Detection of Taenia solium DNA in the Urine of Neurocysticercosis Patients

Luz Toribio Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru;

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Miryam Romano W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland;

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Alan L. Scott W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland;

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Isidro Gonzales Cysticercosis Unit, Instituto Nacional de Ciencias Neurológicas, Lima, Peru

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Herbert Saavedra Cysticercosis Unit, Instituto Nacional de Ciencias Neurológicas, Lima, Peru

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Hector H. Garcia Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru;
Cysticercosis Unit, Instituto Nacional de Ciencias Neurológicas, Lima, Peru

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Clive Shiff W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland;

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for the Cysticercosis Working Group in Peru Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru;
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland;
Cysticercosis Unit, Instituto Nacional de Ciencias Neurológicas, Lima, Peru

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DOI:
https://doi.org/10.4269/ajtmh.18-0706
Volume/Issue:
Volume 100: Issue 2
Page(s):
327–329
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Neurocysticercosis (NCC), caused by Taenia solium larvae that reside in the central nervous system, results in serious public health and medical issues in many regions of the world. Current diagnosis of NCC is complex requiring both serology and costly neuroimaging of parasitic cysts in the brain. This diagnostic pipeline can be problematic in resource-constrained settings. There is an unmet need for a highly sensitive and clinically informative diagnostic test to complement the present diagnostic approaches. Here, we report that T. solium–derived cell-free DNA is readily detectable in the urine of patients with the subarachnoid and parenchymal forms of NCC, and discuss the potential utility of this approach in enhancing and refining T. solium diagnostics.

Author Notes

Address correspondence to Clive Shiff, W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD. E-mail: cshiff1@jhu.edu

Financial support: This study was partially supported by grants U19AI129909 from the National Institute of Allergy and Infectious Diseases (Peru TMRC Program, NIAID/NIH), R01NS054805 from the National Institute of Neurological Disorders and Stroke (NINDS/NIH), and D43TW001140 training grant from the Fogarty International Center (FIC/NIH).

Authors’ addresses: Luz Toribio, Centro de Salud Global Tumbes, Universidad Peruana Cayetano Heredia, Lima, Peru, E-mail: luztoribio14s@gmail.com. Miryam Romano and Alan L. Scott, Department of Immunology and Infectious Diseases, Johns Hopkins School of Public Health, Baltimore, MD, E-mails: miryamdelosangelesromano@gmail.com and ascott@jhsph.edu. Isidro Gonzales and Herbert Saavedra, Cystercosis Unit, Instituto Nacional de Ciecias Neurologicas, Lima, Peru, E-mails: igonzales@gmail.com and hsaavedra@gmail.com. Herbert H. Garcia, Centro de Salud Global Tumbes, Universidad Peruana Cayetano Heredia, Lima, Peru, and Department of Microbiology, School of Sciences, Universidad Peruana Cayetano Heredia, Lima, Peru, E-mail: hgarcia1@jhu.edu. Clive Shiff, Department of Molecular Microbiology and Immunology, Johns Hopkins University, Baltimore, MD, E-mail: cshiff1@jhu.edu.

Cysticercosis Working Group in Peru: Hector H. Garcia, MD, PhD; Robert H. Gilman, MD, DTMH; Armando E. Gonzalez, DVM, PhD; and Victor C. W. Tsang, PhD (Coordination Board); Silvia Rodriguez, MSc; Isidro Gonzalez, MD; Herbert Saavedra, MD; Manuel Martinez, MD (Instituto Nacional de Ciencias Neurológicas, Lima, Perú); Manuela Verastegui, PhD; Mirko Zimic, PhD; Javier Bustos, MD, MPH; Saul Santivanez, MD, PhD; Holger Mayta, PhD; Yesenia Castillo, MSc; Monica Pajuelo, PhD; Gianfranco Arroyo, DVM, MSc (Universidad Peruana Cayetano Heredia, Lima, Perú); Maria T. Lopez, DVM, PhD; Luis Gomez, DVM; Cesar M. Gavidia, DVM, PhD (School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Perú); Luz M. Moyano, MD; Ricardo Gamboa, MSc; Claudio Muro; Percy Vichez, MSc (Cysticercosis Elimination Program, Tumbes, Perú); Sukwan Handali, MD; John Noh (Centers for Disease Control, Atlanta, GA); Theodore E. Nash, MD; Siddhartha Mahanty, MD, PhD (NIAID, NIH, Bethesda, MD); Jon Friedland (Imperial College, London, United Kingdom).

Copyright:
© The American Society of Tropical Medicine and Hygiene 2019
Received:
27 Aug 2018
|
Accepted:
22 Oct 2018
|
Published Online:
10 Dec 2018
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Online ISSN:
1476-1645
  • 1.

    Garcia HH, Nash TE, Del Brutto OH, 2014. Clinical symptoms, diagnosis and treatment of neurocysticercosis. Lancet Neurol 13: 1202–1215.

  • 2.

    Garcia HH, Parkhouse RM, Gilman RH, Montenegro T, Bernal T, Martinez SM, Gonzalez AE, Tsang VC, Harrison LJ; Cysticercosis Working Group in Peru, 2000. Serum antigen detection in the diagnosis, treatment, and follow-up of neurocysticercosis patients. Trans R Soc Trop Med Hyg 94: 673–676.

    • Search Google Scholar
    • Export Citation
  • 3.

    Erhart A et al. 2002. Taenia solium cysticercosis in a village in northern Viet Nam: seroprevalence study using an ELISA for detecting circulating antigen. Trans R Soc Trop Med Hyg 96: 270–272.

    • Search Google Scholar
    • Export Citation
  • 4.

    Tsang VC, Brand JA, Boyer AE, 1989. An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). J Infect Dis 159: 50–59.

    • Search Google Scholar
    • Export Citation
  • 5.

    Wagner J, 2012. Free DNA—new potential analyte in clinical laboratory diagnostics? Biochemia Med 22: 24–38.

  • 6.

    Weerakoon KG, McManus DP, 2016. Cell-free DNA as a diagnostic tool for human parasitic infections. Trends Parasitol 32: 378–391.

  • 7.

    Yu J, Gu G, Ju S, 2014. Recent advances in clinical applications of circulating cell-free DNA integrity. Lab Med 45: 6–11.

  • 8.

    Chapman A, Vallejo V, Mossie KG, Ortiz D, Agabian N, Flisser A, 1995. Isolation and characterization of species-specific DNA probes from Taenia solium and Taenia saginata and their use in an egg detection assay. J Clin Microbiol 33: 1283–1288.

    • Search Google Scholar
    • Export Citation
  • 9.

    Ibironke O, Koukounari A, Asaolu S, Moustaki I, Shiff C, 2012. Validation of a new test for Schistosoma haematobium based on detection of Dra1 DNA fragments in urine: evaluation through latent class analysis. PLoS Negl Trop Dis 6: e1464.

    • Search Google Scholar
    • Export Citation
  • 10.

    Krolewiecki AJ, Koukounari A, Romano M, Caro RN, Scott AL, Fleitas P, Cimino R, Shiff CJ, 2018. Transrenal DNA-based diagnosis of Strongyloides stercoralis (Grassi, 1879) infection: Bayesian latent class modeling of test accuracy. PLoS Negl Trop Dis 12: e0006550.

    • Search Google Scholar
    • Export Citation
  • 11.

    Lodh N, Caro R, Sofer S, Scott A, Krolewiecki A, Shiff C, 2016. Diagnosis of Strongyloides stercoralis: detection of parasite-derived DNA in urine. Acta Trop 163: 9–13.

    • Search Google Scholar
    • Export Citation
  • 12.

    Lodh N, Mwansa JC, Mutengo MM, Shiff CJ, 2013. Diagnosis of Schistosoma mansoni without the stool: comparison of three diagnostic tests to detect Schistosoma [corrected] mansoni infection from filtered urine in Zambia. Am J Trop Med Hyg 89: 46–50.

    • Search Google Scholar
    • Export Citation
  • 13.

    Almeida CR, Ojopi EP, Nunes CM, Machado LR, Takayanagui OM, Livramento JA, Abraham R, Gattaz WF, Vaz AJ, Dias-Neto E, 2006. Taenia solium DNA is present in the cererbospinal fluid of neurocysticercosis patients and can be used for diagnosis. Eur Arch Psychiatry Clin Neurosci 256: 307–310.

    • Search Google Scholar
    • Export Citation
  • 14.

    Khier S, Lohan L, 2018. Kinetics of circulating cell-free DNA for biomedical applications: critical appraisal of the literature. Future Sci OA 4: FSO295.

    • Search Google Scholar
    • Export Citation
  • 15.

    An M, Gao Y, 2015. Urinary biomarkers of brain diseases. Genomics, Proteomics Bioinformatics 13: 345–354.

  • 16.

    Dawson J, Walters M, Delles C, Mischak H, Mullen W, 2012. Urinary proteomics to support diagnosis of stroke. PLoS One 7: e35879.

  • 17.

    Prasad S, Tyagi AK, Aggarwal BB, 2016. Detection of inflammatory biomarkers in saliva and urine: potential in diagnosis, prevention, and treatment for chronic diseases. Exp Biol Med (Maywood) 241: 783–799.

    • Search Google Scholar
    • Export Citation
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