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RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
World Health Organization , 2023. Chagas Disease (American Trypanosomiasis). Available at: https://www.who.int/health-topics/chagas-disease. Accessed September 5, 2023.
-
2.
Zingales B , 2018. Trypanosoma cruzi genetic diversity: something new for something known about Chagas disease manifestations, serodiagnosis and drug sensitivity. Acta Trop 184: 38–52.
-
3.
Bruneto EG et al., 2021. Case-fatality from orally-transmitted acute Chagas disease: a systematic review and meta-analysis. Clin Infect Dis 72: 1084–1092.
-
4.
Hernández C et al., 2016. High-resolution molecular typing of Trypanosoma cruzi in 2 large outbreaks of acute Chagas disease in Colombia. J Infect Dis 214: 1252–1255.
-
5.
Hernández C , Cucunubá Z , Flórez C , Olivera M , Valencia C , Zambrano P , León C , Ramírez JD , 2016. Molecular diagnosis of Chagas disease in Colombia: parasitic loads and discrete typing units in patients from acute and chronic phases. PLoS Negl Trop Dis 10: e0004997.
-
6.
Marcon GEB , Ferreira JJG , de Almeida EA , Delicio AM , Pereira MB , Wanderley JDS , Martins LC , Andrade PD , de Lima RG , Costa SCB , 2022. Parasite load evaluation by qPCR and blood culture in Chagas disease and HIV co-infected patients under antiretroviral therapy. PLoS Negl Trop Dis 16: e0010317.
-
7.
Duffy T et al., 2013. Analytical performance of a multiplex real-time PCR assay using TaqMan probes for quantification of Trypanosoma cruzi satellite DNA in blood samples. PLoS Negl Trop Dis 7: e2000.
-
8.
Ramírez JD , Montilla M , Cucunubá ZM , Floréz AC , Zambrano P , Guhl F , 2013. Molecular epidemiology of human oral Chagas disease outbreaks in Colombia. PLoS Negl Trop Dis 7: e2041.
-
9.
Muñoz-Calderón A , Silva-Gomes NL , Apodaca S , Alarcón de Noya B , Díaz-Bello Z , Souza LRQ , Costa ADT , Britto C , Moreira OC , Schijman AG , 2021. Toward the establishment of a single standard curve for quantification of Trypanosoma cruzi natural populations using a synthetic satellite unit DNA sequence. J Mol Diagn 23: 521–531.
-
10.
Esper HR , de Freitas VLT , Assy JGPL , Shimoda EY , Berreta OCP , Lopes MH , França FOS , 2019. Fatal evolution of acute Chagas disease in a child from northern Brazil: factors that determine poor prognosis. Rev Inst Med Trop São Paulo 61: e27.
-
11.
Franco-Paredes C , Villamil-Gómez WE , Schultz J , Henao-Martínez AF , Parra-Henao G , Rassi A Jr. , Rodríguez-Morales AJ , Suarez JA , 2020. A deadly feast: elucidating the burden of orally acquired acute Chagas disease in Latin America: public health and travel medicine importance. Travel Med Infect Dis 36: 101565.
-
12.
Cantillo-Barraza O , Medina M , Zuluaga S , Blanco MI , Caro R , Jaimes-Dueñez J , Beltrán V , Xavier SC , Triana-Chavez O , 2021. Distribution and natural infection status of synantrophic triatomines (Hemiptera: Reduviidae), vectors of Trypanosoma cruzi, reveals new epidemiological scenarios for chagas disease in the highlands of Colombia. PLoS Negl Trop Dis 15: e0009574.
-
13.
Alarcón de Noya B , Díaz-Bello Z , Ruiz-Guevara R , Noya O , 2022. Chagas disease expands its epidemiological frontiers from rural to urban areas. Front Trop Dis 3: 799009.
-
14.
Zingales B , Bartholomeu DC , 2022. Trypanosoma cruzi genetic diversity: impact on transmission cycles and Chagas disease. Mem Inst Oswaldo Cruz 117: e210193.
-
15.
Luquetti A , 2019. Evaluación Internacional de la Situación Epidemiológica y de Control de Chagas en 34 Municipios de los Departamentos de Arauca, Boyacá, Casanare, Norte Santander, Santander y Vichada. Colombia: Organización Panamericana de la Salud & Organización Munidal de la Salud.
-
16.
Shikanai-Yasuda MA , Carvalho NB , 2012. Oral transmission of Chagas disease. Clin Infect Dis 54: 845–852.
-
17.
Jaimes-Dueñez J , Jiménez-Leaño ÁP , Esteban-Mendoza M , Moreno-Salcedo LA , Triana-Chávez O , Cantillo-Barraza O , 2020. Epidemiological and clinical characteristics of Trypanosoma cruzi infection in dogs (Canis lupus familiaris) from a Chagas disease-endemic urban area in Colombia. Prev Vet Med 182: 105093.
-
18.
Jaimes-Dueñez J , Triana-Chávez O , Cantillo-Barraza O , Hernández C , Ramírez JD , Góngora-Orjuela A , 2017. Molecular and serological detection of Trypanosoma cruzi in dogs (Canis lupus familiaris) suggests potential transmission risk in areas of recent acute Chagas disease outbreaks in Colombia. Prev Vet Med 141: 1–6.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 2879 | 2879 | 304 |
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| PDF Downloads | 127 | 127 | 9 |
An Outbreak of Acute Chagas Disease Possibly Spread through Oral Transmission Involving Animal Reservoirs in Eastern Colombia
Stivenn A. Gutiérrez
Stivenn A. Gutiérrez
Centro de Investigaciones en Microbiología y Biotecnología-UR, Facultad de Ciencias Naturales, Universidad del Rosario, Bogota, Colombia;
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Jeiczon Jaimes-Dueñez
Jeiczon Jaimes-Dueñez
Centro de Investigaciones en Microbiología y Biotecnología-UR, Facultad de Ciencias Naturales, Universidad del Rosario, Bogota, Colombia;
Grupo de Investigación en Ciencias Animales, Facultad de Medicina Veterinaria y Zootecnia, Universidad Cooperativa de Colombia, Bucaramanga, Colombia;
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Grupo de Investigación en Ciencias Animales, Facultad de Medicina Veterinaria y Zootecnia, Universidad Cooperativa de Colombia, Bucaramanga, Colombia;
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Lissa Cruz-Saavedra
Lissa Cruz-Saavedra
Centro de Investigaciones en Microbiología y Biotecnología-UR, Facultad de Ciencias Naturales, Universidad del Rosario, Bogota, Colombia;
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Carolina Hernández
Carolina Hernández
Centro de Investigaciones en Microbiología y Biotecnología-UR, Facultad de Ciencias Naturales, Universidad del Rosario, Bogota, Colombia;
Centro de Tecnología en Salud, Innovaseq SAS, Bogota, Colombia;
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Centro de Tecnología en Salud, Innovaseq SAS, Bogota, Colombia;
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Omar Cantillo-Barraza
Omar Cantillo-Barraza
Grupo BCEI, Universidad de Antioquia, Medellin, Colombia;
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Francisco Álvarez
Francisco Álvarez
Programa de Control de ETV, Secretaría de Salud de Boyacá, Tunja, Colombia;
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María Blanco
María Blanco
Secretaría Departamental de Salud de Arauca, Arauca, Colombia;
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Bernardo Leal
Bernardo Leal
Programa de Control de ETV, Secretaría de Salud de Boyacá, Tunja, Colombia;
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Lida Martínez
Lida Martínez
Grupo de Vigilancia en Salud Pública, Secretaría de Salud de Boyacá, Colombia;
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Manuel Medina
Manuel Medina
Programa de Control de ETV, Secretaría de Salud de Boyacá, Tunja, Colombia;
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Mabel Medina
Mabel Medina
Programa de Control de ETV, Secretaría de Salud de Boyacá, Tunja, Colombia;
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Silvia Valdivieso
Silvia Valdivieso
Secretaría Departamental de Salud de Arauca, Arauca, Colombia;
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Juan David Ramírez
Juan David Ramírez
Centro de Investigaciones en Microbiología y Biotecnología-UR, Facultad de Ciencias Naturales, Universidad del Rosario, Bogota, Colombia;
Molecular Microbiology Laboratory, Department of Pathology, Molecular and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, New York
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Molecular Microbiology Laboratory, Department of Pathology, Molecular and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, New York
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ABSTRACT.
Chagas disease (CD) is a parasitic infection caused by the parasite Trypanosoma cruzi. Reports of CD cases associated with oral transmission have increased, particularly in Colombia, Brazil, and Venezuela. In this investigation, parasitological, serological, and molecular tests were conducted on samples obtained from humans, mammal reservoirs, and hosts involved in the assessment of a suspected oral transmission outbreak in Cubara, Boyaca, Colombia. Seropositivity was observed in 60% (3 of 5) of index patients and 6.4% (5 of 78) of close contacts. Trypanosoma cruzi DNA was detected by quantitative polymerase chain reaction in 100% of index cases, 6.4% (5 of 78) of close contacts, 60% (6 of 10) of canines, and 100% (5 of 5) of opossums. In all index cases, the TcI lineage was identified, along with two cases of mixed infection (TcI/TcII–TcVI). Hemoculture revealed a flagellate presence in 80% of opossums, whereas all triatomine bugs tested negative. Our findings suggest a potential oral transmission route through contamination with opossum secretions.
Author Notes
Financial support: This work was funded by the
Authors’ addresses: Stivenn A. Gutiérrez and Lissa Cruz-Saavedra, Centro de Investigaciones en Microbiología y Biotecnología-UR, Facultad de Ciencias Naturales, Universidad del Rosario, Bogota, Colombia, E-mails: stivenn.gutierrez@urosario.edu.co and lissa9115@gmail.co. Jeiczon Jaimes-Dueñez, Centro de Investigaciones en Microbiología y Biotecnología-UR, Facultad de Ciencias Naturales, Universidad del Rosario, Bogota, Colombia, and Grupo de Investigación en Ciencias Animales, Facultad de Medicina Veterinaria y Zootecnia, Universidad Cooperativa de Colombia, Bucaramanga, Colombia, E-mail: jeiczon.jaimes@campusucc.edu.co. Carolina Hernández, Centro de Investigaciones en Microbiología y Biotecnología-UR, Facultad de Ciencias Naturales, Universidad del Rosario, Bogota, Colombia, and Centro de Tecnología en Salud, Innovaseq SAS, Bogota, Colombia, E-mail: dcahernandezc@gmail.com. Omar Cantillo-Barraza, Grupo BCEI, Universidad de Antioquia, Medellin, Colombia, E-mail: omarcantillo@gmail.com. Francisco Álvarez, Bernardo Leal, Manuel Medina, and Mabel Medina, Programa de Control de ETV, Secretaría de Salud de Boyacá, Tunja, Colombia, E-mails: mano.franciscoalvarezo@gmail.com, berleal9@hotmail.com, medica349@gmail.com, and mabel.medina@boyaca.gov.co. María Blanco and Silvia Valdivieso, Secretaría Departamental de Salud de Arauca, Arauca, Colombia, E-mails: miblancon@gmail.com and silviajulianavaldivieso@gmail.com. Lida Martínez, Grupo de Vigilancia en Salud Pública, Secretaría de Salud de Boyacá, Colombia, E-mail: lidamusdie@gmail.com. Juan David Ramírez, Centro de Investigaciones en Microbiología y Biotecnología-UR, Facultad de Ciencias Naturales, Universidad del Rosario, Bogota, Colombia, and Molecular Microbiology Laboratory, Department of Pathology, Molecular and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, E-mail: juand.ramirez@urosario.edu.co.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
World Health Organization , 2023. Chagas Disease (American Trypanosomiasis). Available at: https://www.who.int/health-topics/chagas-disease. Accessed September 5, 2023.
-
2.
Zingales B , 2018. Trypanosoma cruzi genetic diversity: something new for something known about Chagas disease manifestations, serodiagnosis and drug sensitivity. Acta Trop 184: 38–52.
-
3.
Bruneto EG et al., 2021. Case-fatality from orally-transmitted acute Chagas disease: a systematic review and meta-analysis. Clin Infect Dis 72: 1084–1092.
-
4.
Hernández C et al., 2016. High-resolution molecular typing of Trypanosoma cruzi in 2 large outbreaks of acute Chagas disease in Colombia. J Infect Dis 214: 1252–1255.
-
5.
Hernández C , Cucunubá Z , Flórez C , Olivera M , Valencia C , Zambrano P , León C , Ramírez JD , 2016. Molecular diagnosis of Chagas disease in Colombia: parasitic loads and discrete typing units in patients from acute and chronic phases. PLoS Negl Trop Dis 10: e0004997.
-
6.
Marcon GEB , Ferreira JJG , de Almeida EA , Delicio AM , Pereira MB , Wanderley JDS , Martins LC , Andrade PD , de Lima RG , Costa SCB , 2022. Parasite load evaluation by qPCR and blood culture in Chagas disease and HIV co-infected patients under antiretroviral therapy. PLoS Negl Trop Dis 16: e0010317.
-
7.
Duffy T et al., 2013. Analytical performance of a multiplex real-time PCR assay using TaqMan probes for quantification of Trypanosoma cruzi satellite DNA in blood samples. PLoS Negl Trop Dis 7: e2000.
-
8.
Ramírez JD , Montilla M , Cucunubá ZM , Floréz AC , Zambrano P , Guhl F , 2013. Molecular epidemiology of human oral Chagas disease outbreaks in Colombia. PLoS Negl Trop Dis 7: e2041.
-
9.
Muñoz-Calderón A , Silva-Gomes NL , Apodaca S , Alarcón de Noya B , Díaz-Bello Z , Souza LRQ , Costa ADT , Britto C , Moreira OC , Schijman AG , 2021. Toward the establishment of a single standard curve for quantification of Trypanosoma cruzi natural populations using a synthetic satellite unit DNA sequence. J Mol Diagn 23: 521–531.
-
10.
Esper HR , de Freitas VLT , Assy JGPL , Shimoda EY , Berreta OCP , Lopes MH , França FOS , 2019. Fatal evolution of acute Chagas disease in a child from northern Brazil: factors that determine poor prognosis. Rev Inst Med Trop São Paulo 61: e27.
-
11.
Franco-Paredes C , Villamil-Gómez WE , Schultz J , Henao-Martínez AF , Parra-Henao G , Rassi A Jr. , Rodríguez-Morales AJ , Suarez JA , 2020. A deadly feast: elucidating the burden of orally acquired acute Chagas disease in Latin America: public health and travel medicine importance. Travel Med Infect Dis 36: 101565.
-
12.
Cantillo-Barraza O , Medina M , Zuluaga S , Blanco MI , Caro R , Jaimes-Dueñez J , Beltrán V , Xavier SC , Triana-Chavez O , 2021. Distribution and natural infection status of synantrophic triatomines (Hemiptera: Reduviidae), vectors of Trypanosoma cruzi, reveals new epidemiological scenarios for chagas disease in the highlands of Colombia. PLoS Negl Trop Dis 15: e0009574.
-
13.
Alarcón de Noya B , Díaz-Bello Z , Ruiz-Guevara R , Noya O , 2022. Chagas disease expands its epidemiological frontiers from rural to urban areas. Front Trop Dis 3: 799009.
-
14.
Zingales B , Bartholomeu DC , 2022. Trypanosoma cruzi genetic diversity: impact on transmission cycles and Chagas disease. Mem Inst Oswaldo Cruz 117: e210193.
-
15.
Luquetti A , 2019. Evaluación Internacional de la Situación Epidemiológica y de Control de Chagas en 34 Municipios de los Departamentos de Arauca, Boyacá, Casanare, Norte Santander, Santander y Vichada. Colombia: Organización Panamericana de la Salud & Organización Munidal de la Salud.
-
16.
Shikanai-Yasuda MA , Carvalho NB , 2012. Oral transmission of Chagas disease. Clin Infect Dis 54: 845–852.
-
17.
Jaimes-Dueñez J , Jiménez-Leaño ÁP , Esteban-Mendoza M , Moreno-Salcedo LA , Triana-Chávez O , Cantillo-Barraza O , 2020. Epidemiological and clinical characteristics of Trypanosoma cruzi infection in dogs (Canis lupus familiaris) from a Chagas disease-endemic urban area in Colombia. Prev Vet Med 182: 105093.
-
18.
Jaimes-Dueñez J , Triana-Chávez O , Cantillo-Barraza O , Hernández C , Ramírez JD , Góngora-Orjuela A , 2017. Molecular and serological detection of Trypanosoma cruzi in dogs (Canis lupus familiaris) suggests potential transmission risk in areas of recent acute Chagas disease outbreaks in Colombia. Prev Vet Med 141: 1–6.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 2879 | 2879 | 304 |
| Full Text Views | 122 | 122 | 7 |
| PDF Downloads | 127 | 127 | 9 |