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First Record of Natural Transovarial Transmission of Dengue Virus in Aedes albopictus from Cuba

Luis Augusto PiedraVector Control Department, Institute of Tropical Medicine “Pedro Kourí,” Havana, Cuba;

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Liss Claudia MartinezVector Control Department, Institute of Tropical Medicine “Pedro Kourí,” Havana, Cuba;

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Armando RuizVector Control Department, Institute of Tropical Medicine “Pedro Kourí,” Havana, Cuba;

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Juan Ramon VazquezFaculty of Health Sciences, Havana, Cuba;

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Maria Guadalupe GuzmanDepartment of Virology, Institute of Tropical Medicine “Pedro Kourí,” Havana, Cuba;

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Jorge ReyFlorida Medical Entomology Laboratory, University of Florida, Gainesville, Florida

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Juan Andres BissetVector Control Department, Institute of Tropical Medicine “Pedro Kourí,” Havana, Cuba;

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ABSTRACT.

Transovarial transmission (TOT) of dengue virus (DENV) in Aedes spp. is an important mechanism for DENV maintenance in nature and may be important in initiating outbreaks. The objective of this study was to explore the occurrence of TOT in wild Aedes albopictus populations in Cuba. Mosquito larvae were collected in Cotorro municipality, Havana, Cuba, and identified to species. Fifteen pools of Ae. albopictus each containing 30 larvae were processed for DENV detection by using conventional reverse transcription polymerase chain reaction (RT-PCR) and nested PCR. Four out of 15 pools processed were positive for DENV-3, but no other DENV serotype was detected. This is the first time TOT of DENV detected in Cuban field populations of Ae. albopictus, and this suggests that this species may be an important vector of DENV in Cuba.

Author Notes

Address correspondence to Luis Augusto Piedra, Vector Control Department, Institute of Tropical Medicine “Pedro Kourí,” Avenida Novia del Mediodia, KM 6 1/2, La Lisa, La Habana, 11400, Cuba. E-mail: piedra@ipk.sld.cu

Authors’ addresses: Luis Augusto Piedra, Liss Claudia Martinez, Armando Ruiz, and Juan Andres Bisset, Vector Control Department, Institute of Tropical Medicine “Pedro Kourí,” Havana, Cuba, E-mails: piedra@ipk.sld.cu, liss.martinez@ipk.sld.cu, amruiz@ipk.sld.cu, and bisset@ipk.sld.cu. Juan Ramon Vazquez, Faculty of Health Sciences, Havana, Cuba, E-mail: juanr.cangas@infomed.sld.cu. Maria Guadalupe Guzman, Department of Virology, Institute of Tropical Medicine “Pedro Kourí,” Havana, Cuba, E-mail: lupe@ipk.sld.cu. Jorge Rey, Florida Medical Entomology Laboratory, University of Florida, Gainesville, FL, E-mail: jrey@ufl.edu.

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