Infection of colonized cat fleas, Ctenocephalides felis (Bouche), with a Rickettsia-like microorganism. Am J Trop Med Hyg43: 400–409.10.4269/ajtmh.1990.43.400)| false
Identification of a novel rickettsial infection in a patient diagnosed with murine typhus. J Clin Microbiol32: 949–954.10.1128/jcm.32.4.949-954.1994)| false
WilliamsSG, SacciJB, SchrieferME, AndersenEM, FujiokaKK, SorvilloFJ, BarrAR, AzadAF
Typhus and typhus-like rickettsiae associated with opossums and their fleas in Los Angeles County, California. J Clin Microbiol30: 1758–1762.
Typhus and typhus-like rickettsiae associated with opossums and their fleas in Los Angeles County, California. J Clin Microbiol30: 1758–1762.10.1128/jcm.30.7.1758-1762.1992)| false
MullinsKE, MainaAN, KruegerL, JiangJ, CummingsR, DrusysA, WilliamsG, DhillonM, RichardsAL
Rickettsial infections among cats and cat fleas in Riverside County, California. Am J Trop Med Hyg99: 291–296.
Rickettsial infections among cats and cat fleas in Riverside County, California. Am J Trop Med Hyg99: 291–296.10.4269/ajtmh.17-0706)| false
Geographic association of Rickettsia felis-infected opossums with human murine typhus, Texas. Emerg Infect Dis8: 549–554.10.3201/eid0806.010350)| false
Opossums and cat fleas: new insights in the ecology of murine typhus in Galveston, Texas. Am J Trop Med Hyg95: 457–461.10.4269/ajtmh.16-0197)| false
Rickettsia felis infection acquired in Europe and documented by polymerase chain reaction. Emerg Infect Dis8: 207–208.10.3201/eid0802.010293)| false
OteoJA, PortilloA, SantibáñezS, BlancoJR, Pérez-MartínezL, IbarraV
Cluster of cases of human Rickettsia felis infection from southern Europe (Spain) diagnosed by PCR. J Clin Microbiol44: 2669–2671.
Cluster of cases of human Rickettsia felis infection from southern Europe (Spain) diagnosed by PCR. J Clin Microbiol44: 2669–2671.10.1128/JCM.00366-06)| false
Taxonomic study of the genus Ctenocephalides Stiles & Collins, 1930 (Insecta: Siphonaptera: Pulicidae) by using aedeagus characters. J Med Entomol35: 883–890.10.1093/jmedent/35.5.883)| false
Ctenocephalides felis felis vs. Ctenocephalides canis (Siphonaptera: Pulicidae): some issues in correctly identify these species. Rev Bras Parasitol Vet21: 345–354.10.1590/S1984-29612012000400002)| false
LabrunaMB, WhitworthT, HortaMC, BouyerDH, McBrideJW, PinterA, PopovV, GennariSM, WalkerDH
Rickettsia species infecting Amblyomma cooperi ticks from an area in the state of São Paulo, Brazil, where Brazilian spotted fever is endemic. J Clin Microbiol42: 90–98.
Rickettsia species infecting Amblyomma cooperi ticks from an area in the state of São Paulo, Brazil, where Brazilian spotted fever is endemic. J Clin Microbiol42: 90–98.10.1128/JCM.42.1.90-98.2004)| false
Phylogenetic analysis of members of the genus Rickettsia using the gene encoding the outer-membrane protein rOmpB (ompB). Int J Syst Evol Microbiol50: 1449–1455.10.1099/00207713-50-4-1449)| false
Serological evidence of exposure to Rickettsia felis and Rickettsia typhi in Australian veterinarians. Parasit Vectors10: 129.10.1186/s13071-017-2075-y)| false
Flea-borne spotted fever is an emerging insect-borne rickettsial infection caused by Rickettsia felis and has been identified worldwide. This study sought to explore the prevalence of rickettsiae associated with fleas on companion dogs and cats from Walker and Montgomery Counties in East Texas. Fleas were collected from animals entering local veterinary clinics for routine checkups. Collected fleas were identified as Ctenocephalides felis or Pulex irritans and analyzed by polymerase chain reaction for the presence of rickettsiae and subsequent sequencing. An estimation of the bcMLE (bias-corrected maximum likelihood estimation) of pooled samples was calculated. Four hundred eighty-eight fleas (comprising C. felis and P. irritans) were collected from 16 cats and 77 dogs. Our results demonstrate R. felis in 21 pools of fleas from dogs (bcMLE 15.28%) and a bcMLE of 7.25% from flea samples collected from cats. Sequence analysis revealed R. felis as the only Rickettsia that could be amplified in our samples using the rickettsial citrate synthase gene and subsequent sequencing. In this study, the presence of R. felis in fleas from companion cats and dogs suggests a potential risk of flea-borne spotted fever in humans who encounter flea-infested animals.
Address correspondence to Jeremy Bechelli, Department of Biological Sciences, College of Science and Engineering Technology, Sam Houston State University, 2000 Ave I, Life Sciences Building, Huntsville, TX 77341-2116. E-mail: email@example.com