Author:
ProCite
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Reference Manager
BibTeX
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-
1.
Ruiz-Calero Cendrero RM , Cancho Castellano B , MartÃn Hidalgo-Barquero MV , Cid Parra MC , Galán González J , Fernández SolÃs MA , Sánchez GarcÃa O , 2020. When can it be useful to look for occult HBV in haemodialysis patients? Nefrologia 40: 115–119.
-
2.
Hedayati-Moghaddam MR , Soltanian H , Behzadifar M , 2020. Occult hepatitis B virus infection prevalence among different populations of Iran: a systematic review and meta-analysis. Hepat Mon 20: e101722.
-
3.
Beykaso G , Mulu A , Giday M , Berhe N , Selamu M , Hailu D , Teklehaymanot T , 2022. Occult hepatitis B virus infection and its risks of cryptic transmission in southern Ethiopia. Infect Drug Resist 15: 619–630.
-
4.
Kwak M-S , Kim YJ , 2014. Occult hepatitis B virus infection. World J Hepatol 6: 860–869.
-
5.
Aghakhani A , Banifazl M , Velayati AA , Eslamifar A , Ramezani A , 2012. Occult hepatitis B virus infection in hemodialysis patients: a concept for consideration. Ther Apher Dial 16: 328–333.
-
6.
Fontenele AM , Filho NS , Ferreira AS , 2013. Occult hepatitis B in patients on hemodialysis: a review. Ann Hepatol 12: 527–531.
-
7.
Shokri S , Mahmoudvand S , Taherkhani R , Farshadpour F , Jalalian FA , 2019. Complexity on modulation of NF-kappaB pathways by hepatitis B and C: a double-edged sword in hepatocarcinogenesis. J Cell Physiol 234: 14734–14742.
-
8.
Mohammadi Z et al., 2016. Epidemiological profile of hepatitis B virus infection in Iran in the past 25 years; a systematic review and meta-analysis of general population studies. Middle East J Dig Dis 8: 5.
-
9.
Mahmud S , Akbarzadeh V , Abu-Raddad LJ , 2018. The epidemiology of hepatitis C virus in Iran: systematic review and meta-analyses. Sci Rep 8: 150.
-
10.
Farshadpour F , Taherkhani R , Tajbakhsh S , Gholizadeh Tangestani M , Hajiani G , Sharifi N , Taherkhani S , Nejadbolkheyr A , 2016. Prevalence and trends of transfusion-transmissible viral infections among blood donors in south of Iran: an eleven-year retrospective study. PLoS One 11: e0157615.
-
11.
Taherkhani R , Farshadpour F , 2022. Prevalence, genotype distribution and mutations of hepatitis B virus and the associated risk factors among pregnant women residing in the northern shores of Persian Gulf, Iran. PLoS One 17: e0265063.
-
12.
Farshadpour F , Taherkhani R , Saberi F , 2022. Molecular evaluation of hepatitis B virus infection and predominant mutations of pre-core, basal core promoter and S regions in an Iranian population with type 2 diabetes mellitus: a case-control study. BMC Infect Dis 22: 553.
-
13.
Farshadpour F , Taherkhani R , Ravanbod MR , Eghbali SS , 2018. Prevalence and genotype distribution of hepatitis C virus infection among patients with type 2 diabetes mellitus. Med Princ Pract 27: 308–316.
-
14.
Farshadpour F , Taherkhani R , Bakhtiari F , 2021. Prevalence and predominant genotype of hepatitis C virus infection and associated risk factors among pregnant women in Iran. BioMed Res Int 2021: 9294276.
-
15.
Farshadpour F , Taherkhani R , 2022. Prevalence and molecular evaluation of hepatitis C virus infection among multi-transfused thalassemia patients in south of Iran. Oman Med J 37: e427.
-
16.
Aghakhani A , Banifazl M , Kalantar E , Eslamifar A , Ahmadi F , Razeghi E , Atabak S , Amini M , Khadem-Sadegh A , Ramezani A , 2010. Occult hepatitis B virus infection in hemodialysis patients with isolated hepatitis B core antibody: a multicenter study. Ther Apher Dial 14: 349–353.
-
17.
Ayatollahi J , Jahanabadi S , Sharifyazdi M , Hemayati R , Vakili M , Shahcheraghi SH , 2016. The prevalence of occult hepatitis B virus in the hemodialysis patients in Yazd, Iran. Acta Med Iran 54: 784–787.
-
18.
Kalantari H , Ferdowsi F , Yaran M , 2016. Prevalence of occult hepatitis B virus infection in hemodialysis patients in Isfahan, Iran. Adv Biomed Res 5: 151.
-
19.
Joukar F , Mansour-Ghanaei F , Besharati S , Khosh-Sorur M , 2012. Occult hepatitis B infection in a hemodialysis population in Guilan province, northern Iran. Hemodial Int 16: 294–297.
-
20.
Ranjbar M , Dadkhah M , Bokharaei Salim F , Daneshbodi M , Savaj S , Keyvani H , 2017. The prevalence of occult HBV infection among hemodialysis patients of Tehran, Iran. Mod Care J 14: e65546.
-
21.
Samiei RN , Shokri S , Mahmoudvand S , Makvandi M , Shahbazian H , Shayanpur S , Nowrozi S , Nejad KH , 2018. Occult hepatitis B virus infection among hemodialysis patients. J Biol Res (Thessalon) 91: 7516.
-
22.
Samadi E , Mirshahabi H , Motamed N , Sadeghi H , 2020. Prevalence of occult hepatitis B virus infection in hemodialysis patients using nested PCR. Rep Biochem Mol Biol 9: 82–88.
-
23.
Musa MD , Ateya HK , 2020. Prevalence of overt and occult hepatitis B virus infections among 135 haemodialysis patients attending a haemodialysis centre at Al-Nasiriyah city, Iraq. Iran J Microbiol 12: 475–482.
-
24.
Nagakawa O , Miyatomi Y , Shigeta Y , Inayama E , Murakami K , Sakai T , Kouno T , Masai M , Shirai K , Yoshida T , 2013. Occult hepatitis B virus infection in Japanese chronic hemodialysis patients. Ther Apher Dial 17: 289–292.
-
25.
Mina P , Georgiadou SP , Rizos C , Dalekos GN , Rigopoulou EI , 2010. Prevalence of occult hepatitis B virus infection in haemodialysis patients from central Greece. World J Gastroenterol 16: 225–231.
-
26.
Kim SM , Kim HW , Lee JE , Lee EK , Shin HD , Song IH , 2014. Occult hepatitis B virus infection of hemodialysis patients: a cross-sectional study in a hepatitis B virus-endemic region. Hemodial Int 19: 66–71.
-
27.
Sowole L , Labbett W , Patel M , O’Riordan A , Cross J , Davenport A , Haque T , 2015. The prevalence of occult hepatitis B virus (HBV) infection in a large multi-ethnic haemodialysis cohort. BMC Nephrol 16: 12.
-
28.
Fontenele AM , Gainer JB , da Silva E Silva DV , Cruz Santos MD , Salgado JV , Salgado Filho N , Ferreira AS , 2015. Occult hepatitis B among patients with chronic renal failure on hemodialysis from a capital city in northeast Brazil. Hemodial Int 19: 353–359.
-
29.
Helaly GF , El Ghazzawi EF , Shawky SM , Farag FM , 2015. Occult hepatitis B virus infection among chronic hemodialysis patients in Alexandria, Egypt. J Infect Public Health 8: 562–569.
-
30.
Samadi E , Mirshahabi H , Montame N , Sadeghi H , 2019. Prevalence of occult hepatitis B virus (HBV) infection among hemodialysis patients in Northern State, Sudan. Virol Immunol J 9: 82–88.
-
31.
Taherkhani R , Farshadpour F , 2021. Molecular identification and clinical characteristics of mumps virus and human herpesviruses associated with aseptic meningitis in South of Iran. Virusdisease 32: 666–673.
-
32.
Hashemi SJ , Hajiani E , Masjedizadeh A , Makvandi M , Shahbazian H , Shayesteh A , Karimi M , 2015. A case–control study on occult hepatitis B infection in chronic hemodialysis patients from south-west of Iran. Govaresh 20: 135–140.
-
33.
Farshadpour F , Taherkhani R , 2021. Molecular epidemiology of enteroviruses and predominance of echovirus 30 in an Iranian population with aseptic meningitis. J Neurovirol 27: 444–451.
-
34.
Haghazali S , AslaniMehr M , Charkhchian M , Alizadeh-Kavashkoohi S , Khabaz F , Rajabkhani Z , Mehdinejad P , Mohammadi Z , 2011. Occult HBV infection in hemodialysis patients in Qazvin. RJMS 18: 8–15.
-
35.
Kanbay M , Gur G , Akcay A , Selcuk H , Yilmaz U , Arslan H , Boyacioglu S , Ozdemir FN , 2006. Is hepatitis C virus positivity a contributing factor to occult hepatitis B virus infection in hemodialysis patients? Dig Dis Sci 51: 1962–1966.
-
36.
Di Stefano M , Volpe A , Stallone G , Tartaglia L , Prato R , Martinelli D , Pastore G , Gesualdo L , Fiore JR , 2009. Occult HBV infection in hemodialysis setting is marked by presence of isolated antibodies to HBcAg and HCV. J Nephrol 22: 381–386.
-
37.
Motta JS , Mello FC , Lago BV , Perez RM , Gomes SA , Figueiredo FF , 2010. Occult hepatitis B virus infection and lamivudine-resistant mutations in isolates from renal patients undergoing hemodialysis. J Gastroenterol Hepatol 25: 101–106.
-
38.
Ismail H , Soliman M , Ismail N , 2010. Occult hepatitis B virus infection in Egyptian hemodialysis patients with or without hepatitis C virus infection. Pathol Lab Med Int 2: 113–120.
-
39.
Haghshenas MR , Arabi M , Mousavi T , 2014. Hepatitis B genotypes in Iran. Mater Sociomed 26: 129–133.
-
40.
Lin CL , Kao JH , 2015. Hepatitis B virus genotypes and variants. Cold Spring Harb Perspect Med 5: a021436.
-
41.
Sunbul M , 2014. Hepatitis B virus genotypes: global distribution and clinical importance. World J Gastroenterol 2014: 5427–5434.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 2872 | 1290 | 79 |
| Full Text Views | 403 | 40 | 0 |
| PDF Downloads | 186 | 42 | 0 |
High Prevalence of Occult Hepatitis B Virus Infection among Iranian Hemodialysis Patients
Fatemeh Farshadpour
Fatemeh Farshadpour
Department of Virology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
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Reza Taherkhani
Reza Taherkhani
Department of Virology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
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Athar Shahabi
Athar Shahabi
Department of Virology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
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ABSTRACT.
Considering the potential risks associated with occult hepatitis B virus (HBV) infection, this study was designed to investigate the magnitude and genotypic pattern of occult HBV infection among hemodialysis patients. All patients on regular hemodialysis attending the dialysis centers located in southern Iran and 277 nonhemodialysis controls were invited to participate in this study. Serum samples were tested for detection of hepatitis B core antibody (HBcAb) and hepatitis B surface antigen (HBsAg) by competitive enzyme immunoassay and sandwich ELISA, respectively. The molecular evaluation of HBV infection was conducted by two nested polymerase chain reaction (PCR) assays, targeting S, X, and precore regions of HBV genome, and sequencing by Sanger dideoxy sequencing technology. Moreover, HBV viremic samples were tested for hepatitis C virus (HCV) coinfection by HCV Ab ELISA and seminested reverse transcriptase PCR. Of 279 hemodialysis patients, five (1.8%) were positive for HBsAg, 66 (23.7%) were positive for HBcAb, and 32 (11.5%) had HBV viremia with HBV genotype D, sub-genotype D3 and subtype ayw2. Moreover, 90.6% of the hemodialysis patients with HBV viremia had occult HBV infection. Hemodialysis patients (11.5%) had significantly higher prevalence of HBV viremia than nonhemodialysis controls (1.08%; P = 0.0001). The prevalence of HBV viremia among hemodialysis patients was not statistically associated with duration of hemodialysis, age and gender distribution. In contrast, HBV viremia was significantly associated with place of residency and ethnicity, with residents of Dashtestan and Arab having had significantly higher prevalence of HBV viremia compared with the residents of other cities and Fars patients. Notably, 27.6% and 6.9% of hemodialysis patients infected with occult HBV infection were also positive for anti-HCV antibodies and HCV viremia, respectively. High prevalence of occult HBV infection was detected in hemodialysis patients, whereas 62% of patients with occult HBV infection were negative for HBcAb. Therefore, screening of all hemodialysis patients by sensitive molecular tests, regardless of the pattern of HBV serological markers, is recommended to increase the diagnosis rate of HBV infection.
Author Notes
Disclosure: This study was approved by the Ethical Committee of Bushehr University of Medical Sciences with reference number IR.BPUMS.REC.1395.181. All participants signed informed consent forms to use their serum samples for HBV detection and analysis. The participants gave written informed consent to publish their data in a journal article.
Authors’ addresses: Fatemeh Farshadpour, Reza Taherkhani, and Athar Shahabi, Department of Virology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran, E-mails: f.farshadpour@bpums.ac.ir, r.taherkhani@bpums.ac.ir, and ashahabi1996@gmail.com.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Ruiz-Calero Cendrero RM , Cancho Castellano B , MartÃn Hidalgo-Barquero MV , Cid Parra MC , Galán González J , Fernández SolÃs MA , Sánchez GarcÃa O , 2020. When can it be useful to look for occult HBV in haemodialysis patients? Nefrologia 40: 115–119.
-
2.
Hedayati-Moghaddam MR , Soltanian H , Behzadifar M , 2020. Occult hepatitis B virus infection prevalence among different populations of Iran: a systematic review and meta-analysis. Hepat Mon 20: e101722.
-
3.
Beykaso G , Mulu A , Giday M , Berhe N , Selamu M , Hailu D , Teklehaymanot T , 2022. Occult hepatitis B virus infection and its risks of cryptic transmission in southern Ethiopia. Infect Drug Resist 15: 619–630.
-
4.
Kwak M-S , Kim YJ , 2014. Occult hepatitis B virus infection. World J Hepatol 6: 860–869.
-
5.
Aghakhani A , Banifazl M , Velayati AA , Eslamifar A , Ramezani A , 2012. Occult hepatitis B virus infection in hemodialysis patients: a concept for consideration. Ther Apher Dial 16: 328–333.
-
6.
Fontenele AM , Filho NS , Ferreira AS , 2013. Occult hepatitis B in patients on hemodialysis: a review. Ann Hepatol 12: 527–531.
-
7.
Shokri S , Mahmoudvand S , Taherkhani R , Farshadpour F , Jalalian FA , 2019. Complexity on modulation of NF-kappaB pathways by hepatitis B and C: a double-edged sword in hepatocarcinogenesis. J Cell Physiol 234: 14734–14742.
-
8.
Mohammadi Z et al., 2016. Epidemiological profile of hepatitis B virus infection in Iran in the past 25 years; a systematic review and meta-analysis of general population studies. Middle East J Dig Dis 8: 5.
-
9.
Mahmud S , Akbarzadeh V , Abu-Raddad LJ , 2018. The epidemiology of hepatitis C virus in Iran: systematic review and meta-analyses. Sci Rep 8: 150.
-
10.
Farshadpour F , Taherkhani R , Tajbakhsh S , Gholizadeh Tangestani M , Hajiani G , Sharifi N , Taherkhani S , Nejadbolkheyr A , 2016. Prevalence and trends of transfusion-transmissible viral infections among blood donors in south of Iran: an eleven-year retrospective study. PLoS One 11: e0157615.
-
11.
Taherkhani R , Farshadpour F , 2022. Prevalence, genotype distribution and mutations of hepatitis B virus and the associated risk factors among pregnant women residing in the northern shores of Persian Gulf, Iran. PLoS One 17: e0265063.
-
12.
Farshadpour F , Taherkhani R , Saberi F , 2022. Molecular evaluation of hepatitis B virus infection and predominant mutations of pre-core, basal core promoter and S regions in an Iranian population with type 2 diabetes mellitus: a case-control study. BMC Infect Dis 22: 553.
-
13.
Farshadpour F , Taherkhani R , Ravanbod MR , Eghbali SS , 2018. Prevalence and genotype distribution of hepatitis C virus infection among patients with type 2 diabetes mellitus. Med Princ Pract 27: 308–316.
-
14.
Farshadpour F , Taherkhani R , Bakhtiari F , 2021. Prevalence and predominant genotype of hepatitis C virus infection and associated risk factors among pregnant women in Iran. BioMed Res Int 2021: 9294276.
-
15.
Farshadpour F , Taherkhani R , 2022. Prevalence and molecular evaluation of hepatitis C virus infection among multi-transfused thalassemia patients in south of Iran. Oman Med J 37: e427.
-
16.
Aghakhani A , Banifazl M , Kalantar E , Eslamifar A , Ahmadi F , Razeghi E , Atabak S , Amini M , Khadem-Sadegh A , Ramezani A , 2010. Occult hepatitis B virus infection in hemodialysis patients with isolated hepatitis B core antibody: a multicenter study. Ther Apher Dial 14: 349–353.
-
17.
Ayatollahi J , Jahanabadi S , Sharifyazdi M , Hemayati R , Vakili M , Shahcheraghi SH , 2016. The prevalence of occult hepatitis B virus in the hemodialysis patients in Yazd, Iran. Acta Med Iran 54: 784–787.
-
18.
Kalantari H , Ferdowsi F , Yaran M , 2016. Prevalence of occult hepatitis B virus infection in hemodialysis patients in Isfahan, Iran. Adv Biomed Res 5: 151.
-
19.
Joukar F , Mansour-Ghanaei F , Besharati S , Khosh-Sorur M , 2012. Occult hepatitis B infection in a hemodialysis population in Guilan province, northern Iran. Hemodial Int 16: 294–297.
-
20.
Ranjbar M , Dadkhah M , Bokharaei Salim F , Daneshbodi M , Savaj S , Keyvani H , 2017. The prevalence of occult HBV infection among hemodialysis patients of Tehran, Iran. Mod Care J 14: e65546.
-
21.
Samiei RN , Shokri S , Mahmoudvand S , Makvandi M , Shahbazian H , Shayanpur S , Nowrozi S , Nejad KH , 2018. Occult hepatitis B virus infection among hemodialysis patients. J Biol Res (Thessalon) 91: 7516.
-
22.
Samadi E , Mirshahabi H , Motamed N , Sadeghi H , 2020. Prevalence of occult hepatitis B virus infection in hemodialysis patients using nested PCR. Rep Biochem Mol Biol 9: 82–88.
-
23.
Musa MD , Ateya HK , 2020. Prevalence of overt and occult hepatitis B virus infections among 135 haemodialysis patients attending a haemodialysis centre at Al-Nasiriyah city, Iraq. Iran J Microbiol 12: 475–482.
-
24.
Nagakawa O , Miyatomi Y , Shigeta Y , Inayama E , Murakami K , Sakai T , Kouno T , Masai M , Shirai K , Yoshida T , 2013. Occult hepatitis B virus infection in Japanese chronic hemodialysis patients. Ther Apher Dial 17: 289–292.
-
25.
Mina P , Georgiadou SP , Rizos C , Dalekos GN , Rigopoulou EI , 2010. Prevalence of occult hepatitis B virus infection in haemodialysis patients from central Greece. World J Gastroenterol 16: 225–231.
-
26.
Kim SM , Kim HW , Lee JE , Lee EK , Shin HD , Song IH , 2014. Occult hepatitis B virus infection of hemodialysis patients: a cross-sectional study in a hepatitis B virus-endemic region. Hemodial Int 19: 66–71.
-
27.
Sowole L , Labbett W , Patel M , O’Riordan A , Cross J , Davenport A , Haque T , 2015. The prevalence of occult hepatitis B virus (HBV) infection in a large multi-ethnic haemodialysis cohort. BMC Nephrol 16: 12.
-
28.
Fontenele AM , Gainer JB , da Silva E Silva DV , Cruz Santos MD , Salgado JV , Salgado Filho N , Ferreira AS , 2015. Occult hepatitis B among patients with chronic renal failure on hemodialysis from a capital city in northeast Brazil. Hemodial Int 19: 353–359.
-
29.
Helaly GF , El Ghazzawi EF , Shawky SM , Farag FM , 2015. Occult hepatitis B virus infection among chronic hemodialysis patients in Alexandria, Egypt. J Infect Public Health 8: 562–569.
-
30.
Samadi E , Mirshahabi H , Montame N , Sadeghi H , 2019. Prevalence of occult hepatitis B virus (HBV) infection among hemodialysis patients in Northern State, Sudan. Virol Immunol J 9: 82–88.
-
31.
Taherkhani R , Farshadpour F , 2021. Molecular identification and clinical characteristics of mumps virus and human herpesviruses associated with aseptic meningitis in South of Iran. Virusdisease 32: 666–673.
-
32.
Hashemi SJ , Hajiani E , Masjedizadeh A , Makvandi M , Shahbazian H , Shayesteh A , Karimi M , 2015. A case–control study on occult hepatitis B infection in chronic hemodialysis patients from south-west of Iran. Govaresh 20: 135–140.
-
33.
Farshadpour F , Taherkhani R , 2021. Molecular epidemiology of enteroviruses and predominance of echovirus 30 in an Iranian population with aseptic meningitis. J Neurovirol 27: 444–451.
-
34.
Haghazali S , AslaniMehr M , Charkhchian M , Alizadeh-Kavashkoohi S , Khabaz F , Rajabkhani Z , Mehdinejad P , Mohammadi Z , 2011. Occult HBV infection in hemodialysis patients in Qazvin. RJMS 18: 8–15.
-
35.
Kanbay M , Gur G , Akcay A , Selcuk H , Yilmaz U , Arslan H , Boyacioglu S , Ozdemir FN , 2006. Is hepatitis C virus positivity a contributing factor to occult hepatitis B virus infection in hemodialysis patients? Dig Dis Sci 51: 1962–1966.
-
36.
Di Stefano M , Volpe A , Stallone G , Tartaglia L , Prato R , Martinelli D , Pastore G , Gesualdo L , Fiore JR , 2009. Occult HBV infection in hemodialysis setting is marked by presence of isolated antibodies to HBcAg and HCV. J Nephrol 22: 381–386.
-
37.
Motta JS , Mello FC , Lago BV , Perez RM , Gomes SA , Figueiredo FF , 2010. Occult hepatitis B virus infection and lamivudine-resistant mutations in isolates from renal patients undergoing hemodialysis. J Gastroenterol Hepatol 25: 101–106.
-
38.
Ismail H , Soliman M , Ismail N , 2010. Occult hepatitis B virus infection in Egyptian hemodialysis patients with or without hepatitis C virus infection. Pathol Lab Med Int 2: 113–120.
-
39.
Haghshenas MR , Arabi M , Mousavi T , 2014. Hepatitis B genotypes in Iran. Mater Sociomed 26: 129–133.
-
40.
Lin CL , Kao JH , 2015. Hepatitis B virus genotypes and variants. Cold Spring Harb Perspect Med 5: a021436.
-
41.
Sunbul M , 2014. Hepatitis B virus genotypes: global distribution and clinical importance. World J Gastroenterol 2014: 5427–5434.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 2872 | 1290 | 79 |
| Full Text Views | 403 | 40 | 0 |
| PDF Downloads | 186 | 42 | 0 |