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Use of MALDI-TOF MS for the Identification of Chad Mosquitoes and the Origin of Their Blood Meal

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  • 1 Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, IHU Méditerranée Infection, Marseille, France;
  • | 2 Department of Epidemiology of Parasitic Diseases, Malaria Research and Training Center, University of Science, Techniques and Technologies of Bamako, Bamako, Mali;
  • | 3 Aix Marseille Univ, Inserm, IRD, SESSTIM, Sciences Economiques & Sociales de la Santé & Traitement de l’Information Médicale, Marseille, France;
  • | 4 SSA, CESPA, Marseille, France
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Matrix-assisted desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a clinical microbiology tool for the systematic identification of microorganisms. It has recently been presented as an innovative tool for the rapid and accurate identification of mosquitoes and their blood meal. To evaluate the capacity of this tool to identify mosquitoes collected in a tropical environment and preserved with silica gel, we analyzed 188 mosquitoes of different species collected in Chad, which were preserved with silica gel for 2 months. The MALDI-TOF MS analysis correctly identified 96% of the mosquitoes and 37.5% of their blood meals. Using MALDI-TOF MS and molecular biology, eight mosquito species were identified, including Anopheles gambiae s.l., Anopheles rufipes, Culex quinquefasciatus, Culex neavei, Culex pipiens, Culex perexiguus, Culex rima, and Culex watti. Blood meal identification revealed that mosquitoes fed mainly on humans, birds, and cows. Matrix-assisted desorption/ionization time-of-flight mass spectrometry appears to be a promising, fast, and reliable tool to identify mosquitoes and the origin of their blood meal for samples stored with silica gel.

Author Notes

Address correspondence to Philippe Parola, Aix Marseille University, Institut de recherche pour le devéloppement (IRD), Assistance publique-Hopitaux Marseille (AP-HM), Service de Santé des Armées (SSA), Vecteurs – Infections Tropicales et Méditerranéennes (VITROME), Institut hospitalo-universitaire (IHU) Méditerranée Infection, 19-21 Blvd. Jean Moulin, Marseille 13005, France. E-mail: philippe.parola@univ-amu.fr

Financial support: The project leading to this publication has received funding from Excellence Initiative of Aix-Marseille University-A*MIDEX, a French “Investissements d’Avenir” program” (n ANR-10-IAHU-03).

Authors’ addresses: Adama Zan Diarra, Maureen Laroche, and Philippe Parola, Aix Marseille University, Institut de recherche pour le devéloppement (IRD), Assistance publique-Hopitaux Marseille (AP-HM), Service de Santé des Armées (SSA), Vecteurs – Infections Tropicales et Méditerranéennes (VITROME), Institut hospitalo-universitaire (IHU) Méditerranée Infection, Marseille, France, E-mails: adamazandiarra@gmail.com, maureen.laroche972@gmail.com, and philippe.parola@univ-amu.fr. Franck Berger, Aix Marseille University, Institut national de la santé et de la recherche médicale (INSERM), Institut de recherche pour le devéloppement (IRD), Sciences Economiques & Sociales de la Santé & Traitement de l’Information Médicale (SESSTIM), Marseille, France, Service de Santé des Armées (SSA ), Centre d’épidémiologie et de santé publique des armées (CESPA), Marseille, France, E-mail: franck1.berger@intradef.gouv.fr.

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