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Cytogenetic Key to Identification of Triatominae Species Reported in an Outbreak Region of Oral Transmission of Chagas Disease in the Brazilian Northeast

Emercio Felisberto Nhapulo Laboratório de Entomologia em Saúde Pública, Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, Brazil;

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Denis Vinícius de Mello Instituto de Biociências, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Botucatu, Brazil;

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Laura Poloto Cesaretto Instituto de Biociências, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Botucatu, Brazil;

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Julia Junqueira Alevi Instituto de Biociências, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Botucatu, Brazil;

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Daniel Cesaretto Cristal Instituto de Biociências, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Botucatu, Brazil;

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Giulia Montanari Instituto de Biociências, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Botucatu, Brazil;

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Luísa Martins Sensato Azevedo Laboratório de Biologia Celular, Instituto de Biociências, Letras e Ciências Exatas, UNESP, São José do Rio Preto, Brazil;

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Isabella da Silva Masarin Instituto de Biociências, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Botucatu, Brazil;

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Cleber Galvão Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil

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Kaio Cesar Chaboli Alevi Laboratório de Entomologia em Saúde Pública, Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, Brazil;
Instituto de Biociências, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Botucatu, Brazil;
Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil

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DOI:
https://doi.org/10.4269/ajtmh.22-0697
Volume/Issue:
Volume 108: Issue 6
Page(s):
1161–1163
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ABSTRACT.

Oral transmission from the consumption of processed food with triatomines and/or their feces infected with Trypanosoma cruzi prevails among recent cases of Chagas disease in Brazil. In Paraíba, a state of the Brazilian northeast, there was an outbreak caused by the consumption of sugarcane juice that resulted in 26 cases of infection and one death. Until now, 10 species of triatomines have been reported in this Brazilian state. Thus, we developed a dichotomous key to assist in the correct identification of Paraíba triatomines based on cytogenetic data. The dichotomous key allowed the differentiation of all the species in this state. Although the purpose of CytoKeys is not to replace dichotomous keys based on morphological data, the use of these complementary keys can help to solve taxonomic problems, preventing identification errors, especially between similar species such as Triatoma brasiliensis and Triatoma petrocchiae, both present in the Brazilian northeast.

Author Notes

Address correspondence to Cleber Galvão, Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Instituto Oswaldo Cruz, Av. Brasil 4365, Pavilhão Rocha Lima, Sala 505, 21040-360 Rio de Janeiro, RJ, Brazil. E-mail: clebergalvao@gmail.com

Authors’ addresses: Emercio Felisberto Nhapulo, Laboratório de Entomologia em Saúde Pública, Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, Brazil, E-mail: emercionhapulo@usp.br. Denis Vinícius de Mello, Laura Poloto Cesaretto, Julia Junqueira Alevi, Daniel Cesaretto Cristal, Giulia Montanari, and Isabella da Silva Masarin, Instituto de Biociências, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Botucatu, Brazil, E-mails: denis.mello@alumni.usp.br; lauracessretto@icloud.com; juliaalevi6@gmail.com; daniel.cristal@hotmail.com; giuliamontanari@hotmail.com; isabella.masarin@unesp.br. Luísa Martins Sensato Azevedo, Laboratório de Biologia Celular, Instituto de Biociências, Letras e Ciências Exatas, UNESP, São José do Rio Preto, Brazil, E-mail: luisa.sensato@unesp.br. Cleber Galvão, Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil, E-mail: clebergalvao@gmail.com. Kaio Cesar Chaboli Alevi, Laboratório de Entomologia em Saúde Pública, Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, Brazil, Instituto de Biociências, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Botucatu, Brazil, and Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil, E-mail: kaiochaboli@hotmail.com.

Copyright:
© The American Society of Tropical Medicine and Hygiene 2023
Received:
05 Nov 2022
|
Accepted:
25 Jan 2023
|
Published Online:
09 May 2023
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Online ISSN:
1476-1645
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