Author:
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-
1.
Gloria-soria A et al. 2016. Global genetic diversity of Aedes aegypti. Mol Ecol 25: 5377–5395.
-
2.
Kraemer MU et al. 2015. The global distribution of the arbovirus vectors Aedes aegypti and Ae. albopictus. Elife 4: 1–18.
-
3.
Lin CH, Schiøler KL, Jepsen MR, Ho CK, Li SH, Konradsen F, 2012. Dengue outbreaks in high-income area, Kaohsiung city, Taiwan, 2003–2009. Emerg Infect Dis 18: 1603–1611.
-
4.
Lin CH, Schiøler KL, Ekstrøm CT, Konradsen F, 2018. Location, seasonal and functional characteristics of water holding containers with juvenile and pupal Aedes aegypti in southern Taiwan: a cross-sectional study using hurdle model analyses. PLoS Negl Trop Dis 12: e0006882.
-
5.
Smith LB, Kasai S, Scott JG, 2016. Pyrethroid resistance in Aedes aegypti and Aedes albopictus: important mosquito vectors of human diseases. Pestic Biochem Physiol 133: 1–12.
-
6.
Amelia-Yap ZH, Chen CD, Sofian-Azirun M, Low VL, 2018. Pyrethroid resistance in the dengue vector Aedes aegypti in southeast Asia: present situation and prospects for management. Parasit Vectors 11: 332.
-
7.
Hsia W, Wu H, Yang Y, Lin C, 2011. Efficacy of commonly used insecticides to Aedes aegypti in southern Taiwan. Taiwan Epi Bul 27: 39–49.
-
8.
Lin Y-H, Tsen WL, Tien NY, Luo YP, 2013. Biochemical and molecular analyses to determine pyrethroid resistance in Aedes aegypti. Pestic Biochem Physiol. 107: 266–276.
-
9.
Moyes CL et al. 2017. Contemporary status of insecticide resistance in the major Aedes vectors of arboviruses infecting humans. PLoS Negl Trop Dis 11: 1–20.
-
10.
Plernsub S, Saingamsook J, Yanola J, Lumjuan N, Tippawangkosol P, Sukontason K, Walton C, Somboon P, 2016. Additive effect of knockdown resistance mutations, S989P, V1016G and F1534C, in a heterozygous genotype conferring pyrethroid resistance in Aedes aegypti in Thailand. Parasit Vectors 9: 417.
-
11.
Chang C, Shen WK, Wang TT, Lin YH, Hsu EL, Dai SM, 2009. A novel amino acid substitution in a voltage-gated sodium channel is associated with knockdown resistance to permethrin in Aedes aegypti. Insect Biochem Mol Biol 39: 272–278.
-
12.
Collins FH, Mendez MA, Rasmussen MO, Mehaffey PC, Besansky NJ, Finnerty V, 1987. A ribosomal RNA gene probe differentiates member species of the Anopheles gambiae complex. Am J Trop Med Hyg 37: 37–41.
-
13.
Higa Y, Toma T, Tsuda Y, Miyagi I, 2010. A multiplex PCR-based molecular identification of five morphologically related, medically important subgenus stegomyia mosquitoes from the genus Aedes (Diptera: Culicidae) found in the Ryukyu archipelago, Japan. Jpn J Infect Dis 63: 312–316.
-
14.
Li CX et al. 2015. Relationship between insecticide resistance and kdr mutations in the dengue vector Aedes aegypti in southern China. Parasit Vectors 8: 325.
-
15.
Yanola J, Somboon P, Walton C, Nachaiwieng W, Somwang P, Prapanthadara L, 2011. High-throughput assays for detection of the F1534C mutation in the voltage-gated sodium channel gene in permethrin-resistant Aedes aegypti and the distribution of this mutation throughout Thailand. Trop Med Int Heal 16: 501–509.
-
16.
Faucon F et al. 2015. Identifying genomic changes associated with insecticide resistance in the dengue mosquito Aedes aegypti by deep targeted sequencing. Genome Res 1347–1359.
-
17.
Chung HH, Cheng IC, Chen YC, Lin C, Tomita T, Teng HJ, 2019. Voltage-gated sodium channel intron polymorphism and four mutations comprise six haplotypes in an Aedes aegypti population in Taiwan. PLoS Negl Trop Dis 13: e0007291.
-
18.
Ishak IH, Jaal Z, Ranson H, Wondji CS, 2015. Contrasting patterns of insecticide resistance and knockdown resistance (kdr) in the dengue vectors Aedes aegypti and Aedes albopictus from Malaysia. Parasit Vectors 8: 181.
-
19.
Vera-Maloof FZ, Saavedra-Rodriguez K, Elizondo-Quiroga AE, Lozano-Fuentes S, Black WC IV, 2015. Coevolution of the Ile1,016 and Cys1,534 mutations in the voltage gated sodium channel gene of Aedes aegypti in Mexico. PLoS Negl Trop Dis 9: e0004263.
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| Abstract Views | 34 | 34 | 14 |
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Temporal Pattern of Mutations in the Knockdown Resistance (kdr) Gene of Aedes aegypti Mosquitoes Sampled from Southern Taiwan
Sandrine Biduda
Sandrine Biduda
Department of Immunology and Microbiology, Centre for Medical Parasitology, University of Copenhagen, Copenhagen, Denmark;
Department of Infectious Diseases, Copenhagen University Hospital, Copenhagen, Denmark;
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Department of Infectious Diseases, Copenhagen University Hospital, Copenhagen, Denmark;
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Chia-Hsien Lin
Chia-Hsien Lin
Department of Public Health, Global Health Section, University of Copenhagen, Copenhagen, Denmark;
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Fatma Saleh
Fatma Saleh
Department of Allied Health Sciences, School of Health and Medical Sciences, The State University of Zanzibar, Zanzibar, Tanzania
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Flemming Konradsen
Flemming Konradsen
Department of Public Health, Global Health Section, University of Copenhagen, Copenhagen, Denmark;
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Helle Hansson
Helle Hansson
Department of Immunology and Microbiology, Centre for Medical Parasitology, University of Copenhagen, Copenhagen, Denmark;
Department of Infectious Diseases, Copenhagen University Hospital, Copenhagen, Denmark;
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Department of Infectious Diseases, Copenhagen University Hospital, Copenhagen, Denmark;
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Karin L. Schiøler
Karin L. Schiøler
Department of Public Health, Global Health Section, University of Copenhagen, Copenhagen, Denmark;
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Michael Alifrangis
Michael Alifrangis
Department of Immunology and Microbiology, Centre for Medical Parasitology, University of Copenhagen, Copenhagen, Denmark;
Department of Infectious Diseases, Copenhagen University Hospital, Copenhagen, Denmark;
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Department of Infectious Diseases, Copenhagen University Hospital, Copenhagen, Denmark;
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Aedes mosquitoes are the principal dengue vector in Taiwan, where the use of insecticides is a key element in the national control strategy. However, control efforts are constrained by the development of resistance to most insecticides, including pyrethroids. In this study, mutations in the voltage-gated sodium channel (VGSC) gene resulting in knockdown resistance (kdr) were examined in Aedes aegypti. Fragments of the VGSC gene were polymerase chain reaction (PCR)-amplified followed by restriction fragment length polymorphism analysis in samples from various settings in Southern Taiwan covering dry and wet seasons from 2013 to 2015. Three kdr mutations were identified: V1023G, D1794Y, and F1534C, with observed frequencies of 0.36, 0.55, and 0.33, respectively, in the dry season of 2013–2014. Exploring for temporal changes, the most important observation was the 1534C allele frequency increment in the following season to 0.60 (P < 0.05). This study suggests that continued insecticide pressure is driving the mutational changes, although the selection is ambiguous in the mosquito population.
Author Notes
Financial support: This study was supported by the University of Copenhagen.
Authors’ addresses: Sandrine Biduda, Helle Hansson, and Michael Alifrangis, Center for Medical Parasitology, University of Copenhagen, Copenhagen, Denmark, E-mails: sandrine@sund.ku.dk, hellehan@sund.ku.dk, and micali@sund.ku.dk. Chia-Hsien Lin, Karin Linda Schiøler, and Flemming Konradsen, Department of Public Health, School of Global Health, University of Copenhagen, Copenhagen, Denmark, E-mails: chlin@sund.ku.dk, ksch@sund.ku.dk, and flko@sund.ku.dk. Fatma Saleh, Department of Allied Health Sciences, School of Health and Medical Sciences, The State University of Zanzibar, Zanzibar, Tanzania, E-mail: fatmahamidsaleh@gmail.com.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Gloria-soria A et al. 2016. Global genetic diversity of Aedes aegypti. Mol Ecol 25: 5377–5395.
-
2.
Kraemer MU et al. 2015. The global distribution of the arbovirus vectors Aedes aegypti and Ae. albopictus. Elife 4: 1–18.
-
3.
Lin CH, Schiøler KL, Jepsen MR, Ho CK, Li SH, Konradsen F, 2012. Dengue outbreaks in high-income area, Kaohsiung city, Taiwan, 2003–2009. Emerg Infect Dis 18: 1603–1611.
-
4.
Lin CH, Schiøler KL, Ekstrøm CT, Konradsen F, 2018. Location, seasonal and functional characteristics of water holding containers with juvenile and pupal Aedes aegypti in southern Taiwan: a cross-sectional study using hurdle model analyses. PLoS Negl Trop Dis 12: e0006882.
-
5.
Smith LB, Kasai S, Scott JG, 2016. Pyrethroid resistance in Aedes aegypti and Aedes albopictus: important mosquito vectors of human diseases. Pestic Biochem Physiol 133: 1–12.
-
6.
Amelia-Yap ZH, Chen CD, Sofian-Azirun M, Low VL, 2018. Pyrethroid resistance in the dengue vector Aedes aegypti in southeast Asia: present situation and prospects for management. Parasit Vectors 11: 332.
-
7.
Hsia W, Wu H, Yang Y, Lin C, 2011. Efficacy of commonly used insecticides to Aedes aegypti in southern Taiwan. Taiwan Epi Bul 27: 39–49.
-
8.
Lin Y-H, Tsen WL, Tien NY, Luo YP, 2013. Biochemical and molecular analyses to determine pyrethroid resistance in Aedes aegypti. Pestic Biochem Physiol. 107: 266–276.
-
9.
Moyes CL et al. 2017. Contemporary status of insecticide resistance in the major Aedes vectors of arboviruses infecting humans. PLoS Negl Trop Dis 11: 1–20.
-
10.
Plernsub S, Saingamsook J, Yanola J, Lumjuan N, Tippawangkosol P, Sukontason K, Walton C, Somboon P, 2016. Additive effect of knockdown resistance mutations, S989P, V1016G and F1534C, in a heterozygous genotype conferring pyrethroid resistance in Aedes aegypti in Thailand. Parasit Vectors 9: 417.
-
11.
Chang C, Shen WK, Wang TT, Lin YH, Hsu EL, Dai SM, 2009. A novel amino acid substitution in a voltage-gated sodium channel is associated with knockdown resistance to permethrin in Aedes aegypti. Insect Biochem Mol Biol 39: 272–278.
-
12.
Collins FH, Mendez MA, Rasmussen MO, Mehaffey PC, Besansky NJ, Finnerty V, 1987. A ribosomal RNA gene probe differentiates member species of the Anopheles gambiae complex. Am J Trop Med Hyg 37: 37–41.
-
13.
Higa Y, Toma T, Tsuda Y, Miyagi I, 2010. A multiplex PCR-based molecular identification of five morphologically related, medically important subgenus stegomyia mosquitoes from the genus Aedes (Diptera: Culicidae) found in the Ryukyu archipelago, Japan. Jpn J Infect Dis 63: 312–316.
-
14.
Li CX et al. 2015. Relationship between insecticide resistance and kdr mutations in the dengue vector Aedes aegypti in southern China. Parasit Vectors 8: 325.
-
15.
Yanola J, Somboon P, Walton C, Nachaiwieng W, Somwang P, Prapanthadara L, 2011. High-throughput assays for detection of the F1534C mutation in the voltage-gated sodium channel gene in permethrin-resistant Aedes aegypti and the distribution of this mutation throughout Thailand. Trop Med Int Heal 16: 501–509.
-
16.
Faucon F et al. 2015. Identifying genomic changes associated with insecticide resistance in the dengue mosquito Aedes aegypti by deep targeted sequencing. Genome Res 1347–1359.
-
17.
Chung HH, Cheng IC, Chen YC, Lin C, Tomita T, Teng HJ, 2019. Voltage-gated sodium channel intron polymorphism and four mutations comprise six haplotypes in an Aedes aegypti population in Taiwan. PLoS Negl Trop Dis 13: e0007291.
-
18.
Ishak IH, Jaal Z, Ranson H, Wondji CS, 2015. Contrasting patterns of insecticide resistance and knockdown resistance (kdr) in the dengue vectors Aedes aegypti and Aedes albopictus from Malaysia. Parasit Vectors 8: 181.
-
19.
Vera-Maloof FZ, Saavedra-Rodriguez K, Elizondo-Quiroga AE, Lozano-Fuentes S, Black WC IV, 2015. Coevolution of the Ile1,016 and Cys1,534 mutations in the voltage gated sodium channel gene of Aedes aegypti in Mexico. PLoS Negl Trop Dis 9: e0004263.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 34 | 34 | 14 |
| Full Text Views | 555 | 113 | 1 |
| PDF Downloads | 231 | 38 | 0 |