Authors:
, , , , , , , , , , , , , , , , , and
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-
1.
Buonfrate D et al., 2020. The global prevalence of Strongyloides stercoralis infection. Pathogens 9: 468.
-
2.
Jongsuksuntigul P , Intapan PM , Wongsaroj T , Nilpan S , Singthong S , Veerakul S , Maleewong W , 2003. Prevalence of Strongyloides stercoralis infection in northeastern Thailand (agar plate culture detection). J Med Assoc Tha 86: 737–741.
-
3.
Boonroumkaew P , Sadaow L , Sanpool O , Rodpai R , Thanchomnang T , Phupiewkham W , Intapan PM , Maleewong W , 2020. Effectiveness of Strongyloides recombinant IgG immunoreactive antigen in detecting IgG and IgG4 subclass antibodies for diagnosis of human strongyloidiasis using rapid immunochromatographic tests. Diagnostics (Basel) 10: 615.
-
4.
Sadaow L , Sanpool O , Rodpai R , Boonroumkaew P , Maleewong W , Intapan PM , 2020. Development of immunochromatographic device as a point-of-care tool for serodiagnosis of human strongyloidiasis cases. Eur J Clin Microbiol Infect Dis 39: 465–470.
-
5.
Yunus MH , Arifin N , Balachandra D , Anuar NS , Noordin R , 2019. Lateral flow dipstick test for serodiagnosis of strongyloidiasis. Am J Trop Med Hyg 101: 432–435.
-
6.
Satoh M , Toma H , Sato Y , Kikuchi M , Takara M , Shiroma Y , Kiyuna S , Hirayama K , 1999. Production of a high level of specific IgG4 antibody associated with resistance to albendazole treatment in HLA-DRB1*0901-positive patients with strongyloidiasis. Am J Trop Med Hyg 61: 668–671.
-
7.
Arifin N , Hanafiah KM , Ahmad H , Noordin R , 2019. Serodiagnosis and early detection of Strongyloides stercoralis infection. J Microbiol Immunol Infect 52: 371–378.
-
8.
Norsyahida A , Riazi M , Sadjjadi SM , Muhammad Hafiznur Y , Low HC , Zeehaida M , Noordin R , 2013. Laboratory detection of strongyloidiasis: IgG-, IgG4 - and IgE-ELISAs and cross-reactivity with lymphatic filariasis. Parasite Immunol 35: 174–179.
-
9.
Sithithaworn P , Yongvanit P , Duenngai K , Kiatsopit N , Pairojkul C , 2014. Roles of liver fluke infection as risk factor for cholangiocarcinoma. J Hepatobiliary Pancreat Sci 21: 301–308.
-
10.
Koga K , Kasuya S , Khamboonruang C , Sukhavat K , Ieda M , Takatsuka N , Kita K , Ohtomo H , 1991. A modified agar plate method for detection of Strongyloides stercoralis. Am J Trop Med Hyg 45: 518–521.
-
11.
Elkins DB , Haswell-Elkins MR , Mairiang E , Mairiang P , Sithithaworn P , Kaewkes S , Bhudhisawasdi V , Uttaravichien T , 1990. A high frequency of hepatobiliary disease and suspected cholangiocarcinoma associated with heavy Opisthorchis viverrini infection in a small community in north-east Thailand. Trans R Soc Trop Med Hyg 84: 715–719.
-
12.
Sithithaworn P , Srisawangwong T , Tesana S , Daenseekaew W , Sithithaworn J , Fujimaki Y , Ando K , 2003. Epidemiology of Strongyloides stercoralis in north-east Thailand: application of the agar plate culture technique compared with the enzyme-linked immunosorbent assay. Trans R Soc Trop Med Hyg 97: 398–402.
-
13.
Eamudomkarn C , Sithithaworn P , Sithithaworn J , Kaewkes S , Sripa B , Itoh M , 2015. Comparative evaluation of Strongyloides ratti and S. stercoralis larval antigen for diagnosis of strongyloidiasis in an endemic area of opisthorchiasis. Parasitol Res 114: 2543–2551.
-
14.
Eamudomkarn C et al., 2018. Diagnostic performance of urinary IgG antibody detection: A novel approach for population screening of strongyloidiasis. PLoS One 13: e0192598.
-
15.
Makhsin SR , Razak KA , Noordin R , Zakaria ND , Chun TS , 2012. The effects of size and synthesis methods of gold nanoparticle-conjugated MαHIgG4 for use in an immunochromatographic strip test to detect brugian filariasis. Nanotechnology 23: 495719.
-
16.
Gass K , 2020. Time for a diagnostic sea-change: Rethinking neglected tropical disease diagnostics to achieve elimination. PLoS Negl Trop Dis 14: e0008933.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 3069 | 2090 | 353 |
| Full Text Views | 344 | 16 | 2 |
| PDF Downloads | 225 | 21 | 2 |
Evaluation of a Rapid IgG4 Lateral Flow Dipstick Test to Detect Strongyloides stercoralis Infection in Northeast Thailand
Rahmah Noordin
Rahmah Noordin
Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Penang, Malaysia;
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Nor Suhada Anuar
Nor Suhada Anuar
Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Penang, Malaysia;
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Nor Mustaiqazah Juri
Nor Mustaiqazah Juri
Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Penang, Malaysia;
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Phattharaphon Wongphutorn
Phattharaphon Wongphutorn
Biomedical Science Program, Graduate School, Khon Kaen University, Khon Kaen, Thailand;
Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, Thailand
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Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, Thailand
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Sirowan Ruantip
Sirowan Ruantip
Biomedical Science Program, Graduate School, Khon Kaen University, Khon Kaen, Thailand;
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Kulthida Y. Kopolrat
Kulthida Y. Kopolrat
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, Thailand
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Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, Thailand
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Chanika Worasith
Chanika Worasith
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, Thailand
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Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, Thailand
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Jiraporn Sithithaworn
Jiraporn Sithithaworn
Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand;
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Paiboon Sithithaworn
Paiboon Sithithaworn
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, Thailand
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Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, Thailand
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ABSTRACT.
Strongyloides stercoralis affects more than half a billion people worldwide, and hyperinfection in immunocompromised patients can be fatal. Elimination of this neglected tropical disease requires field-applicable diagnostic tools. We conducted a laboratory evaluation of a lateral flow rapid dipstick test (SsRapid™) using sera samples from a Strongyloides-endemic area in northeast Thailand. Group 1 was S. stercoralis-positive and larvae- and/or antibody-positive (according to the IgG ELISA) (N = 100). Group 2 had negative fecal examination and IgG ELISA results (N = 25). Group 3 had other parasitic infections and negative IgG ELISA results (N = 25). The results showed good diagnostic sensitivity (82%) and excellent specificity (96%). Suggested improvements in the SsRapid™ test include increased diagnostic sensitivity and conversion to the more robust cassette format. Field studies should be performed as well.
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Author Notes
Authors’ addresses: Rahmah Noordin, Nor Suhada Anuar, and Nor Mustaiqazah Juri, Universiti Sains Malaysia, Institute for Research in Molecular Medicine, Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden, Penang, Malaysia 11800, E-mails: rahmah8485@gmail.com, norsuhada@usm.my, and mustaiqazah@yahoo.com. Phattharaphon Wongphutorn, Khon Kaen University, Biomedical Science Program Graduate School, Khon Kaen, Thailand and Khon Kaen University, Cholangiocarcinoma Research Institute (CARI), Nai Mueang, Khon Kaen, Thailand, E-mail: phat_phutorn@hotmail.com. Sirowan Ruantip, Khon Kaen University, Biomedical Science Program, Graduate School, Nai Mueang, Khon Kaen, Thailand, E-mail: sirowan.r@kkumail.com. Kulthida Y. Kopolrat, Chanika Worasith, and Paiboon Sithithaworn, Khon Kaen University, Parasitology, Khon Kaen, Thailand, and Khon Kaen University, Cholangiocarcinoma Research Institute (CARI), Nai Mueang, Khon Kaen, Thailand, E-mails: kulthida_kop@yahoo.com, chanika.w@kkumail.com, and paibsit@gmail.com. Jiraporn Sithithaworn, Mahasarakham University, Faculty of Medicine, Mahasarakham, Thailand, E-mail: jirapornsith@gmail.com.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Buonfrate D et al., 2020. The global prevalence of Strongyloides stercoralis infection. Pathogens 9: 468.
-
2.
Jongsuksuntigul P , Intapan PM , Wongsaroj T , Nilpan S , Singthong S , Veerakul S , Maleewong W , 2003. Prevalence of Strongyloides stercoralis infection in northeastern Thailand (agar plate culture detection). J Med Assoc Tha 86: 737–741.
-
3.
Boonroumkaew P , Sadaow L , Sanpool O , Rodpai R , Thanchomnang T , Phupiewkham W , Intapan PM , Maleewong W , 2020. Effectiveness of Strongyloides recombinant IgG immunoreactive antigen in detecting IgG and IgG4 subclass antibodies for diagnosis of human strongyloidiasis using rapid immunochromatographic tests. Diagnostics (Basel) 10: 615.
-
4.
Sadaow L , Sanpool O , Rodpai R , Boonroumkaew P , Maleewong W , Intapan PM , 2020. Development of immunochromatographic device as a point-of-care tool for serodiagnosis of human strongyloidiasis cases. Eur J Clin Microbiol Infect Dis 39: 465–470.
-
5.
Yunus MH , Arifin N , Balachandra D , Anuar NS , Noordin R , 2019. Lateral flow dipstick test for serodiagnosis of strongyloidiasis. Am J Trop Med Hyg 101: 432–435.
-
6.
Satoh M , Toma H , Sato Y , Kikuchi M , Takara M , Shiroma Y , Kiyuna S , Hirayama K , 1999. Production of a high level of specific IgG4 antibody associated with resistance to albendazole treatment in HLA-DRB1*0901-positive patients with strongyloidiasis. Am J Trop Med Hyg 61: 668–671.
-
7.
Arifin N , Hanafiah KM , Ahmad H , Noordin R , 2019. Serodiagnosis and early detection of Strongyloides stercoralis infection. J Microbiol Immunol Infect 52: 371–378.
-
8.
Norsyahida A , Riazi M , Sadjjadi SM , Muhammad Hafiznur Y , Low HC , Zeehaida M , Noordin R , 2013. Laboratory detection of strongyloidiasis: IgG-, IgG4 - and IgE-ELISAs and cross-reactivity with lymphatic filariasis. Parasite Immunol 35: 174–179.
-
9.
Sithithaworn P , Yongvanit P , Duenngai K , Kiatsopit N , Pairojkul C , 2014. Roles of liver fluke infection as risk factor for cholangiocarcinoma. J Hepatobiliary Pancreat Sci 21: 301–308.
-
10.
Koga K , Kasuya S , Khamboonruang C , Sukhavat K , Ieda M , Takatsuka N , Kita K , Ohtomo H , 1991. A modified agar plate method for detection of Strongyloides stercoralis. Am J Trop Med Hyg 45: 518–521.
-
11.
Elkins DB , Haswell-Elkins MR , Mairiang E , Mairiang P , Sithithaworn P , Kaewkes S , Bhudhisawasdi V , Uttaravichien T , 1990. A high frequency of hepatobiliary disease and suspected cholangiocarcinoma associated with heavy Opisthorchis viverrini infection in a small community in north-east Thailand. Trans R Soc Trop Med Hyg 84: 715–719.
-
12.
Sithithaworn P , Srisawangwong T , Tesana S , Daenseekaew W , Sithithaworn J , Fujimaki Y , Ando K , 2003. Epidemiology of Strongyloides stercoralis in north-east Thailand: application of the agar plate culture technique compared with the enzyme-linked immunosorbent assay. Trans R Soc Trop Med Hyg 97: 398–402.
-
13.
Eamudomkarn C , Sithithaworn P , Sithithaworn J , Kaewkes S , Sripa B , Itoh M , 2015. Comparative evaluation of Strongyloides ratti and S. stercoralis larval antigen for diagnosis of strongyloidiasis in an endemic area of opisthorchiasis. Parasitol Res 114: 2543–2551.
-
14.
Eamudomkarn C et al., 2018. Diagnostic performance of urinary IgG antibody detection: A novel approach for population screening of strongyloidiasis. PLoS One 13: e0192598.
-
15.
Makhsin SR , Razak KA , Noordin R , Zakaria ND , Chun TS , 2012. The effects of size and synthesis methods of gold nanoparticle-conjugated MαHIgG4 for use in an immunochromatographic strip test to detect brugian filariasis. Nanotechnology 23: 495719.
-
16.
Gass K , 2020. Time for a diagnostic sea-change: Rethinking neglected tropical disease diagnostics to achieve elimination. PLoS Negl Trop Dis 14: e0008933.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 3069 | 2090 | 353 |
| Full Text Views | 344 | 16 | 2 |
| PDF Downloads | 225 | 21 | 2 |