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Reference Manager
BibTeX
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-
1.
Ritacco V, Di Lonardo M, Reniero A, Ambroggi M, Barrera L, Dambrosi A, Lopez B, Isola N, de Kantor IN, 1997. Nosocomial spread of human immunodeficiency virus-related multidrug-resistant tuberculosis in Buenos Aires. J Infect Dis 176: 637–642.
-
2.
Aita J, Barrera L, Reniero A, Lopez B, Biglione J, Weisburd G, Rajmil JC, Largacha C, Ritacco V, 1996. Hospital transmission of multidrug-resistant Mycobacterium tuberculosis in Rosario, Argentina. Medicina (B Aires) 56: 48–50.
-
3.
Ritacco V, López B, Ambroggi M, Palmero D, Salvadores B, Gravina E, Mazzeo E, Imaz S, Barrera L; National TB Laboratory Network, 2012. HIV infection and geographically bound transmission of drug-resistant tuberculosis, Argentina. Emerg Infect Dis 18: 1802–1810.
-
4.
Hillemann D, Warren R, Kubica T, Rüsch-Gerdes S, Niemann S, 2006. Rapid detection of Mycobacterium tuberculosis Beijing genotype strains by real-time PCR. J Clin Microbiol 44: 302–306.
-
5.
Mokrousov I, Vyazovaya A, Zhuravlev V, Otten T, Millet J, Jiao WW, Shen AD, Rastogi N, Vishnevsky B, Narvskaya O, 2014. Real-time PCR assay for rapid detection of epidemiologically and clinically significant Mycobacterium tuberculosis Beijing genotype isolates. J Clin Microbiol 52: 1691–1693.
-
6.
Millán-Lou MI et al. 2012. Rapid test for identification of a highly transmissible Mycobacterium tuberculosis Beijing strain of sub-Saharan origin. J Clin Microbiol 50: 516–518.
-
7.
Stucki D et al. 2015. Tracking a tuberculosis outbreak over 21 years: strain-specific single-nucleotide polymorphism typing combined with targeted whole-genome sequencing. J Infect Dis 211: 1306–1316.
-
8.
Eldholm V, Monteserin J, Rieux A, Lopez B, Sobkowiak B, Ritacco V, Balloux F, 2015. Four decades of transmission of a multidrug-resistant Mycobacterium tuberculosis outbreak strain. Nat Commun 6: 7119.
-
9.
Brynildsrud OB et al. 2018. Global expansion of Mycobacterium tuberculosis lineage 4 shaped by colonial migration and local adaptation. Sci Adv 4: eaat5869.
-
10.
Perez-Lago L, Martinez Lirola M, Herranz M, Comas I, Bouza E, Garcia-de-Viedma D, 2015. Fast and low-cost decentralized surveillance of transmission of tuberculosis based on strain-specific PCRs tailored from whole genome sequencing data: a pilot study. Clin Microbiol Infect 21: 249 e1–249 e9.
-
11.
Perez-Lago L, Herranz M, Comas I, Ruiz-Serrano MJ, Lopez Roa P, Bouza E, García-de-Viedma D. 2016. Ultrafast assessment of the presence of a high-risk Mycobacterium tuberculosis strain in a population. J Clin Microbiol 54: 779–781.
-
12.
Perez-Lago L, Martinez-Lirola M, Garcia S, Herranz M, Mokrousov I, Comas I, Martínez-Priego L, Bouza E, García-de-Viedma D, 2016. Urgent implementation in a hospital setting of a strategy to rule out secondary cases caused by imported extensively drug-resistant Mycobacterium tuberculosis strains at diagnosis. J Clin Microbiol 54: 2969–2974.
-
13.
Oelemann MC, Diel R, Vatin V, Haas W, Rüsch-Gerdes S, Locht C, Niemann S, Supply P, 2007. Assessment of an optimized mycobacterial interspersed repetitive unit-variable number of tandem repeat typing system combined with spoligotyping for population-based molecular epidemiology studies of tuberculosis. J Clin Microbiol 45: 691–697.
-
14.
Waisman JL, Palmero DJ, Güemes-Gurtubay JL, Videla JJ, Moretti B, Cantero M, Ambroggi MG, Poggi SE, Sancineto AE, Alberti FA, 2006. Evaluation of the control measures adopted against an epidemic of AIDS-related multidrug-resistant tuberculosis in a Latin-American Hospital (Spanish). Enferm Infecc Microbiol Clin 24: 71–76.
-
15.
Eldholm V, Rieux A, Monteserin J, Montana Lopez J, Palmero D, Lopez B, Ritacco V, Didelot X, Balloux F, 2016. Impact of human immunodeficiency virus co-infection on the evolution and transmission of multidrug-resistant tuberculosis. ELife 5: e16644.
-
16.
World Health Organization, 2018. WHO Global Tuberculosis Report 2018. Geneva, Switzerland: WHO, Licence: CC BY-NC-SA 3.0 IGO. 1.
-
17.
Hurtado RM, Meressa D, Goldfeld AE, 2018. Treatment of drug-resistant tuberculosis among people living with HIV. Curr Opin HIV AIDS 13: 478–485.
-
18.
Alonso M, Herranz M, Martinez Lirola M, Gonzalez-Rivera M, Bouza E, Garcia de Viedma D, 2012. Real-time molecular epidemiology of tuberculosis by direct genotyping of smear-positive clinical specimens. J Clin Microbiol 50: 1755–1757.
-
19.
Pérez-Lago L et al. 2017. A novel strategy based on genomics and specific PCR reveals how a multidrug-resistant Mycobacterium tuberculosis strain became prevalent in Equatorial Guinea 15 years after its emergence. Clin Microbiol Infect 23: 92–97.
-
20.
Mambuque ET, Abascal E, Venter R, Bulo H, Bouza E, Theron G, García-Basteiro AL, García-de-Viedma D, 2018. Direct genotyping of Mycobacterium tuberculosis from Xpert® MTB/RIF remnants. Tuberculosis (Edinb) 111: 202–206.
| Past two years | Past Year | Past 30 Days | |
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Trends of Two Epidemic Multidrug-Resistant Strains of Mycobacterium tuberculosis in Argentina Disclosed by Tailored Molecular Strategy
Johana Monteserin
Johana Monteserin
Instituto Nacional de Enfermedades Infecciosas ANLIS, Buenos Aires, Argentina;
Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina;
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Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina;
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Laura Pérez-Lago
Laura Pérez-Lago
Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain;
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Noemí Yokobori
Noemí Yokobori
Instituto Nacional de Enfermedades Infecciosas ANLIS, Buenos Aires, Argentina;
Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina;
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Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina;
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Roxana Paul
Roxana Paul
Instituto Nacional de Enfermedades Infecciosas ANLIS, Buenos Aires, Argentina;
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Sandra Rodríguez Maus
Sandra Rodríguez Maus
Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain;
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Norberto Simboli
Norberto Simboli
Instituto Nacional de Enfermedades Infecciosas ANLIS, Buenos Aires, Argentina;
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Vegard Eldholm
Vegard Eldholm
Norwegian Institute of Public Health, Oslo, Norway;
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Beatriz López
Beatriz López
Instituto Nacional de Enfermedades Infecciosas ANLIS, Buenos Aires, Argentina;
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Darío García de Viedma
Darío García de Viedma
Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain;
CIBER Enfermedades Respiratorias, CIBERES, Madrid, Spain
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CIBER Enfermedades Respiratorias, CIBERES, Madrid, Spain
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Viviana Ritacco
Viviana Ritacco
Instituto Nacional de Enfermedades Infecciosas ANLIS, Buenos Aires, Argentina;
Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina;
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Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina;
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Two Mycobacterium tuberculosis strains—M (sublineage 4.1) and Ra (sublineage 4.3)—have long prevailed in Argentina among patients with multidrug-resistant tuberculosis (MDR-TB). Recently, budget constraints have hampered the surveillance of MDR-TB transmission. Based on whole-genome sequence analysis, we used M- and Ra-specific single nucleotide polymorphisms to tailor two multiplex allele-specific polymerase chain reactions (PCRs), which we applied to 252 stored isolates (95% of all newly diagnosed MDR-TB cases countrywide, 2015–2017). Compared with the latest data available (2007–2009), the M strain has receded (80/324 to 20/252, P < 0.0001), particularly among cross-border migrants (12/58 to 0/53, P = 0.0003) and HIV-infected people (30/97 to 7/74, P = 0.0007), but it still accounts for 4/12 new cases of extensively drug-resistant TB. Differently, the Ra strain remained stable in frequency (39/324 to 33/252) and contributed marginally to the extensive drug-resistance load (1/12). Our novel strategy disclosed recent trends of the two major MDR-TB strains, providing meaningful data to allocate control interventions more efficiently.
Author Notes
Financial support: This work was supported by the ERANet-LAC (TRANS-TB-TRANS Project ELAC2015/T08-0664), the ISCIII (AC16/00057, 15/01554, 16/01449, 18/00599, CP15/00075, and contract number MS15/00075), and the ANPCyT (grant number PICT-2016-3219). This work was cofunded by European Regional Development Funds from the European Commission: “A way of making Europe”.
Authors’ addresses: Johana Monteserin, Noemí Yokobori, and Viviana Ritacco, Instituto Nacional de Enfermedades Infecciosas ANLIS, Buenos Aires, Argentina, and Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina, E-mails: johamonteserin@gmail.com, kaoru.noemi@gmail.com, and vivianaritacco@gmail.com. Laura Pérez-Lago and Sandra Rodríguez Maus, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain, E-mails: lperezg00@gmail.com and srodriguezm86@gmail.com. Roxana Paul, Norberto Simboli, and Beatriz López, Instituto Nacional de Enfermedades Infecciosas ANLIS, Buenos Aires, Argentina, E-mails: rpaul@anlis.gov.ar, nsimboli@anlis.gov.ar, and bealopez@anlis.gov.ar. Vegard Eldholm, Norwegian Institute of Public Health, Oslo, Norway, v.eldholm@gmail.com. Darío García de Viedma, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain, and CIBER Enfermedades Respiratorias, CIBERES, Madrid, Spain, E-mail: dgviedma2@gmail.com.
These authors contributed equally to this work.
These authors contributed equally to this work.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Ritacco V, Di Lonardo M, Reniero A, Ambroggi M, Barrera L, Dambrosi A, Lopez B, Isola N, de Kantor IN, 1997. Nosocomial spread of human immunodeficiency virus-related multidrug-resistant tuberculosis in Buenos Aires. J Infect Dis 176: 637–642.
-
2.
Aita J, Barrera L, Reniero A, Lopez B, Biglione J, Weisburd G, Rajmil JC, Largacha C, Ritacco V, 1996. Hospital transmission of multidrug-resistant Mycobacterium tuberculosis in Rosario, Argentina. Medicina (B Aires) 56: 48–50.
-
3.
Ritacco V, López B, Ambroggi M, Palmero D, Salvadores B, Gravina E, Mazzeo E, Imaz S, Barrera L; National TB Laboratory Network, 2012. HIV infection and geographically bound transmission of drug-resistant tuberculosis, Argentina. Emerg Infect Dis 18: 1802–1810.
-
4.
Hillemann D, Warren R, Kubica T, Rüsch-Gerdes S, Niemann S, 2006. Rapid detection of Mycobacterium tuberculosis Beijing genotype strains by real-time PCR. J Clin Microbiol 44: 302–306.
-
5.
Mokrousov I, Vyazovaya A, Zhuravlev V, Otten T, Millet J, Jiao WW, Shen AD, Rastogi N, Vishnevsky B, Narvskaya O, 2014. Real-time PCR assay for rapid detection of epidemiologically and clinically significant Mycobacterium tuberculosis Beijing genotype isolates. J Clin Microbiol 52: 1691–1693.
-
6.
Millán-Lou MI et al. 2012. Rapid test for identification of a highly transmissible Mycobacterium tuberculosis Beijing strain of sub-Saharan origin. J Clin Microbiol 50: 516–518.
-
7.
Stucki D et al. 2015. Tracking a tuberculosis outbreak over 21 years: strain-specific single-nucleotide polymorphism typing combined with targeted whole-genome sequencing. J Infect Dis 211: 1306–1316.
-
8.
Eldholm V, Monteserin J, Rieux A, Lopez B, Sobkowiak B, Ritacco V, Balloux F, 2015. Four decades of transmission of a multidrug-resistant Mycobacterium tuberculosis outbreak strain. Nat Commun 6: 7119.
-
9.
Brynildsrud OB et al. 2018. Global expansion of Mycobacterium tuberculosis lineage 4 shaped by colonial migration and local adaptation. Sci Adv 4: eaat5869.
-
10.
Perez-Lago L, Martinez Lirola M, Herranz M, Comas I, Bouza E, Garcia-de-Viedma D, 2015. Fast and low-cost decentralized surveillance of transmission of tuberculosis based on strain-specific PCRs tailored from whole genome sequencing data: a pilot study. Clin Microbiol Infect 21: 249 e1–249 e9.
-
11.
Perez-Lago L, Herranz M, Comas I, Ruiz-Serrano MJ, Lopez Roa P, Bouza E, García-de-Viedma D. 2016. Ultrafast assessment of the presence of a high-risk Mycobacterium tuberculosis strain in a population. J Clin Microbiol 54: 779–781.
-
12.
Perez-Lago L, Martinez-Lirola M, Garcia S, Herranz M, Mokrousov I, Comas I, Martínez-Priego L, Bouza E, García-de-Viedma D, 2016. Urgent implementation in a hospital setting of a strategy to rule out secondary cases caused by imported extensively drug-resistant Mycobacterium tuberculosis strains at diagnosis. J Clin Microbiol 54: 2969–2974.
-
13.
Oelemann MC, Diel R, Vatin V, Haas W, Rüsch-Gerdes S, Locht C, Niemann S, Supply P, 2007. Assessment of an optimized mycobacterial interspersed repetitive unit-variable number of tandem repeat typing system combined with spoligotyping for population-based molecular epidemiology studies of tuberculosis. J Clin Microbiol 45: 691–697.
-
14.
Waisman JL, Palmero DJ, Güemes-Gurtubay JL, Videla JJ, Moretti B, Cantero M, Ambroggi MG, Poggi SE, Sancineto AE, Alberti FA, 2006. Evaluation of the control measures adopted against an epidemic of AIDS-related multidrug-resistant tuberculosis in a Latin-American Hospital (Spanish). Enferm Infecc Microbiol Clin 24: 71–76.
-
15.
Eldholm V, Rieux A, Monteserin J, Montana Lopez J, Palmero D, Lopez B, Ritacco V, Didelot X, Balloux F, 2016. Impact of human immunodeficiency virus co-infection on the evolution and transmission of multidrug-resistant tuberculosis. ELife 5: e16644.
-
16.
World Health Organization, 2018. WHO Global Tuberculosis Report 2018. Geneva, Switzerland: WHO, Licence: CC BY-NC-SA 3.0 IGO. 1.
-
17.
Hurtado RM, Meressa D, Goldfeld AE, 2018. Treatment of drug-resistant tuberculosis among people living with HIV. Curr Opin HIV AIDS 13: 478–485.
-
18.
Alonso M, Herranz M, Martinez Lirola M, Gonzalez-Rivera M, Bouza E, Garcia de Viedma D, 2012. Real-time molecular epidemiology of tuberculosis by direct genotyping of smear-positive clinical specimens. J Clin Microbiol 50: 1755–1757.
-
19.
Pérez-Lago L et al. 2017. A novel strategy based on genomics and specific PCR reveals how a multidrug-resistant Mycobacterium tuberculosis strain became prevalent in Equatorial Guinea 15 years after its emergence. Clin Microbiol Infect 23: 92–97.
-
20.
Mambuque ET, Abascal E, Venter R, Bulo H, Bouza E, Theron G, García-Basteiro AL, García-de-Viedma D, 2018. Direct genotyping of Mycobacterium tuberculosis from Xpert® MTB/RIF remnants. Tuberculosis (Edinb) 111: 202–206.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 386 | 279 | 20 |
| Full Text Views | 607 | 7 | 0 |
| PDF Downloads | 235 | 8 | 0 |