ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
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1.
Abebe GM , 2020. Emerging and re-Emerging viral diseases: the case of coronavirus disease-19 (COVID-19). Int J Virol AIDS 7: 067.
-
2.
Pierson TC , Diamond MS , 2020. The continued threat of emerging flaviviruses. Nat Microbiol 5: 796–812.
-
3.
Farinelli EC , Baquero OS , Stephan C , Chiaravalloti-Neto F , 2018. Low socioeconomic condition and the risk of dengue fever: a direct relationship. Acta Trop 180: 47–57.
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4.
Pan American Health Organization, Plataforma de Información en Salud de las Américas , 2020. Dengue y Dengue Grave, Casos y Muertes para Subregiones de las Américas. Available at: https://www.paho.org/data/index.php/es/temas/indicadores-dengue/dengue-regional/506-dengue-reg-ano-es.html. Accessed June 13, 2022.
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Weaver SC , Charlier C , Vasilakis N , Lecuit M , 2018. Zika, chikungunya, and other emerging vector-borne viral diseases. Annu Rev Med 69: 395–408.
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Higuera A , Ramírez JD , 2019. Molecular epidemiology of dengue, yellow fever, Zika and chikungunya arboviruses: an update. Acta Trop 190: 99–111.
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7.
Baroncelli S et al., 2021. Dried blood spots for the quantitative evaluation of IgG isotypes and correlation with serum samples in HIV-exposed uninfected (HEU) infants. J Immunol Methods 493: 113019.
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8.
Anders KL et al., 2012. An evaluation of dried blood spots and oral swabs as alternative specimens for the diagnosis of dengue and screening for past dengue virus exposure. Am J Trop Med Hyg 87: 165–170.
-
9.
Yamamoto C et al., 2020. Evaluation of the efficiency of dried blood spot-based measurement of hepatitis B and hepatitis C virus seromarkers. Sci Rep 10: 3857.
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10.
Riddell MA , Leydon JA , Catton MG , Kelly HA , 2002. Detection of measles virus-specific immunoglobulin M in dried venous blood samples by using a commercial enzyme immunoassay. J Clin Microbiol 40: 5–9.
-
11.
Toh ZQ et al., 2021. The use of dried blood spots for the serological evaluation of SARS-CoV-2 antibodies. J Public Health 44: e260–e263.
-
12.
Hegazy MM , Hegazy MK , Azab MS , Nabih N , 2020. Validation of dried blood spots in monitoring toxoplasmosis. Pathog Glob Health 114: 242–250.
-
13.
Whittaker K , Mao YQ , Lin Y , Zhang H , Zhu S , Peck H , Huang RP , 2021. Dried blood sample analysis by antibody array across the total testing process. Sci Rep 11: 20549.
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14.
McAllister G , Shepherd S , Templeton K , Aitken C , Gunson R , 2015. Long term stability of HBsAg, anti-HBc and anti-HCV in dried blood spot samples and eluates. J Clin Virol 71: 10–17.
-
15.
Amini F , Auma E , Hsia Y , Bilton S , Hall T , Ramkhelawon L , Heath PT , le Doare K , 2021. Reliability of dried blood spot (DBS) cards in antibody measurement: a systematic review. PLoS One 16: e0248218.
-
16.
McDade TW et al., 2020. High seroprevalence for SARS-CoV-2 among household members of essential workers detected using a dried blood spot assay. PLoS One 15: e0237833.
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17.
Maldonado-Rodríguez A , Rojas-Montes O , Vazquez-Rosales G , Chavez-Negrete A , Rojas-Uribe M , Posadas-Mondragon A , Aguilar-Faisal L , Cevallos AM , Xoconostle-Cazares B , Lira R , 2017. Serum dried samples to detect dengue antibodies: a field study. BioMed Res Int 2017: 7215259.
-
18.
Dirección General de Epidemiologia, Sistema Nacional de Vigilancia Epidemiológica , 2015. Histórico Boletín Epidemiológico, Semana Epidemiológica 52, 2015. Available at https://www.gob.mx/salud/acciones-y-programas/historico-boletin-epidemiologico. Accessed March 24, 2023.
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19.
Meyers E et al., 2021. Comparison of dried blood spots and venous blood for the detection of SARS-CoV-2 antibodies in a population of nursing home residents. Microbiol Spectr 9: e0017821.
-
20.
Chanama S , Anantapreecha S , A-nuegoonpipat A , Sa-gnasang A , Kurane I , Sawanpanyalert P , 2004. Analysis of specific IgM responses in secondary dengue virus infections: levels and positive rates in comparison with primary infections. J Clin Virol 31: 185–189.
-
21.
Tran TNT et al., 2006. Enzyme-linked immunoassay for dengue virus IgM and IgG antibodies in serum and filter paper blood. BMC Infect Dis 6: 13.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 2445 | 850 | 98 |
| Full Text Views | 214 | 60 | 1 |
| PDF Downloads | 75 | 35 | 1 |
Dried Serum Samples for Antibody Detection in Arthropod-Borne Virus Infections Are an Effective Alternative to Serum Samples
Daniela Würsch
Daniela Würsch
Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Unidad Médica de Alta Especialidad Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico;
Programa de Doctorado en Ciencias Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico;
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Programa de Doctorado en Ciencias Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico;
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Othon Rojas-Montes
Othon Rojas-Montes
Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Unidad Médica de Alta Especialidad Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico;
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Angélica Maldonado-Rodríguez
Angélica Maldonado-Rodríguez
Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Unidad Médica de Alta Especialidad Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico;
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Edgar Sevilla-Reyes
Edgar Sevilla-Reyes
Laboratorio de Transcriptómica e Inmunología Molecular, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Ciudad de Mexico, Mexico;
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Ana María Cevallos
Ana María Cevallos
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico City, Mexico;
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Gilma Sánchez-Burgos
Gilma Sánchez-Burgos
Unidad de Investigación Médica Yucatán, Instituto Mexicano del Seguro Social, Mérida, Mexico;
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Adolfo Chávez-Negrete
Adolfo Chávez-Negrete
Educación e Investigación en Salud, Unidad Médica de Alta Especialidad Hospital de Especialidades, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico
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Rosalía Lira
Rosalía Lira
Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Unidad Médica de Alta Especialidad Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico;
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ABSTRACT.
The disease burden of arthropod-borne infections is particularly high in low- and middle-income countries, where the availability of resources for surveillance and testing is limited. The lack of local infrastructure demands that biological samples be sent to central laboratories by refrigerated transport, which increases costs and the risk of sample degradation. Dried blood spot samples are an alternative for ensuring sample integrity during transportation and storage. They can be used for the detection of nucleic acids and proteins, such as antigens or antibodies. Here, we compared anti-chikungunya IgM, anti-dengue IgM, anti-dengue IgG, and anti-Zika IgG detection between paired serum and dried serum samples (DSSs); the agreement between results was found to be 90.6%, 94.1%, 85.9%, and 95.5%, respectively, indicating a strong correlation. Our results suggest that DSSs provide a reliable alternative for detection of specific antibodies in arthropod-borne infections.
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Author Notes
Financial support: This work was supported by a grant from
Authors’ addresses: Daniela Würsch, Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Unidad Médica de Alta Especialidad Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico, and Programa de Doctorado en Ciencias Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico, E-mail: dwurschmolina@gmail.com. Othon Rojas-Montes, Angélica Maldonado-Rodríguez, and Rosalía Lira, Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Unidad Médica de Alta Especialidad Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico, E-mails: othonrojas@yahoo.com, mangimr@yahoo.com.mx, and drarosalialira@gmail.com. Edgar Sevilla-Reyes, Laboratorio de Transcriptómica e Inmunología Molecular, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Ciudad de Mexico, Mexico, E-mail: edgar.sevilla@gmail.com. Ana María Cevallos, Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico City, Mexico, E-mail: amcevallos@biomedicas.unam.mx. Gilma Sánchez-Burgos, Unidad de Investigación Médica Yucatán, Instituto Mexicano del Seguro Social, Mérida, Mexico, E-mail: gilmagburgos@gmail.com. Adolfo Chávez-Negrete, Educación e Investigación en Salud, Unidad Médica de Alta Especialidad Hospital de Especialidades, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico, E-mail: achavezn@gmail.com.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Abebe GM , 2020. Emerging and re-Emerging viral diseases: the case of coronavirus disease-19 (COVID-19). Int J Virol AIDS 7: 067.
-
2.
Pierson TC , Diamond MS , 2020. The continued threat of emerging flaviviruses. Nat Microbiol 5: 796–812.
-
3.
Farinelli EC , Baquero OS , Stephan C , Chiaravalloti-Neto F , 2018. Low socioeconomic condition and the risk of dengue fever: a direct relationship. Acta Trop 180: 47–57.
-
4.
Pan American Health Organization, Plataforma de Información en Salud de las Américas , 2020. Dengue y Dengue Grave, Casos y Muertes para Subregiones de las Américas. Available at: https://www.paho.org/data/index.php/es/temas/indicadores-dengue/dengue-regional/506-dengue-reg-ano-es.html. Accessed June 13, 2022.
-
5.
Weaver SC , Charlier C , Vasilakis N , Lecuit M , 2018. Zika, chikungunya, and other emerging vector-borne viral diseases. Annu Rev Med 69: 395–408.
-
6.
Higuera A , Ramírez JD , 2019. Molecular epidemiology of dengue, yellow fever, Zika and chikungunya arboviruses: an update. Acta Trop 190: 99–111.
-
7.
Baroncelli S et al., 2021. Dried blood spots for the quantitative evaluation of IgG isotypes and correlation with serum samples in HIV-exposed uninfected (HEU) infants. J Immunol Methods 493: 113019.
-
8.
Anders KL et al., 2012. An evaluation of dried blood spots and oral swabs as alternative specimens for the diagnosis of dengue and screening for past dengue virus exposure. Am J Trop Med Hyg 87: 165–170.
-
9.
Yamamoto C et al., 2020. Evaluation of the efficiency of dried blood spot-based measurement of hepatitis B and hepatitis C virus seromarkers. Sci Rep 10: 3857.
-
10.
Riddell MA , Leydon JA , Catton MG , Kelly HA , 2002. Detection of measles virus-specific immunoglobulin M in dried venous blood samples by using a commercial enzyme immunoassay. J Clin Microbiol 40: 5–9.
-
11.
Toh ZQ et al., 2021. The use of dried blood spots for the serological evaluation of SARS-CoV-2 antibodies. J Public Health 44: e260–e263.
-
12.
Hegazy MM , Hegazy MK , Azab MS , Nabih N , 2020. Validation of dried blood spots in monitoring toxoplasmosis. Pathog Glob Health 114: 242–250.
-
13.
Whittaker K , Mao YQ , Lin Y , Zhang H , Zhu S , Peck H , Huang RP , 2021. Dried blood sample analysis by antibody array across the total testing process. Sci Rep 11: 20549.
-
14.
McAllister G , Shepherd S , Templeton K , Aitken C , Gunson R , 2015. Long term stability of HBsAg, anti-HBc and anti-HCV in dried blood spot samples and eluates. J Clin Virol 71: 10–17.
-
15.
Amini F , Auma E , Hsia Y , Bilton S , Hall T , Ramkhelawon L , Heath PT , le Doare K , 2021. Reliability of dried blood spot (DBS) cards in antibody measurement: a systematic review. PLoS One 16: e0248218.
-
16.
McDade TW et al., 2020. High seroprevalence for SARS-CoV-2 among household members of essential workers detected using a dried blood spot assay. PLoS One 15: e0237833.
-
17.
Maldonado-Rodríguez A , Rojas-Montes O , Vazquez-Rosales G , Chavez-Negrete A , Rojas-Uribe M , Posadas-Mondragon A , Aguilar-Faisal L , Cevallos AM , Xoconostle-Cazares B , Lira R , 2017. Serum dried samples to detect dengue antibodies: a field study. BioMed Res Int 2017: 7215259.
-
18.
Dirección General de Epidemiologia, Sistema Nacional de Vigilancia Epidemiológica , 2015. Histórico Boletín Epidemiológico, Semana Epidemiológica 52, 2015. Available at https://www.gob.mx/salud/acciones-y-programas/historico-boletin-epidemiologico. Accessed March 24, 2023.
-
19.
Meyers E et al., 2021. Comparison of dried blood spots and venous blood for the detection of SARS-CoV-2 antibodies in a population of nursing home residents. Microbiol Spectr 9: e0017821.
-
20.
Chanama S , Anantapreecha S , A-nuegoonpipat A , Sa-gnasang A , Kurane I , Sawanpanyalert P , 2004. Analysis of specific IgM responses in secondary dengue virus infections: levels and positive rates in comparison with primary infections. J Clin Virol 31: 185–189.
-
21.
Tran TNT et al., 2006. Enzyme-linked immunoassay for dengue virus IgM and IgG antibodies in serum and filter paper blood. BMC Infect Dis 6: 13.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 2445 | 850 | 98 |
| Full Text Views | 214 | 60 | 1 |
| PDF Downloads | 75 | 35 | 1 |