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Reference Manager
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-
1.
Cantey PT , Coyle CM , Sorvillo FJ , Wilkins PP , Starr MC , Nash TE , 2014. Neglected parasitic infections in the United States: cysticercosis. Am J Trop Med Hyg 90: 805–809.
-
2.
Garcia HH , Gonzalez AE , Gilman RH , 2020. Taenia solium cysticercosis and its impact in neurological disease. Clin Microbiol Rev 33: e00085-19.
-
3.
White AC Jr et al., 2018. Diagnosis and treatment of neurocysticercosis: 2017 clinical practice guidelines by the Infectious Diseases Society of America (IDSA) and the American Society of Tropical Medicine and Hygiene (ASTMH). Nephrol Dial Transplant 66: 1159–1163.
-
4.
Bustos J , Gonzales I , Saavedra H , Handali S , Garcia HH , 2021. Neurocysticercosis: a frequent cause of seizures, epilepsy, and other neurological morbidity in most of the world. J Neurol Sci 427: 117527.
-
5.
Rodriguez S , Wilkins P , Dorny P , 2012. Immunological and molecular diagnosis of cysticercosis. Pathog Glob Health 106: 286–298.
-
6.
Garcia HH , Rodriguez S , Gilman RH , Gonzalez AE , Tsang VC , 2012. Neurocysticercosis: is serology useful in the absence of brain imaging? Trop Med Int Health 17: 1014–1018.
-
7.
Castillo Y et al., 2009. Urine antigen detection for the diagnosis of human neurocysticercosis. Am J Trop Med Hyg 80: 379–383.
-
8.
Mwape KE , Praet N , Benitez-Ortiz W , Muma JB , Zulu G , Celi-Erazo M , Phiri IK , Rodriguez-Hidalgo R , Dorny P , Gabriël S , 2011. Field evaluation of urine antigen detection for diagnosis of Taenia solium cysticercosis. Trans R Soc Trop Med Hyg 105: 574–578.
-
9.
McCleery E et al., 2020. Population screening for urine antigens to detect asymptomatic subarachnoid neurocysticercosis: a pilot study in northern Peru. Am J Trop Med Hyg 103: 1125–1128.
-
10.
Toribio L , Romano M , Scott AL , Gonzales I , Saavedra H , Garcia HH , Shiff C ; For The Cysticercosis Working Group In Peru , 2019. Detection of Taenia solium DNA in the urine of neurocysticercosis patients. Am J Trop Med Hyg 100: 327–329.
-
11.
Abanto J , Blanco D , Saavedra H , Gonzales I , Siu D , Pretell EJ , Bustos JA , Garcia HH ; Cysticercosis Working Group in Peru , 2021. Mortality in parenchymal and subarachnoid neurocysticercosis. Am J Trop Med Hyg 105: 176–180.
-
12.
Paredes A et al., 2016. Anti-Taenia solium monoclonal antibodies for the detection of parasite antigens in body fluids from patients with neurocysticercosis. Exp Parasitol 166: 37–43.
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A Rapid Point-of-Care Assay for Cysticercosis Antigen Detection in Urine Samples
Luz Toribio
Luz Toribio
Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru;
Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;
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Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;
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Sukwan Handali
Sukwan Handali
Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia;
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Yazmin Marin
Yazmin Marin
Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;
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Erika Perez
Erika Perez
Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;
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Yesenia Castillo
Yesenia Castillo
Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru;
Parasite Immunology Laboratory, Universidad Peruana Cayetano Heredia, Lima, Peru;
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Parasite Immunology Laboratory, Universidad Peruana Cayetano Heredia, Lima, Peru;
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Javier A. Bustos
Javier A. Bustos
Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru;
Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;
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Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;
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Seth E. O’Neal
Seth E. O’Neal
School of Public Health, Oregon Health & Sciences University-Portland State University, Portland, Oregon;
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Hector H. Garcia
for The Cysticercosis Working Group in Peru
Hector H. Garcia
Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru;
Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;
Asociación Benéfica PRISMA, Lima, Peru;
Department of International Health, Johns Hopkins School of Public Health, Baltimore, Maryland
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Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;
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Department of International Health, Johns Hopkins School of Public Health, Baltimore, Maryland
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ABSTRACT.
We report a proof-of-concept study using a dipstick assay to detect Taenia solium antigen in urine samples of 30 patients with subarachnoid neurocysticercosis and 10 healthy control subjects. Strips were read in blind by two readers. The assay detected antigen in 29 of 30 cases and was negative in all 10 control samples. Although this study was performed in samples from individuals with subarachnoid neurocysticercosis who likely had high circulating antigen levels, it provides the proof of concept for a functional urine antigen point-of-care assay that detects viable cysts. Such an assay could serve to support a clinical diagnosis of suspect neurocysticercosis or to identify patients at risk of developing severe disease in areas where medical resources are limited, providing evidence to refer these individuals for imaging and specialized care as needed.
Author Notes
Authors’ addresses: Luz Toribio and Javier A. Bustos, Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru, and Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru, E-mails: luz.toribio.salazar@upch.pe and javier.bustos.p@upch.pe. Sukwan Handali, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, GA, E-mail: ahi0@cdc.gov. Yazmin Marin and Erika Perez, Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru, E-mails: yazmin.marin@upch.pe and perez.paredes.e@gmail.com. Yesenia Castillo, Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru, and Parasite Immunology Laboratory, Universidad Peruana Cayetano Heredia, Lima, Peru, E-mail: yesenia.castillo.b@upch.pe. Seth E. O’Neal, School of Public Health, Oregon Health & Sciences University-Portland State University, Portland, OR, E-mail: natseth7@gmail.com. Hector H. Garcia, Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru, Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru, Asociacion Bene fica PRISMA, Lima, Peru, and Department of International Health, Johns Hopkins School of Public Health, Baltimore, MD, E-mail: hgarcia@jhsph.edu.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Cantey PT , Coyle CM , Sorvillo FJ , Wilkins PP , Starr MC , Nash TE , 2014. Neglected parasitic infections in the United States: cysticercosis. Am J Trop Med Hyg 90: 805–809.
-
2.
Garcia HH , Gonzalez AE , Gilman RH , 2020. Taenia solium cysticercosis and its impact in neurological disease. Clin Microbiol Rev 33: e00085-19.
-
3.
White AC Jr et al., 2018. Diagnosis and treatment of neurocysticercosis: 2017 clinical practice guidelines by the Infectious Diseases Society of America (IDSA) and the American Society of Tropical Medicine and Hygiene (ASTMH). Nephrol Dial Transplant 66: 1159–1163.
-
4.
Bustos J , Gonzales I , Saavedra H , Handali S , Garcia HH , 2021. Neurocysticercosis: a frequent cause of seizures, epilepsy, and other neurological morbidity in most of the world. J Neurol Sci 427: 117527.
-
5.
Rodriguez S , Wilkins P , Dorny P , 2012. Immunological and molecular diagnosis of cysticercosis. Pathog Glob Health 106: 286–298.
-
6.
Garcia HH , Rodriguez S , Gilman RH , Gonzalez AE , Tsang VC , 2012. Neurocysticercosis: is serology useful in the absence of brain imaging? Trop Med Int Health 17: 1014–1018.
-
7.
Castillo Y et al., 2009. Urine antigen detection for the diagnosis of human neurocysticercosis. Am J Trop Med Hyg 80: 379–383.
-
8.
Mwape KE , Praet N , Benitez-Ortiz W , Muma JB , Zulu G , Celi-Erazo M , Phiri IK , Rodriguez-Hidalgo R , Dorny P , Gabriël S , 2011. Field evaluation of urine antigen detection for diagnosis of Taenia solium cysticercosis. Trans R Soc Trop Med Hyg 105: 574–578.
-
9.
McCleery E et al., 2020. Population screening for urine antigens to detect asymptomatic subarachnoid neurocysticercosis: a pilot study in northern Peru. Am J Trop Med Hyg 103: 1125–1128.
-
10.
Toribio L , Romano M , Scott AL , Gonzales I , Saavedra H , Garcia HH , Shiff C ; For The Cysticercosis Working Group In Peru , 2019. Detection of Taenia solium DNA in the urine of neurocysticercosis patients. Am J Trop Med Hyg 100: 327–329.
-
11.
Abanto J , Blanco D , Saavedra H , Gonzales I , Siu D , Pretell EJ , Bustos JA , Garcia HH ; Cysticercosis Working Group in Peru , 2021. Mortality in parenchymal and subarachnoid neurocysticercosis. Am J Trop Med Hyg 105: 176–180.
-
12.
Paredes A et al., 2016. Anti-Taenia solium monoclonal antibodies for the detection of parasite antigens in body fluids from patients with neurocysticercosis. Exp Parasitol 166: 37–43.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 3018 | 1755 | 127 |
| Full Text Views | 326 | 129 | 0 |
| PDF Downloads | 248 | 40 | 0 |