A Rapid Point-of-Care Assay for Cysticercosis Antigen Detection in Urine Samples

Luz Toribio Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru;
Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;

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Sukwan Handali Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia;

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Yazmin Marin Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;

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Erika Perez Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;

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Yesenia Castillo Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru;
Parasite Immunology Laboratory, Universidad Peruana Cayetano Heredia, Lima, Peru;

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Javier A. Bustos Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru;
Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;

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Seth E. O’Neal School of Public Health, Oregon Health & Sciences University-Portland State University, Portland, Oregon;

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Hector H. Garcia Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru;
Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;
Asociación Benéfica PRISMA, Lima, Peru;
Department of International Health, Johns Hopkins School of Public Health, Baltimore, Maryland

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for The Cysticercosis Working Group in Peru
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ABSTRACT.

We report a proof-of-concept study using a dipstick assay to detect Taenia solium antigen in urine samples of 30 patients with subarachnoid neurocysticercosis and 10 healthy control subjects. Strips were read in blind by two readers. The assay detected antigen in 29 of 30 cases and was negative in all 10 control samples. Although this study was performed in samples from individuals with subarachnoid neurocysticercosis who likely had high circulating antigen levels, it provides the proof of concept for a functional urine antigen point-of-care assay that detects viable cysts. Such an assay could serve to support a clinical diagnosis of suspect neurocysticercosis or to identify patients at risk of developing severe disease in areas where medical resources are limited, providing evidence to refer these individuals for imaging and specialized care as needed.

Author Notes

Address correspondence to Luz Toribio, Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru. E-mail: luz.toribio.salazar@upch.pe

Authors’ addresses: Luz Toribio and Javier A. Bustos, Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru, and Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru, E-mails: luz.toribio.salazar@upch.pe and javier.bustos.p@upch.pe. Sukwan Handali, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, GA, E-mail: ahi0@cdc.gov. Yazmin Marin and Erika Perez, Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru, E-mails: yazmin.marin@upch.pe and perez.paredes.e@gmail.com. Yesenia Castillo, Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru, and Parasite Immunology Laboratory, Universidad Peruana Cayetano Heredia, Lima, Peru, E-mail: yesenia.castillo.b@upch.pe. Seth E. O’Neal, School of Public Health, Oregon Health & Sciences University-Portland State University, Portland, OR, E-mail: natseth7@gmail.com. Hector H. Garcia, Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru, Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru, Asociacion Bene fica PRISMA, Lima, Peru, and Department of International Health, Johns Hopkins School of Public Health, Baltimore, MD, E-mail: hgarcia@jhsph.edu.

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