Authors:
, , , , , , , , , , , , , , , , , and
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1.
Franco JR, Cecchi G, Priotto G, Paone M, Diarra A, Grout L, Simarro PP, Zhao W, Argaw D, 2020. Monitoring the elimination of human African trypanosomiasis at continental and country level: update to 2018. PLoS Negl Trop Dis 14: e0008261.
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2.
World Health Organization , 2020. Ending the Neglect to Attain the Sustainable Development Goals: A Road Map for Neglected Tropical Diseases 2021–2031. Geneva, Switzerland: WHO. Available at: https://www.who.int/neglected_diseases/Revised-Draft-NTD-Roadmap-23Apr2020.pdf?ua=1. Accessed March 15, 2021.
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3.
Jamonneau V et al., 2012. Untreated human infections by Trypanosoma brucei gambiense are not 100% fatal. PLoS Negl Trop Dis 6: e1691.
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4.
Nzou SM, Fujii Y, Miura M, Mwau M, Mwangi AW, Itoh M, Salam MA, Hamano S, Hirayama K, Kaneko S, 2016. Development of multiplex serological assay for the detection of human African trypanosomiasis. Parasitol Int 65: 121–127.
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5.
Arnold BF, Scobie HM, Priest JW, Lammie PJ, 2018. Integrated serologic surveillance of population immunity and disease transmission. Emerg Infect Dis 24: 1188–1194.
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6.
Lammie PJ, Moss DM, Brook Goodhew E, Hamlin K, Krolewiecki A, West SK, Priest JW, 2012. Development of a new platform for neglected tropical disease surveillance. Int J Parasitol 42: 797–800.
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7.
Sternberg JM, Gierlinski M, Bieler S, Ferguson MA, Ndung’u JM, 2014. Evaluation of the diagnostic accuracy of prototype rapid tests for human African trypanosomiasis. PLoS Negl Trop Dis 8: e3373.
-
8.
Sullivan L, Fleming J, Sastry L, Mehlert A, Wall SJ, Ferguson MA, 2014. Identification of sVSG117 as an immunodiagnostic antigen and evaluation of a dual-antigen lateral flow test for the diagnosis of human African trypanosomiasis. PLoS Negl Trop Dis 8: e2976.
-
9.
Sullivan L, Wall SJ, Carrington M, Ferguson MA, 2013. Proteomic selection of immunodiagnostic antigens for human African trypanosomiasis and generation of a prototype lateral flow immunodiagnostic device. PLoS Negl Trop Dis 7: e2087.
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10.
Priest JW, Moss DM, 2020. Measuring cryptosporidium serologic responses by multiplex bead assay. Methods Mol Biol 2052: 61–85.
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11.
Franco JR, Simarro PP, Diarra A, Ruiz-Postigo JA, Jannin JG, 2012. The human African trypanosomiasis specimen biobank: a necessary tool to support research of new diagnostics. PLoS Negl Trop Dis 6: e1571.
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12.
Feeser KR, Cama V, Priest JW, Thiele EA, Wiegand RE, Lakwo T, Feleke SM, Cantey PT, 2017. Characterizing reactivity to Onchocerca volvulus antigens in multiplex bead assays. Am J Trop Med Hyg 97: 666–672.
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13.
Priest JW, Stuchlik O, Reed M, Soboslay P, Cama V, Roy SL, 2020. Development of a multiplex bead assay for the detection of IgG antibody responses to guinea worm. Am J Trop Med Hyg 103: 2294–2304.
-
14.
Youden WJ, 1950. Index for rating diagnostic tests. Cancer 3: 32–35.
-
15.
Zweig MH, Campbell G, 1993. Receiver-operating characteristic (ROC) plots: a fundamental evaluation tool in clinical medicine. Clin Chem 39: 561–577.
-
16.
Youngstrom EA, 2014. A primer on receiver operating characteristic analysis and diagnostic efficiency statistics for pediatric psychology: we are ready to ROC. J Pediatr Psychol 39: 204–221.
-
17.
Simarro PP, Cecchi G, Paone M, Franco JR, Diarra A, Ruiz JA, Fevre EM, Courtin F, Mattioli RC, Jannin JG, 2010. The Atlas of human African trypanosomiasis: a contribution to global mapping of neglected tropical diseases. Int J Health Geogr 9: 57.
-
18.
Rooney B, Piening T, Buscher P, Roge S, Smales CM, 2015. Expression of Trypanosoma brucei gambiense antigens in Leishmania tarentolae. Potential for use in rapid serodiagnostic tests (RDTs). PLoS Negl Trop Dis 9: e0004271.
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Multiplex Bead Assay for the Detection of Human IgG Antibody Responses to African Trypanosomes
Jeffrey W. Priest
Jeffrey W. Priest
Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia;
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Sukwan Handali
Sukwan Handali
Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia
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ABSTRACT.
The recent introduction of large-scale, population-based serologic surveys in several nations where human African trypanosomiasis (HAT) remains endemic could provide an opportunity to better map the remaining disease foci and to identify asymptomatic, seropositive individuals who are infected with the more chronic form of the parasite, Trypanosoma brucei gambiense (gHAT). We have incorporated a soluble form of variant surface glycoprotein 117 and a recombinant invariant surface glycoprotein 65.1 into a multiplex bead assay (MBA) method that is commonly used for the detection of IgG antibody responses to other neglected tropical diseases. A positive result was defined as reactivity to both antigens. MBA sensitivity and specificity for gHAT infection were 92% and 96%, respectively. Assay specificity for the acute form of disease caused by T.b. rhodesiense (rHAT) was 94%, but the sensitivity was only 63.6%. In the future, additional antigens could be incorporated into the multiplex assay to improve rHAT sensitivity.
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Author Notes
Financial support: The Division of Parasitic Diseases and Malaria and the Division of Foodborne, Waterborne, and Environmental Disease at the U.S. Centers for Disease Control and Prevention provided intramural support for this work.
Disclaimer: Use of trade names is for identification only and does not imply endorsement by the Public Health Service or by the U.S. Department of Health and Human Services. The findings and conclusions in this report are those of the authors and do not necessarily represent the official position of the U.S. Centers for Disease Control and Prevention or any other institution.
Authors’ addresses: Jeffrey W. Priest, Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, GA, E-mail: jpriest@cdc.gov. Sukwan Handali, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, GA, E-mail: ahi0@cdc.gov.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Franco JR, Cecchi G, Priotto G, Paone M, Diarra A, Grout L, Simarro PP, Zhao W, Argaw D, 2020. Monitoring the elimination of human African trypanosomiasis at continental and country level: update to 2018. PLoS Negl Trop Dis 14: e0008261.
-
2.
World Health Organization , 2020. Ending the Neglect to Attain the Sustainable Development Goals: A Road Map for Neglected Tropical Diseases 2021–2031. Geneva, Switzerland: WHO. Available at: https://www.who.int/neglected_diseases/Revised-Draft-NTD-Roadmap-23Apr2020.pdf?ua=1. Accessed March 15, 2021.
-
3.
Jamonneau V et al., 2012. Untreated human infections by Trypanosoma brucei gambiense are not 100% fatal. PLoS Negl Trop Dis 6: e1691.
-
4.
Nzou SM, Fujii Y, Miura M, Mwau M, Mwangi AW, Itoh M, Salam MA, Hamano S, Hirayama K, Kaneko S, 2016. Development of multiplex serological assay for the detection of human African trypanosomiasis. Parasitol Int 65: 121–127.
-
5.
Arnold BF, Scobie HM, Priest JW, Lammie PJ, 2018. Integrated serologic surveillance of population immunity and disease transmission. Emerg Infect Dis 24: 1188–1194.
-
6.
Lammie PJ, Moss DM, Brook Goodhew E, Hamlin K, Krolewiecki A, West SK, Priest JW, 2012. Development of a new platform for neglected tropical disease surveillance. Int J Parasitol 42: 797–800.
-
7.
Sternberg JM, Gierlinski M, Bieler S, Ferguson MA, Ndung’u JM, 2014. Evaluation of the diagnostic accuracy of prototype rapid tests for human African trypanosomiasis. PLoS Negl Trop Dis 8: e3373.
-
8.
Sullivan L, Fleming J, Sastry L, Mehlert A, Wall SJ, Ferguson MA, 2014. Identification of sVSG117 as an immunodiagnostic antigen and evaluation of a dual-antigen lateral flow test for the diagnosis of human African trypanosomiasis. PLoS Negl Trop Dis 8: e2976.
-
9.
Sullivan L, Wall SJ, Carrington M, Ferguson MA, 2013. Proteomic selection of immunodiagnostic antigens for human African trypanosomiasis and generation of a prototype lateral flow immunodiagnostic device. PLoS Negl Trop Dis 7: e2087.
-
10.
Priest JW, Moss DM, 2020. Measuring cryptosporidium serologic responses by multiplex bead assay. Methods Mol Biol 2052: 61–85.
-
11.
Franco JR, Simarro PP, Diarra A, Ruiz-Postigo JA, Jannin JG, 2012. The human African trypanosomiasis specimen biobank: a necessary tool to support research of new diagnostics. PLoS Negl Trop Dis 6: e1571.
-
12.
Feeser KR, Cama V, Priest JW, Thiele EA, Wiegand RE, Lakwo T, Feleke SM, Cantey PT, 2017. Characterizing reactivity to Onchocerca volvulus antigens in multiplex bead assays. Am J Trop Med Hyg 97: 666–672.
-
13.
Priest JW, Stuchlik O, Reed M, Soboslay P, Cama V, Roy SL, 2020. Development of a multiplex bead assay for the detection of IgG antibody responses to guinea worm. Am J Trop Med Hyg 103: 2294–2304.
-
14.
Youden WJ, 1950. Index for rating diagnostic tests. Cancer 3: 32–35.
-
15.
Zweig MH, Campbell G, 1993. Receiver-operating characteristic (ROC) plots: a fundamental evaluation tool in clinical medicine. Clin Chem 39: 561–577.
-
16.
Youngstrom EA, 2014. A primer on receiver operating characteristic analysis and diagnostic efficiency statistics for pediatric psychology: we are ready to ROC. J Pediatr Psychol 39: 204–221.
-
17.
Simarro PP, Cecchi G, Paone M, Franco JR, Diarra A, Ruiz JA, Fevre EM, Courtin F, Mattioli RC, Jannin JG, 2010. The Atlas of human African trypanosomiasis: a contribution to global mapping of neglected tropical diseases. Int J Health Geogr 9: 57.
-
18.
Rooney B, Piening T, Buscher P, Roge S, Smales CM, 2015. Expression of Trypanosoma brucei gambiense antigens in Leishmania tarentolae. Potential for use in rapid serodiagnostic tests (RDTs). PLoS Negl Trop Dis 9: e0004271.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1669 | 1211 | 77 |
| Full Text Views | 259 | 23 | 0 |
| PDF Downloads | 130 | 27 | 0 |