Authors:
, , , , , , , , , , , , , , , , , and
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Garcia HH, Gonzalez AE, Gilman RH , 2020. Taenia solium cysticercosis and its impact in neurological disease. Clin Microbiol Rev 33: e00085–19.
-
2.
Del Brutto OH, Nash TE, White AC, Rajshekhar V, Wilkins PP, Singh G, Vasquez CM, Salgado P, Gilman RH, Garcia HH , 2017. Revised diagnostic criteria for neurocysticercosis. J Neurol Sci 372: 202–210.
-
3.
Garcia HH, Rodriguez S, Gilman RH, Gonzalez AE, Tsang VCW , for the Cysticercosis Working Group in Peru , 2012. Neurocysticercosis: is serology useful in the absence of brain imaging? Trop Med Int Health 17: 1014–1018.
-
4.
McCleery E, Allen SE, Moyano LM, Gamboa R, Vilchez P, Muro C, Castillo Y, Dorny P, Garcia HH, O’Neal SE , for the Cysticercosis Working Group in Peru , 2020. Population screening for urine antigens to detect asymptomatic subarachnoid neurocysticercosis: a pilot study in northern Peru. Am J Trop Med Hyg 103: 1125.
-
5.
Castillo Y et al.Cysticercosis Working Group in Perú , 2009. Urine antigen detection for the diagnosis of human neurocysticercosis. Am J Trop Med Hyg 80: 379–383.
-
6.
Toribio L, Romano M, Scott AL, Gonzales I, Saavedra H, Garcia HH, Shiff C , for the Cysticercosis Working Group in Peru , 2019. Detection of Taenia solium DNA in the urine of neurocysticercosis patients. Am J Trop Med Hyg 100: 327–329.
-
7.
Toribio L, Santivanez S, Scott AL, Enriquez R, Sedano C, Soto-Becerra P, Garcia HH, Shiff CJ , 2020. Diagnostic urinary cfDNA detected in human cystic echinococcosis. Mol Biochem Parasitol 239: 111314.
-
8.
Beckonert O, Keun HC, Ebbels TMD, Bundy J, Holmes E, Lindon JC, Nicholson JK , 2007. Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts. Nat Protoc 2: 2692–2703.
-
9.
Aguilar JA, Nilsson M, Bodenhausen G, Morris GA , 2011. Spin echo NMR spectra without J modulation. Chem Commun (Camb) 48: 811–813.
-
10.
Savorani F, Tomasi G, Engelsen SB, 2013. Alignment of 1D NMR Data using the iCoshift tool: a tutorial. Van Duynhoven J, Belton PS, Webb GA, van As H, eds. Magnetic Resonance in Food Science. London, UK: Royal Society of Chemistry, 14–24.
-
11.
Gebregiworgis T, Powers R , 2012. Application of NMR metabolomics to search for human disease biomarkers. Comb Chem High Throughput Screen 15: 595–610.
-
12.
Shin J-H, Yang J-Y, Jeon B-Y, Yoon YJ, Cho S-N, Kang Y-H, Ryu DH, Hwang G-S , 2011. (1)H NMR-based metabolomic profiling in mice infected with Mycobacterium tuberculosis. J Proteome Res 10: 2238–2247.
-
13.
Teng R, Junankar PR, Bubb WA, Rae C, Mercier P, Kirk K , 2009. Metabolite profiling of the intraerythrocytic malaria parasite Plasmodium falciparum by (1)H NMR spectroscopy. NMR Biomed 22: 292–302.
-
14.
Slupsky CM , 2010. NMR-based analysis of metabolites in urine provides rapid diagnosis and etiology of pneumonia. Biomarkers Med 4: 195–197.
-
15.
DeFeo EM, Wu C-L, McDougal WS, Cheng LL , 2011. A decade in prostate cancer: from NMR to metabolomics. Nat Rev Urol 8: 301–311.
-
16.
Beger RD , 2013. A review of applications of metabolomics in cancer. Metabolites 3: 552–574.
-
17.
Ranjan R, Sinha N , 2019. Nuclear magnetic resonance (NMR)-based metabolomics for cancer research. NMR Biomed 32: e3916.
-
18.
Ahmed SS, Santosh W, Kumar S, Christlet HTT , 2009. Metabolic profiling of Parkinson’s disease: evidence of biomarker from gene expression analysis and rapid neural network detection. J Biomed Sci 16: 63.
-
19.
Sinclair AJ, Viant MR, Ball AK, Burdon MA, Walker EA, Stewart PM, Rauz S, Young SP , 2010. NMR-based metabolomic analysis of cerebrospinal fluid and serum in neurological diseases—a diagnostic tool? NMR Biomed 23: 123–132.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 6346 | 1872 | 325 |
| Full Text Views | 278 | 17 | 3 |
| PDF Downloads | 110 | 20 | 4 |
Urine 1H-NMR Metabolomics to Discriminate Neurocysticercosis Patients from Healthy Controls: An Exploratory Study
Juan M. Lopez
Juan M. Lopez
Departamento de Ciencias—Química, CERMN, Pontificia Universidad Católica del Perú, Lima, Perú;
Search for other papers by Juan M. Lopez in
Current site
Google Scholar
PubMed
Search for other papers by Juan M. Lopez in
Current site
Google Scholar
PubMed
Vanessa E. Leyva
Vanessa E. Leyva
Departamento de Ciencias—Química, CERMN, Pontificia Universidad Católica del Perú, Lima, Perú;
Search for other papers by Vanessa E. Leyva in
Current site
Google Scholar
PubMed
Search for other papers by Vanessa E. Leyva in
Current site
Google Scholar
PubMed
Javier A. Bustos
Javier A. Bustos
Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Perú;
Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Perú
Search for other papers by Javier A. Bustos in
Current site
Google Scholar
PubMed
Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Perú
Search for other papers by Javier A. Bustos in
Current site
Google Scholar
PubMed
Erika Perez
Erika Perez
Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Perú;
Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Perú
Search for other papers by Erika Perez in
Current site
Google Scholar
PubMed
Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Perú
Search for other papers by Erika Perez in
Current site
Google Scholar
PubMed
Sofía S. Sanchez
Sofía S. Sanchez
Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Perú
Search for other papers by Sofía S. Sanchez in
Current site
Google Scholar
PubMed
Search for other papers by Sofía S. Sanchez in
Current site
Google Scholar
PubMed
Herbert Saavedra
Herbert Saavedra
Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Perú
Search for other papers by Herbert Saavedra in
Current site
Google Scholar
PubMed
Search for other papers by Herbert Saavedra in
Current site
Google Scholar
PubMed
Isidro Gonzales
Isidro Gonzales
Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Perú
Search for other papers by Isidro Gonzales in
Current site
Google Scholar
PubMed
Search for other papers by Isidro Gonzales in
Current site
Google Scholar
PubMed
Helena Maruenda
Helena Maruenda
Departamento de Ciencias—Química, CERMN, Pontificia Universidad Católica del Perú, Lima, Perú;
Search for other papers by Helena Maruenda in
Current site
Google Scholar
PubMed
Search for other papers by Helena Maruenda in
Current site
Google Scholar
PubMed
Hector H. Garcia
Hector H. Garcia
Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Perú;
Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Perú
Search for other papers by Hector H. Garcia in
Current site
Google Scholar
PubMed
Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Perú
Search for other papers by Hector H. Garcia in
Current site
Google Scholar
PubMed
for the Cysticercosis Working Group in Peru
for the Cysticercosis Working Group in Peru
Search for other papers by for the Cysticercosis Working Group in Peru in
Current site
Google Scholar
PubMed
Search for other papers by for the Cysticercosis Working Group in Peru in
Current site
Google Scholar
PubMed
ABSTRACT.
The diagnosis of neurocysticercosis (NCC) is principally based on neuroimaging (magnetic resonance imaging or computed tomography), instrumentation that is scarcely available in the rural regions where Taenia solium transmission, primarily occurs due to poor sanitation conditions. Immunological assays for antigen or antibody detection complement the neuroimaging approach. However, no field-applicable assays to diagnose viable NCC or to guide the referral of cases for neuroimaging or for appropriate management are available. We performed an exploratory study on urine and serum samples using 1H-nuclear magnetic resonance (NMR)-based metabolomics to discriminate NCC patients (n = 14) from healthy control subjects (n = 22). Metabolic profiles demonstrated a discrimination between the urines of NCC patients and noninfected control subjects with a moderate predictive accuracy (R2 = 0.999, Q2 = 0.434). NMR metabolomics analysis has been proven useful in depicting biomarkers linked to other infectious diseases, various types of cancer, and other disorders. Our results, albeit preliminary, open a door to the development of better methods for detecting NCC through the identification of biomarkers participating in disturbed metabolic pathways.
- Supplemental Materials (PDF 820 KB)
Author Notes
Authors’ addresses: Juan M. Lopez, Vanessa E. Leyva, and Helena Maruenda, Departamento de Ciencias—Química, CERMN, Pontificia Universidad Católica del Perú, Lima, Perú, E-mails: juan.lopez@pucp.edu.pe, vleyva@pucp.edu.pe, and hmaruen@pucp.edu.pe. Javier A. Bustos, Universidad Peruana Cayetano Heredia, Centro de Salud Global, Lima, Perú, E-mail: javier.bustos.p@upch.pe. Erika Perez, Sofía Sánchez, and Herbert Saavedra, Instituto Nacional De Ciencias Neurologicas, Unidad de Cisticercosis, Lima, Perú, E-mails: perez.paredes.e@gmail.com, sssboluarte@gmail.com, and hsaavedrapastor@hotmail.com. Isidro Gonzales, Instituto Nacional de Ciencias Neurológicas, Lima, Perú, and Unidad de Investigación/Centro Básico de Investigación en Exámenes Auxiliares en Parasitosis del Sistema Nervioso Central, Instituto Nacional de Ciencias Neurológicas, Lima, Perú, E-mail: isidrogonzalesq@hotmail.com. Hector H. Garcia, Universidad Peruana Cayetano Heredia, Microbiology, Lima, Perú, and Instituto de Ciencias Neurologicas, Transmissible Diseases, Lima, Perú, E-mail: hgarcia@jhsph.edu.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Garcia HH, Gonzalez AE, Gilman RH , 2020. Taenia solium cysticercosis and its impact in neurological disease. Clin Microbiol Rev 33: e00085–19.
-
2.
Del Brutto OH, Nash TE, White AC, Rajshekhar V, Wilkins PP, Singh G, Vasquez CM, Salgado P, Gilman RH, Garcia HH , 2017. Revised diagnostic criteria for neurocysticercosis. J Neurol Sci 372: 202–210.
-
3.
Garcia HH, Rodriguez S, Gilman RH, Gonzalez AE, Tsang VCW , for the Cysticercosis Working Group in Peru , 2012. Neurocysticercosis: is serology useful in the absence of brain imaging? Trop Med Int Health 17: 1014–1018.
-
4.
McCleery E, Allen SE, Moyano LM, Gamboa R, Vilchez P, Muro C, Castillo Y, Dorny P, Garcia HH, O’Neal SE , for the Cysticercosis Working Group in Peru , 2020. Population screening for urine antigens to detect asymptomatic subarachnoid neurocysticercosis: a pilot study in northern Peru. Am J Trop Med Hyg 103: 1125.
-
5.
Castillo Y et al.Cysticercosis Working Group in Perú , 2009. Urine antigen detection for the diagnosis of human neurocysticercosis. Am J Trop Med Hyg 80: 379–383.
-
6.
Toribio L, Romano M, Scott AL, Gonzales I, Saavedra H, Garcia HH, Shiff C , for the Cysticercosis Working Group in Peru , 2019. Detection of Taenia solium DNA in the urine of neurocysticercosis patients. Am J Trop Med Hyg 100: 327–329.
-
7.
Toribio L, Santivanez S, Scott AL, Enriquez R, Sedano C, Soto-Becerra P, Garcia HH, Shiff CJ , 2020. Diagnostic urinary cfDNA detected in human cystic echinococcosis. Mol Biochem Parasitol 239: 111314.
-
8.
Beckonert O, Keun HC, Ebbels TMD, Bundy J, Holmes E, Lindon JC, Nicholson JK , 2007. Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts. Nat Protoc 2: 2692–2703.
-
9.
Aguilar JA, Nilsson M, Bodenhausen G, Morris GA , 2011. Spin echo NMR spectra without J modulation. Chem Commun (Camb) 48: 811–813.
-
10.
Savorani F, Tomasi G, Engelsen SB, 2013. Alignment of 1D NMR Data using the iCoshift tool: a tutorial. Van Duynhoven J, Belton PS, Webb GA, van As H, eds. Magnetic Resonance in Food Science. London, UK: Royal Society of Chemistry, 14–24.
-
11.
Gebregiworgis T, Powers R , 2012. Application of NMR metabolomics to search for human disease biomarkers. Comb Chem High Throughput Screen 15: 595–610.
-
12.
Shin J-H, Yang J-Y, Jeon B-Y, Yoon YJ, Cho S-N, Kang Y-H, Ryu DH, Hwang G-S , 2011. (1)H NMR-based metabolomic profiling in mice infected with Mycobacterium tuberculosis. J Proteome Res 10: 2238–2247.
-
13.
Teng R, Junankar PR, Bubb WA, Rae C, Mercier P, Kirk K , 2009. Metabolite profiling of the intraerythrocytic malaria parasite Plasmodium falciparum by (1)H NMR spectroscopy. NMR Biomed 22: 292–302.
-
14.
Slupsky CM , 2010. NMR-based analysis of metabolites in urine provides rapid diagnosis and etiology of pneumonia. Biomarkers Med 4: 195–197.
-
15.
DeFeo EM, Wu C-L, McDougal WS, Cheng LL , 2011. A decade in prostate cancer: from NMR to metabolomics. Nat Rev Urol 8: 301–311.
-
16.
Beger RD , 2013. A review of applications of metabolomics in cancer. Metabolites 3: 552–574.
-
17.
Ranjan R, Sinha N , 2019. Nuclear magnetic resonance (NMR)-based metabolomics for cancer research. NMR Biomed 32: e3916.
-
18.
Ahmed SS, Santosh W, Kumar S, Christlet HTT , 2009. Metabolic profiling of Parkinson’s disease: evidence of biomarker from gene expression analysis and rapid neural network detection. J Biomed Sci 16: 63.
-
19.
Sinclair AJ, Viant MR, Ball AK, Burdon MA, Walker EA, Stewart PM, Rauz S, Young SP , 2010. NMR-based metabolomic analysis of cerebrospinal fluid and serum in neurological diseases—a diagnostic tool? NMR Biomed 23: 123–132.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 6346 | 1872 | 325 |
| Full Text Views | 278 | 17 | 3 |
| PDF Downloads | 110 | 20 | 4 |