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Serum Adsorption Study to Validate the Specificity of a Rapid Test to Detect Strongyloides stercoralis Infection

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  • 1 Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia;
  • | 2 Department of Parasitology and Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia

ABSTRACT.

A lateral flow rapid test for strongyloidiasis will greatly facilitate the control and elimination of the disease. Previously SsRapid prototype rapid test showed high diagnostic specificity to detect Strongyloides infection, determined using non-Strongyloides sera negative by IgG-ELISAs. Since high specificity is crucial before a test is used for public health control activities, further validation of its specificity is needed. Also, it needs to be ascertained whether non-Strongyloides sera positive by IgG-ELISAs and SsRapid are truly positive for Strongyloides or are cases of cross-reactivity. We performed 84 rapid tests (two types of dipsticks and cassettes) using 34 serum samples. They were divided into four groups based on Strongyloides infection and coinfection with other parasites and the availability of recombinant proteins and rapid tests for the latter. Sera was adsorbed using polystyrene microspheres beads separately coated with four recombinant parasite proteins. The small sample size is a limitation of this study; however, the overall results showed that the sera adsorption procedure was successful, and the SsRapid test is specific.

Author Notes

Address correspondence to Rahmah Noordin, Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia. E-mail: rahmah@usm.my

Financial support: This study was funded by a grant from the Malaysian Ministry of Higher Education (MOHE) MyLab grant 1/2018 (No 203.CIPPM.673014 2). We also acknowledge support from MOHE’s Institution Centre of Excellence (HICoE) program (No: 311/CIPPM/4401005).

Authors’ addresses: Rahmah Noordin, Nor Suhada Anuar, Nor Mustaiqazah Juri, Anizah Rahumatullah, and Nur Athirah Ahmad Hilmi, Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Pulau Pinang, Malaysia, E-mails: rahmah8485@gmail.com, norsuhada@usm.my, mustaiqazah@yahoo.com, anizahrahumatullah@usm.my, and hilmi.nurathirah@gmail.com. Emelia Osman, Department of Parasitology and Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia, E-mail: emelia.osman@ukm.edu.my.

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