings offer alternative approaches for extracting Plasmodium DNA that are particularly well-suited for large-scale studies conducted in resource-limited settings.
Author Notes
Financial support: This study was supported in part by grants from the following organizations:
Disclosure: Ethical approval for the study was obtained from the Ethics Committee for Human Research, Khon Kaen University, Thailand (HE651337).
Authors’ addresses: Thaksaporn Thongseesuksai, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, E-mail: thaksaporn.t@kkumail.com. Thidarut Boonmars and Porntip Laummaunwai, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, and Neglected Zoonosis and Vector-Borne Disease Research Group, Khon Kaen University, Khon Kaen, Thailand, E-mails: bthida@kku.ac.th and porlau@kku.ac.th.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
World Health Organization , 2022. World Malaria Report 2022. Available at: https://www.who.int/teams/global-malaria-programme/reports/world-malaria-report-2022. Accessed October 16, 2023.
-
2.
Department of Disease Control of Thailand , 2023. Thailand Malaria Elimination Program. Available at: http://malaria.ddc.moph.go.th/malariar10/index_newversion.php. Accessed May 23, 2023.
-
3.
Coleman RE et al., 2006. Comparison of PCR and microscopy for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand. Malar J 5: 121.
-
4.
Ochola LB , Vounatsou P , Smith T , Mabaso ML , Newton CR , 2006. The reliability of diagnostic techniques in the diagnosis and management of malaria in the absence of a gold standard. Lancet Infect Dis 6: 582–588.
-
5.
Okell LC , Bousema T , Griffin JT , Ouédraogo AL , Ghani AC , Drakeley CJ , 2012. Factors determining the occurrence of submicroscopic malaria infections and their relevance for control. Nat Commun 3: 1237.
-
6.
Fitri LE , Widaningrum T , Endharti AT , Prabowo MH , Winaris N , Nugraha RYB , 2022. Malaria diagnostic update: from conventional to advanced method. J Clin Lab Anal 36: e24314.
-
7.
Ohrt C , Purnomo , Sutamihardja MA , Tang D , Kain KC , 2002. Impact of microscopy error on estimates of protective efficacy in malaria‐prevention trials. J Infect Dis 186: 540–546.
-
8.
Anthony C , Mahmud R , Lau YL , Syedomar SF , Sri La Sri Ponnampalavanar S , 2013. Comparison of two nested PCR methods for the detection of human malaria. Trop Biomed 30: 459–466.
-
9.
Ataei S , Nateghpour M , Hajjaran H , Edrissian GH , Foroushani AR , 2011. High specificity of semi-nested multiplex PCR using dried blood spots on DNA Banking Card in comparison with frozen liquid blood for detection of Plasmodium falciparum and Plasmodium vivax. J Clin Lab Anal 25: 185–190.
-
10.
Li P et al., 2014. Nested PCR detection of malaria directly using blood filter paper samples from epidemiological surveys. Malar J 13: 175.
-
11.
Canier L et al., 2015. Malaria PCR detection in Cambodian low-transmission settings: dried blood spots versus venous blood samples. Am J Trop Med Hyg 92: 573–577.
-
12.
Holzschuh A , Koepfli C , 2022. Tenfold difference in DNA recovery rate: systematic comparison of whole blood vs. dried blood spot sample collection for malaria molecular surveillance. Malar J 21: 88.
-
13.
St. Julien KR , Jelliffe-Pawlowski LL , Shaw GM , Stevenson DK , O’Brodovich HM , Krasnow MA , 2013. High quality genome-wide genotyping from archived dried blood spots without DNA amplification. PLoS One 8: e64710.
-
14.
Strøm GEA , Tellevik MG , Hanevik K , Langeland N , Blomberg B , 2014. Comparison of four methods for extracting DNA from dried blood on filter paper for PCR targeting the mitochondrial Plasmodium genome. Trans R Soc Trop Med Hyg 108: 488–494.
-
15.
Port JR , Nguetse C , Adukpo S , Velavan TP , 2014. A reliable and rapid method for molecular detection of malarial parasites using microwave irradiation and loop mediated isothermal amplification. Malar J 13: 454.
-
16.
Hsiang MS , Lin M , Dokomajilar C , Kemere J , Pilcher CD , Dorsey G , Greenhouse B , 2010. PCR-based pooling of dried blood spots for detection of malaria parasites: optimization and application to a cohort of Ugandan children. J Clin Microbiol 48: 3539–3543.
-
17.
Adeel B , Mudassar S , Kiran N , Javaid Q-u-A , Ansari F , Kausar H , Waqqar S , 2019. Prevalence of Plasmodium vivax associated malaria and screening of positive isolates for pvmdr-1 gene. RMJ 44: 57–60.
-
18.
Vaisusuk K , Chatan W , Seerintra T , Piratae S , 2022. High prevalence of Plasmodium infection in fighting cocks in Thailand determined with a molecular method. J Vet Res (Pulawy) 66: 373–379.
-
19.
Chomczynski P , Rymaszewski M , 2006. Alkaline polyethylene glycol-based method for direct PCR from bacteria, eukaryotic tissue samples, and whole blood. Biotechniques 40: 454–458.
-
20.
García-Romero N et al., 2019. Polyethylene glycol improves current methods for circulating extracellular vesicle-derived DNA isolation. J Transl Med 17: 75.
-
21.
Seesui K , Imtawil K , Chanetmahun P , Laummaunwai P , Boonmars T , 2018. An alternative method for extracting Plasmodium DNA from EDTA whole blood for malaria diagnosis. Korean J Parasitol 56: 25–32.
-
22.
Rubio JM , Post RJ , van Leeuwen WMD , Henry M-C , Lindergard G , Hommel M , 2002. Alternative polymerase chain reaction method to identify Plasmodium species in human blood samples: the semi-nested multiplex malaria PCR (SnM-PCR). Trans R Soc Trop Med Hyg 96: S199–S204.
-
23.
Hassan R , Husin A , Sulong S , Yusoff S , Johan MF , Yahaya BH , Ang CY , Ghazali S , Cheong SK , 2015. Guidelines for nucleic acid detection and analysis in hematological disorders. Malays J Pathol 37: 165–173.
-
24.
Chen H , Rangasamy M , Tan SY , Wang H , Siegfried BD , 2010. Evaluation of five methods for total DNA extraction from western corn rootworm beetles. PLoS One 5: e11963.
-
25.
Carey SJ , Becklund LE , Fabre PP , Schenk JJ , 2023. Optimizing the lysis step in CTAB DNA extractions of silica-dried and herbarium leaf tissues. Appl Plant Sci 11: e11522.
-
26.
Tomasek O , Kubicek O , Tukac V , 2008. Comparison of three template preparation methods for routine detection of beak and feather disease virus and avian polyomavirus with single and nested polymerase chain reaction in clinical specimens. Avian Pathol 37: 145–149.
-
27.
Vivantis , 2023. GF-1 Blood DNA Extraction Kit. Available at: https://www.vivantechnolo-gies.com/images/Resources/roomtempmanual/gf%201%20blood%20dna%20extraction%20kit.pdf. Accessed July 12, 2023.
-
28.
Molecular Research Center , 2015. DNAzol®Direct. Available at: https://www.mrcgene.com/wp-content/uploads/2014/06/DNAzolDirectApril2015.pdf. Accessed January 14, 2023.
-
29.
Miguel RB , Coura JR , Samudio F , Suárez-Mutis MC , 2013. Evaluation of three different DNA extraction methods from blood samples collected in dried filter paper in Plasmodium sub-patent infections from the Amazon region in Brazil. Rev Inst Med Trop São Paulo 55: 205–208.
-
30.
Singhanetra-Renard A , 1993. Malaria and mobility in Thailand. Soc Sci Med 37: 1147–1154.
-
31.
World Health Organization , 2010. Malaria in the Greater Mekong Subregion: Regional and Country Profiles. Available at: https://iris.who.int/handle/10665/205432. Accessed October 28, 2023.
-
32.
Parker DM et al., 2015. Microgeography and molecular epidemiology of malaria at the Thailand-Myanmar border in the malaria pre-elimination phase. Malar J 14: 198.
-
33.
Sattabongkot J , Suansomjit C , Nguitragool W , Sirichaisinthop J , Warit S , Tiensuwan M , Buates S , 2018. Prevalence of asymptomatic Plasmodium infections with sub-microscopic parasite densities in the northwestern border of Thailand: a potential threat to malaria elimination. Malar J 17: 329.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1372 | 1066 | 66 |
| Full Text Views | 457 | 76 | 6 |
| PDF Downloads | 308 | 99 | 6 |