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Evaluation of a Rapid IgG4 Lateral Flow Dipstick Test to Detect Strongyloides stercoralis Infection in Northeast Thailand

Rahmah NoordinInstitute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Penang, Malaysia;

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Nor Suhada AnuarInstitute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Penang, Malaysia;

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Nor Mustaiqazah JuriInstitute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Penang, Malaysia;

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Phattharaphon WongphutornBiomedical Science Program, Graduate School, Khon Kaen University, Khon Kaen, Thailand;
Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, Thailand

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Sirowan RuantipBiomedical Science Program, Graduate School, Khon Kaen University, Khon Kaen, Thailand;

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Kulthida Y. KopolratDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, Thailand

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Chanika WorasithDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, Thailand

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Jiraporn SithithawornFaculty of Medicine, Mahasarakham University, Mahasarakham, Thailand;

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Paiboon SithithawornDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, Thailand

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ABSTRACT.

Strongyloides stercoralis affects more than half a billion people worldwide, and hyperinfection in immunocompromised patients can be fatal. Elimination of this neglected tropical disease requires field-applicable diagnostic tools. We conducted a laboratory evaluation of a lateral flow rapid dipstick test (SsRapid™) using sera samples from a Strongyloides-endemic area in northeast Thailand. Group 1 was S. stercoralis-positive and larvae- and/or antibody-positive (according to the IgG ELISA) (N = 100). Group 2 had negative fecal examination and IgG ELISA results (N = 25). Group 3 had other parasitic infections and negative IgG ELISA results (N = 25). The results showed good diagnostic sensitivity (82%) and excellent specificity (96%). Suggested improvements in the SsRapid™ test include increased diagnostic sensitivity and conversion to the more robust cassette format. Field studies should be performed as well.

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Author Notes

Address correspondence to Rahmah Noordin, Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Penang, Malaysia. E-mail: rahmah8485@gmail.com

Authors’ addresses: Rahmah Noordin, Nor Suhada Anuar, and Nor Mustaiqazah Juri, Universiti Sains Malaysia, Institute for Research in Molecular Medicine, Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden, Penang, Malaysia 11800, E-mails: rahmah8485@gmail.com, norsuhada@usm.my, and mustaiqazah@yahoo.com. Phattharaphon Wongphutorn, Khon Kaen University, Biomedical Science Program Graduate School, Khon Kaen, Thailand and Khon Kaen University, Cholangiocarcinoma Research Institute (CARI), Nai Mueang, Khon Kaen, Thailand, E-mail: phat_phutorn@hotmail.com. Sirowan Ruantip, Khon Kaen University, Biomedical Science Program, Graduate School, Nai Mueang, Khon Kaen, Thailand, E-mail: sirowan.r@kkumail.com. Kulthida Y. Kopolrat, Chanika Worasith, and Paiboon Sithithaworn, Khon Kaen University, Parasitology, Khon Kaen, Thailand, and Khon Kaen University, Cholangiocarcinoma Research Institute (CARI), Nai Mueang, Khon Kaen, Thailand, E-mails: kulthida_kop@yahoo.com, chanika.w@kkumail.com, and paibsit@gmail.com. Jiraporn Sithithaworn, Mahasarakham University, Faculty of Medicine, Mahasarakham, Thailand, E-mail: jirapornsith@gmail.com.

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