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Lateral Flow Dipstick Test for Serodiagnosis of Strongyloidiasis

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  • 1 Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Pulau Pinang, Malaysia
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The conventional method of detecting Strongyloides stercoralis in fecal samples has poor diagnostic sensitivity. Detection of Strongyloides-specific antibodies increases the sensitivity; however, most tests are ELISAs that use parasite extract which may cross-react with the sera of other helminth infections. To improve the serological diagnosis of strongyloidiasis, this study aimed at developing a sensitive and specific lateral flow rapid dipstick test. Two recombinant proteins, recombinant NIE (rNIE) and recombinant Ss1a (rSs1a), were used in preparing the dipstick, with gold-conjugated antihuman IgG4 as detector reagent. In parallel, the corresponding ELISA was performed. Both assays demonstrated diagnostic sensitivity of 91.3% (21/23) when tested with serum samples of patients with Strongyloides infection, and 100% specificity with 82 sera of asymptomatic (healthy) and those with other parasitic infections. The ELISA and dipstick test results were positively correlated to each other (r = 0.6114, P = 0.0019). The developed lateral flow dipstick test may improve the serodiagnosis of strongyloidiasis and merit further validation studies.

Author Notes

Address correspondence to Rahmah Noordin, Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia. E-mail: rahmah8485@gmail.com

Disclosure: R. N. and N. A. are named as inventors in a patent application titled “Strongyloides stercoralis protein and/or corresponding DNA and RNA sequences for application in diagnosis” filed in Malaysia (PI 2015002836) and the United States, No. 15/778430.

Financial support: This study was funded by Universiti Sains Malaysia Bridging Grant [304/CIPPM/6316064] and Ministry of Education through the Higher Institution Centre of Excellence (HICoE) program [Grant no. 311/CIPPM/4401005].

Authors’ addresses: Muhammad Hafiznur Yunus, Norsyahida Arifin, Dinesh Balachandra, Nor Suhada Anuar, and Rahmah Noordin, Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Pulau Pinang, Malaysia, E-mails: mhn1016@yahoo.com, syahidaarifin@gmail.com, dineshpenangfree@gmail.com, norsuhada@usm.my, and rahmah8485@gmail.com.

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