• 1.

    Griffith DE et al.2007. An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. Am J Respir Crit Care Med 175: 367416.

    • Search Google Scholar
    • Export Citation
  • 2.

    Sharma S, Sharma R, Singh B, Upadhyay V, Mani I, Tripathi M & Kumar P 2019. A prospective study of non-tuberculous mycobacterial disease among tuberculosis suspects at a tertiary care centre in north India. Indian J Med Res 150: 458467.

    • Search Google Scholar
    • Export Citation
  • 3.

    Piersimoni C & Scarparo C 2009. Extrapulmonary infections associated with nontuberculous mycobacteria in immunocompetent persons. Emerg Infect Dis 15: 13511358.

    • Search Google Scholar
    • Export Citation
  • 4.

    Jani MN, Rodrigues CS & Mehta AP 2011. The neglected and often ignored: nontuberculous mycobacteria. J Glob Infect Dis 3: 94.

  • 5.

    Park H, Jang H, Song E, Chang CL, Lee M, Jeong S, Park J, Kang B & Kim C 2005. Detection and genotyping of Mycobacterium species from clinical isolates and specimens by oligonucleotide array. J Clin Microbiol 43: 17821788.

    • Search Google Scholar
    • Export Citation
  • 6.

    Suresh P, Biswas L, Prasad V, Kumar A, Sivadas S, Khan S & Biswas R 2020. BCG infection due to MPT64-negative strain: a diagnostic challenge. Am J Trop Med Hyg 103: 10721075.

    • Search Google Scholar
    • Export Citation
  • 7.

    Shenai S, Rodrigues C & Mehta A 2010. Time to identify and define non-tuberculous mycobacteria in a tuberculosis-endemic region. Int J Tuberc Lung Dis 14: 10011008.

    • Search Google Scholar
    • Export Citation
  • 8.

    Sharma P, Singh D, Sharma K, Verma S, Mahajan S & Kanga A 2018. Are we neglecting nontuberculous mycobacteria just as laboratory contaminants? Time to reevaluate things. J Pathogens 8907629. doi: 10.1155/2018/8907629.

    • Search Google Scholar
    • Export Citation
  • 9.

    Myneedu VP, Verma AK, Bhalla M, Arora J, Reza S, Sah GC & Behera D 2013. Occurrence of non-tuberculous Mycobacterium in clinical samples—a potential pathogen. Indian J Tuberc 60: 7176.

    • Search Google Scholar
    • Export Citation
  • 10.

    Umrao J, Singh D, Zia A, Saxena S, Sarsaiya S, Singh S, Khatoon J & Dhole TN 2016. Prevalence and species spectrum of both pulmonary and extrapulmonary nontuberculous mycobacteria isolates at a tertiary care center. Int J Mycobacteriol 5: 288293.

    • Search Google Scholar
    • Export Citation
  • 11.

    Jain S, Sankar MM, Sharma N, Singh S & Chugh TD 2014. High prevalence of non-tuberculous mycobacterial disease among non-HIV infected individuals in a TB endemic country—experience from a tertiary center in Delhi, India. Pathog Glob Health 108: 118122.

    • Search Google Scholar
    • Export Citation
  • 12.

    Gopinath K & Singh S 2010. Non-tuberculous mycobacteria in TB-endemic countries: are we neglecting the danger? PLoS Negl Trop Dis 4: e615.

  • 13.

    Maurya AK, Nag VL, Kant S, Kushwaha RAS, Kumar M, Singh AK & Dhole TN 2015. Prevalence of nontuberculous mycobacteria among extrapulmonary tuberculosis cases in tertiary care centers in northern India. BioMed Res Int 465403. doi: 10.1155/2015/465403.

    • Search Google Scholar
    • Export Citation
  • 14.

    Renaud CJ, Subramanian S, Tambyah PA & Lee EJC 2011. The clinical course of rapidly growing nontuberculous mycobacterial peritoneal dialysis infections in Asians: a case series and literature review. Nephrology (Carlton) 16: 174179.

    • Search Google Scholar
    • Export Citation
  • 15.

    Patil R, Patil T, Schenfeld L & Massoud S 2011. Mycobacterium porcinum peritonitis in a patient on continuous ambulatory peritoneal dialysis. J Gen Intern Med 26: 346348.

    • Search Google Scholar
    • Export Citation
  • 16.

    Spaulding AB, Lai YL, Zelazny AM, Olivier KN, Kadri SS, Prevots DR & Adjemian J 2017. Geographic distribution of nontuberculous mycobacterial species identified among clinical isolates in the United States, 2009–2013. Ann Am Thorac Soc 14: 16551661.

    • Search Google Scholar
    • Export Citation
 
 
 
 

 

 
 
 

 

 

 

 

 

 

Epidemiology of Nontuberculous Mycobacterial Infection in Tuberculosis Suspects

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  • 1 Center for Nanosciences and Molecular medicine, Amrita Vishwa Vidyapeetham, Ponekkara, Kochi, Kerala, India;
  • | 2 Department of Microbiology, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Ponekkara, Kochi, Kerala, India;
  • | 3 Department of Dermatology, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Ponekkara, Kochi, Kerala, India;
  • | 4 Department of Respiratory Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Ponekkara, Kochi, Kerala, India

ABSTRACT.

Nontuberculosis mycobacteria (NTM) are opportunistic pathogens that cause a wide range of illnesses. Here, the species distribution and prevalence of NTM infections in tuberculosis suspects was analyzed. A total of 7,073 specimens from pulmonary and extrapulmonary sites were analyzed, and 709 (10%) were found to be culture positive for mycobacteria. Of these, 85.2% were identified as Mycobacterium tuberculosis complex and 14.8% as NTM (65.7% rapid growers and 34.3% slow growers). Speciation of the NTM isolates (n = 69) identified 19 NTM species. M. abscessus (33.3%) and M. fortuitum (24.6%) were the most dominant NTM species isolated from the patients, followed by M. porcinum (5.8%) and M. parascrofulaceum (4.3%). We also report peritonitis caused by rapidly growing NTM among the patients undergoing continuous ambulatory peritoneal dialysis and a case of M. senegalense peritonitis. A low prevalence but high species diversity of NTM was detected in our study. The high species diversity of NTM necessitates the need to unequivocally identify mycobacterial isolates for appropriate treatment.

Author Notes

Address correspondence to Anil Kumar, Department of Microbiology, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Ponekkara, Kochi-682041, Kerala, India, E-mail: vanilkumar@aims.amrita.edu or Lalitha Biswas, Center for Nanosciences and Molecular medicine, Amrita Vishwa Vidyapeetham, Ponekkara, Kochi-682041, Kerala, India, E-mail: lalithabiswas@aims.amrita.edu.

Authors’ addresses: Parasmal Suresh, Center for Nanosciences and Molecular Medicine, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India, E-mail: parassureshjn@gmail.com. Anil Kumar, Microbiology, Amrita Institute of Medical Sciences, Ponekara, Kochi, Kerala, India, E-mail: vanilkumar@aims.amrita.edu. Raja Biswas and Lalitha Biswas, Center for Nanosciences and Molecular Medicine, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India, E-mails: rajabiswas@aims.amrita.edu and lalithabiswas@aims.amrita.edu. Gopikrishnan Anjaneyan, Department of Dermatology, Amrita Institute of Medical Sciences, Kochi, Kerala, India, E-mail: gopi19430@aims.amrita.edu. Divya Vijayakumar and Swathy Thulasidharan, Department of Microbiology, Amrita Institute of Medical Sciences and Research Centre, Kochi, Kerala, India, E-mails: divarchives@gmail.com and swathy.thulasidharan@gmail.com. Akhilesh Kunoor, Department of Respiratory Medicine, Amrita Institute of Medical Sciences and Research Centre, Kochi, Kerala, India, E-mail: akhileshk@aims.amrita.edu.

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