In May 2021, Sasmono et al.1 published their interesting work, which reported on widescale use of antibody seroprevalence to examine the history of Zika virus (ZIKV) transmission in Indonesia. The specimens for this retrospective seroprevalence study were collected in 2014, so the stability of anti-ZIKV antibodies in the serum samples, which were stored for 6 years, should be further considered.
First, the storage conditions of the 870 serum samples, which were collected from different regions in Indonesia in 2014, were not presented in the manuscript. According to the authors, “A total of 870 serum samples from healthy 5- to 9-year-old children, which had been collected from 30 districts in 14 provinces during October and November 2014, were tested.” The storage conditions of the long-term stored sera samples should have been included in the article, and this information will be helpful in interpreting the data.
Second, there has been no report that anti-ZIKV antibodies are stable during long-term storage at –20°C or –80°C. The stability of antibody titers against ZIKV may influence the results and conclusions of seroprevalence studies. It is worth noting that the relationship between anti-ZIKV antibody titers and storage conditions should be investigated further.
This work was supported by the Foundation of Liaocheng University (318011907), the Doctoral Foundation of Liaocheng University (318051738 and 318051827), the National Natural Science Foundation of China (31802202), and the National Key Research and Development Program of China (2016YFD0500401).
Sasmono RT et al., 2021. Spatiotemporal heterogeneity of Zika virus transmission in Indonesia: serosurveillance data from a pediatric population. Am J Trop Med Hyg 104: 2220–2223.