Cytotaxonomy of Trypanosoma cruzi (Chagas, 1909): Differentiation of T. cruzi I (TcI) and T. cruzi II (TcII) Genotypes Using Cytogenetic Markers

Ana Beatriz Bortolozo de Oliveira Laboratório de Biologia Celular, Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista “Júlio de Mesquita Filho”, IBILCE/UNESP, São José do Rio Preto, Brazil;

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Aline Rimoldi Ribeiro Departamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, UNICAMP-Avenida Bertrand Russel, Campinas, Brazil;

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Fernanda Fernandez Madeira Laboratório de Biologia Celular, Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista “Júlio de Mesquita Filho”, IBILCE/UNESP, São José do Rio Preto, Brazil;

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Natália Regina Cesaretto Instituto de Biociências de Botucatu, Universidade Estadual Paulista “Júlio de Mesquita Filho”, IBB/UNESP, Botucatu, Brazil;

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João Aristeu da Rosa Laboratório de Parasitologia, Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista “Júlio de Mesquita Filho”, FCFAR/UNESP, Araraquara, São Paulo, Brazil

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Maria Tercília Vilela de Azeredo-Oliveira Laboratório de Biologia Celular, Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista “Júlio de Mesquita Filho”, IBILCE/UNESP, São José do Rio Preto, Brazil;

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Kaio Cesar Chaboli Alevi Instituto de Biociências de Botucatu, Universidade Estadual Paulista “Júlio de Mesquita Filho”, IBB/UNESP, Botucatu, Brazil;
Laboratório de Parasitologia, Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista “Júlio de Mesquita Filho”, FCFAR/UNESP, Araraquara, São Paulo, Brazil

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Chagas disease is a public health problem caused by the Trypanosoma cruzi, and the T. cruzi I (TcI) and T. cruzi II (TcII) groups are considered important genotypes from the clinical point of view. Currently, the groups need to be molecularly analyzed for their identification; thus, we cytogenetically analyzed these groups with the objective of developing more accessible techniques for the characterization of these parasites. TcI and TcII groups were differentiated by nucleus characterization with lacto-acetic orcein (TcI—nucleus with positive heteropycnosis and TcII—nucleus with negative heteropycnosis), emphasizing the importance of the application of this technique for epidemiological and clinical studies of Chagas disease.

Author Notes

Address correspondence to Ana Beatriz Bortolozo de Oliveira, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista “Júlio de Mesquita Filho”, IBILCE/UNESP, Rua Cristóvão Colombo 2265, São José do Rio Preto, Brazil. E-mail: anabbortolozo@gmail.com

Authors’ addresses: Ana Beatriz Bortolozo de Oliveira, Fernanda Fernandez Madeira, and Maria Tercília Vilela de Azeredo-Oliveira, Laboratório de Biologia Celular, Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista “Júlio de Mesquita Filho”, IBILCE/UNESP, São José do Rio Preto, Brazil, E-mails: anabbortolozo@gmail.com, fernanda.bio56@hotmail.com, and tercilia@ibilce.unesp.br. Aline Rimoldi Ribeiro, Departamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, UNICAMP-Avenida Bertrand Russel, Campinas, Brazil, E-mail: line2rimoldi@gmail.com. Natália Regina Cesaretto, Instituto de Biociências de Botucatu, Universidade Estadual Paulista “Júlio de Mesquita Filho”, IBB/UNESP, Botucatu, Brazil, E-mail: nrcesaretto@gmail.com. João Aristeu da Rosa and Kaio Cesar Chaboli Alevi, Laboratório de Parasitologia, Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista “Júlio de Mesquita Filho”, FCFAR/UNESP, Araraquara, Brazil, E-mails: joaoaristeu@gmail.com and kaiochaboli@hotmail.com.

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