Case Report: Cervicovaginal Co-Colonization with Entamoeba gingivalis and Entamoeba polecki in Association with an Intrauterine Device

Richard S. Bradbury Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia;

Search for other papers by Richard S. Bradbury in
Current site
Google Scholar
PubMed Close
,
Shantanu Roy Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia;

Search for other papers by Shantanu Roy in
Current site
Google Scholar
PubMed Close
,
Ibne K. Ali Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia;

Search for other papers by Ibne K. Ali in
Current site
Google Scholar
PubMed Close
,
Jasmin R. Morrison UnityPoint Medical Primary Care, Johnston, Iowa;

Search for other papers by Jasmin R. Morrison in
Current site
Google Scholar
PubMed Close
,
Dale Waldner UnityPoint Health, Cedar Rapids, Iowa;

Search for other papers by Dale Waldner in
Current site
Google Scholar
PubMed Close
,
Katherine Hebbeln State Hygienic Laboratory at the University of Iowa, Coralville, Iowa

Search for other papers by Katherine Hebbeln in
Current site
Google Scholar
PubMed Close
,
Wade Aldous State Hygienic Laboratory at the University of Iowa, Coralville, Iowa

Search for other papers by Wade Aldous in
Current site
Google Scholar
PubMed Close
,
Ryan Jepson State Hygienic Laboratory at the University of Iowa, Coralville, Iowa

Search for other papers by Ryan Jepson in
Current site
Google Scholar
PubMed Close
,
Haleigh R. Delavan UnityPoint Medical Primary Care, Johnston, Iowa;

Search for other papers by Haleigh R. Delavan in
Current site
Google Scholar
PubMed Close
,
MacKevin Ndubuisi Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia;

Search for other papers by MacKevin Ndubuisi in
Current site
Google Scholar
PubMed Close
, and
Henry S. Bishop Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia;

Search for other papers by Henry S. Bishop in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.4269/ajtmh.18-0522
Volume/Issue:
Volume 100: Issue 2
Page(s):
311–313
Restricted access
Get Permissions

Amoebic trophozoites were identified in the cervicovaginal smear of a U.S. patient without travel history at the time of intrauterine device (IUD) removal. Subsequent morphologic analysis and DNA sequencing identified a mixed cervicovaginal colonization of the female genital tract with both Entamoeba gingivalis and Entamoeba polecki in association with Actinomyces species bacteria. This highlights to the potential for colonization of the genital tract with E. gingivalis, particularly in association with IUD placement, and represents the first report of E. polecki in this context.

Author Notes

Address correspondence to Richard S. Bradbury, Parasite Diagnostics and Biology Laboratory, Centers for Disease Control and Prevention, 1600 Clifton Rd., MS D64, Atlanta, GA 30329. E-mail: isl5@cdc.gov

Conflicts of interest: R. B. has a patent Leydig 728860 removing interfering host nucleic acids for molecular parasite detection pending. R. J. and K. H. report grants from the CDC during the conduct of the study.

Authors’ addresses: Richard S. Bradbury, MacKevin Ndubuisi, and Henry S. Bishop, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, GA, E-mails: isl5@cdc.gov, nrm6@cdc.gov, and hsb2@cdc.gov. Shantanu Roy and Ibne K. Ali, Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, GA, E-mails: kwl0@cdc.gov and xzn5@cdc.gov. Jasmin R. Morrison, UnityPoint Medical Primary Care, Johnston, IA, E-mail: jasmin.morrison@unitypoint.org. Dale Waldner and Haleigh R. Delavan, UnityPoint Health, Cedar Rapids, IA, E-mails: dale.waldner@hotmail.com and haleigh.delavan@unitypoint.org. Katherine Hebbeln, Wade Aldous, and Ryan Jepson, State Hygienic Laboratory at the University of Iowa, Coralville, IA, E-mails: katherine-hebbeln@uiowa.edu, wade-aldous@uiowa.edu, and ryan-jepson@uiowa.edu.

Copyright:
© The American Society of Tropical Medicine and Hygiene 2019
Received:
23 Jun 2018
|
Accepted:
21 Jul 2018
|
Published Online:
10 Dec 2018
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Online ISSN:
1476-1645
  • 1.

    Citronberg RJ, Semel JD, 1995. Severe vaginal infection with Entamoeba histolytica in a woman who recently returned from Mexico: case report and review. Clin Infect Dis 20: 700–702.

    • Search Google Scholar
    • Export Citation
  • 2.

    Veliath AJ, Bansal R, Sankaran V, Rajaram P, Parkash S, 1987. Genital amebiasis. Int J Gynaecol Obstet 25: 249–256.

  • 3.

    Luszczak J, Bartosik M, Rzymowska J, Sochaczewska-Dolecka A, Tomaszek E, Wysokinska-Miszczuk J, Bogucka-Kocka A, 2016. The occurrence of Entamoeba gingivalis among patients with periodontal disease. Curr Issues Pharm Med Sci 29: 86–89.

    • Search Google Scholar
    • Export Citation
  • 4.

    Garcia G, Ramos F, Maldonado J, Fernandez A, Yáñez J, Hernandez L, Gaytán P, 2018. Prevalence of two Entamoeba gingivalis ST1 and ST2-kamaktli subtypes in the human oral cavity under various conditions. Parasitol Res 117: 2941–2948.

    • Search Google Scholar
    • Export Citation
  • 5.

    Beaver PC, Jung RC, Cupp EW, Craig CF, 1984. Clinical Parasitology. Philadeplphia, PA: Lea & Febiger.

  • 6.

    deMoraes-Ruehsen M, McNeill RE, Frost JK, Gupta PK, Diamond LS, Honigberg BM, 1980. Amebae resembling Entamoeba gingivalis in the genital tracts of IUD users. Acta Cytol 24: 413–420.

    • Search Google Scholar
    • Export Citation
  • 7.

    McNeill RE, Ruehsen MD, 1978. Ameba trophozoites in cervico-vaginal smears of a patient using an intrauterine device. A case report. Acta Cytol 22: 91–92.

    • Search Google Scholar
    • Export Citation
  • 8.

    Clark CG, Diamond LS, 1992. Colonization of the uterus by the oral protozoan Entamoeba gingivalis. Am J Trop Med Hyg 46: 158–160.

  • 9.

    Foda AA, El-Malky MM, 2012. Prevalence of genital tract infection with Entamoeba gingivalis among copper T 380A intrauterine device users in Egypt. Contraception 85: 108–112.

    • Search Google Scholar
    • Export Citation
  • 10.

    Royer TL, Gilchrist C, Kabir M, Arju T, Ralston KS, Haque R, Clark CG, Petri WA Jr., 2012. Entamoeba bangladeshi nov. sp., Bangladesh. Emerg Infect Dis 18: 1543–1545.

    • Search Google Scholar
    • Export Citation
  • 11.

    Stensvold CR, Lebbad M, Victory EL, Verweij JJ, Tannich E, Alfellani M, Legarraga P, Clark G, 2011. Increased sampling reveals novel lineages of Entamoeba: consequences of genetic diversity and host specificity for taxonomy and molecular detection. Protist 162: 525–541.

    • Search Google Scholar
    • Export Citation
  • 12.

    Belding DL, 1965. Textbook of Parasitology. New York, NY: Appleton Century Crofts.

  • 13.

    Stensvold CR, Winiecka–Krusnell J, Lier T, Lebbad M, 2018. Evaluation of a PCR method for detection of Entamoeba polecki, with an overview of its molecular epidemiology. J Clin Microbiol 25: e00154–18.

    • Search Google Scholar
    • Export Citation
  • 14.

    Ash LR, Orihel TC, 2007. Ash & Orihel’s Atlas of Human Parasitology. Hong Kong, China: American Society for Clinical Pathology.

  • 15.

    Verweij JJ, Polderman AM, Clark CG, 2001. Genetic variation among human isolates of uninucleated cyst-producing Entamoeba species. J Clin Microbiol 39: 1644–1646.

    • Search Google Scholar
    • Export Citation
  • 16.

    Clark CG et al. 2006. New insights into the phylogeny of Entamoeba species provided by analysis of four new small-subunit rRNA genes. Int J Syst Evol Microbiol 56: 2235–2239.

    • Search Google Scholar
    • Export Citation
  • 17.

    Boles JM, Masure O, 1986. Entamoeba polecki infection in France. Mayo Clin Proc 61: 226.

  • 18.

    Goldsmid JM, McColl D, Seaton D, 1984. Entamoeba polecki in Tasmania. Med J Aust 141: 134.

Past two years Past Year Past 30 Days
Abstract Views 109 109 15
Full Text Views 974 181 0
PDF Downloads 501 77 0
 
Membership Banner
 
 
 
Affiliate Membership Banner
 
 
Research for Health Information Banner
 
 
CLOCKSS
 
 
 
Society Publishers Coalition Banner
Save