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-
1.
Olsen A, van Lieshout L, Marti H, Polderman T, Polman K, Steinmann P, Stothard R, Thybo S, Verweij JJ, Magnussen P, 2009. Strongyloidiasis—the most neglected of the neglected tropical diseases? Trans R Soc Trop Med Hyg 103: 967–972.
-
2.
Schar F, Trostdorf U, Giardina F, Khieu V, Muth S, Marti H, Vounatsou P, Odermatt P, 2013. Strongyloides stercoralis: global distribution and risk factors. PLoS Negl Trop Dis 7: e2288.
-
3.
Bisoffi Z et al. 2014. Diagnostic accuracy of five serologic tests for Strongyloides stercoralis infection. PLoS Negl Trop Dis 8: e2640.
-
4.
Eamudomkarn C, Sithithaworn P, Sithithaworn J, Kaewkes S, Sripa B, Itoh M, 2015. Comparative evaluation of Strongyloides ratti and S. stercoralis larval antigen for diagnosis of strongyloidiasis in an endemic area of opisthorchiasis. Parasitol Res 114: 2543–2551.
-
5.
Sultana Y, Jeoffreys N, Watts MR, Gilbert GL, Lee R, 2013. Real-time polymerase chain reaction for detection of Strongyloides stercoralis in stool. Am J Trop Med Hyg 88: 1048–1051.
-
6.
Sharifdini M, Mirhendi H, Ashrafi K, Hosseini M, Mohebali M, Khodadadi H, Kia EB, 2015. Comparison of nested polymerase chain reaction and real-time polymerase chain reaction with parasitological methods for detection of Strongyloides stercoralis in human fecal samples. Am J Trop Med Hyg 93: 1285–1291.
-
7.
Lodh N, Caro R, Sofer S, Scott A, Krolewiecki A, Shiff C, 2016. Diagnosis of Strongyloides stercoralis: detection of parasite-derived DNA in urine. Acta Trop 163: 9–13.
-
8.
Krolewiecki AJ, Koukounari A, Romano M, Caro RN, Scott AL, Fleitas P, Cimino R, Shiff CJ, 2018. Transrenal DNA-based diagnosis of Strongyloides stercoralis (Grassi, 1879) infection: Bayesian latent class modeling of test accuracy. PLoS Negl Trop Dis 12: e0006550.
-
9.
Eamudomkarn C et al. 2018. Diagnostic performance of urinary IgG antibody detection: a novel approach for population screening of strongyloidiasis. PLoS One 13: e0192598.
-
10.
Greaves D, Coggle S, Pollard C, Aliyu SH, Moore EM, 2013. Strongyloides stercoralis infection. BMJ 347: f4610.
-
11.
Koga K, Kasuya S, Khamboonruang C, Sukhavat K, Ieda M, Takatsuka N, Kita K, Ohtomo H, 1991. A modified agar plate method for detection of Strongyloides stercoralis. Am J Trop Med Hyg 45: 518–521.
-
12.
Sithithaworn P, Srisawangwong T, Tesana S, Daenseekaew W, Sithithaworn J, Fujimaki Y, Ando K, 2003. Epidemiology of Strongyloides stercoralis in north-east Thailand: application of the agar plate culture technique compared with the enzyme-linked immunosorbent assay. Trans R Soc Trop Med Hyg 97: 398–402.
-
13.
Landis JR, Koch GG, 1977. An application of hierarchical kappa-type statistics in the assessment of majority agreement among multiple observers. Biometrics 33: 363–374.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 355 | 306 | 105 |
| Full Text Views | 537 | 9 | 2 |
| PDF Downloads | 209 | 7 | 2 |
Accuracy of Urine and Serum Assays for the Diagnosis of Strongyloidiasis by Three Enzyme-Linked Immunosorbent Assay Protocols
Sirowan Ruantip
Sirowan Ruantip
Biomedical Science Program, Graduate School, Khon Kaen University, Khon Kaen, Thailand;
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Chatanun Eamudomkarn
Chatanun Eamudomkarn
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
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Anchalee Techasen
Anchalee Techasen
Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, Thailand;
Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand;
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Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand;
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Chompunoot Wangboon
Chompunoot Wangboon
Biomedical Science Program, Graduate School, Khon Kaen University, Khon Kaen, Thailand;
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
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Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
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Jiraporn Sithithaworn
Jiraporn Sithithaworn
Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand;
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Jeffrey M. Bethony
Jeffrey M. Bethony
Department of Microbiology, Immunology and Tropical Medicine, George Washington University, Washington, District of Columbia;
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Makoto Itoh
Makoto Itoh
Department of Infection and Immunology, Aichi Medical University School of Medicine, Nagakute, Japan
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Paiboon Sithithaworn
Paiboon Sithithaworn
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, Thailand;
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Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, Thailand;
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To evaluate the accuracy and reliability of urine assay for the diagnosis of strongyloidiasis, three different immunoassays were used to assess the diagnostic accuracy of anti-Strongyloides immunoglobulin G (IgG) in urine and compared with those in serum samples. Analyses by InBios enzyme-linked immunosorbent assay (ELISA) kit (recombinant NIE antigen), SciMedx ELISA kit (Strongyloides stercoralis antigen), and our in-house ELISA (Strongyloides ratti antigen) yielded comparable diagnostic performances between urine and serum assays. Levels of Strongyloides-specific IgG in urine significantly correlated with those in serum. Tests for diagnostic agreement between urine and serum IgG assays showed substantial to fair agreement (κ = 0.207–0.615). The observed quantitative and qualitative concordance between urine and serum assays in strongyloidiasis suggests that urine has similar diagnostic value to that for serum. Because of the ease and noninvasiveness of clinical sample collection, urine assay has a high potential for the initial diagnosis and mass screening of strongyloidiasis.
Author Notes
Authors’ addresses: Sirowan Ruantip and Chompunoot Wangboon, Faculty of Graduate School, KhonKaen University, Khon Kaen, Thailand, E-mails: sirowan.rtip@gmail.com and tookkiko@hotmail.com. Chatanun Eamudomkarn and Paiboon Sithithaworn, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, E-mails: paibsit@gmail.com and chatanune@yahoo.com. Anchalee Techasen, Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, Thailand, and Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand, E-mail: anchaleetechasen@gmail.com. Jiraporn Sithithaworn, Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand, E-mail: jirapornsith@gmail.com. Jeffrey M. Bethony, Department of Microbiology, Immunology and Tropical Medicine, George Washington University, Washington, DC, E-mail: jeffreybethony@gmail.com. Makoto Itoh, Department of Infection and Immunology, Aichi Medical University School of Medicine, Nagakute, Japan, E-mail: macfilaria@gmail.com.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Olsen A, van Lieshout L, Marti H, Polderman T, Polman K, Steinmann P, Stothard R, Thybo S, Verweij JJ, Magnussen P, 2009. Strongyloidiasis—the most neglected of the neglected tropical diseases? Trans R Soc Trop Med Hyg 103: 967–972.
-
2.
Schar F, Trostdorf U, Giardina F, Khieu V, Muth S, Marti H, Vounatsou P, Odermatt P, 2013. Strongyloides stercoralis: global distribution and risk factors. PLoS Negl Trop Dis 7: e2288.
-
3.
Bisoffi Z et al. 2014. Diagnostic accuracy of five serologic tests for Strongyloides stercoralis infection. PLoS Negl Trop Dis 8: e2640.
-
4.
Eamudomkarn C, Sithithaworn P, Sithithaworn J, Kaewkes S, Sripa B, Itoh M, 2015. Comparative evaluation of Strongyloides ratti and S. stercoralis larval antigen for diagnosis of strongyloidiasis in an endemic area of opisthorchiasis. Parasitol Res 114: 2543–2551.
-
5.
Sultana Y, Jeoffreys N, Watts MR, Gilbert GL, Lee R, 2013. Real-time polymerase chain reaction for detection of Strongyloides stercoralis in stool. Am J Trop Med Hyg 88: 1048–1051.
-
6.
Sharifdini M, Mirhendi H, Ashrafi K, Hosseini M, Mohebali M, Khodadadi H, Kia EB, 2015. Comparison of nested polymerase chain reaction and real-time polymerase chain reaction with parasitological methods for detection of Strongyloides stercoralis in human fecal samples. Am J Trop Med Hyg 93: 1285–1291.
-
7.
Lodh N, Caro R, Sofer S, Scott A, Krolewiecki A, Shiff C, 2016. Diagnosis of Strongyloides stercoralis: detection of parasite-derived DNA in urine. Acta Trop 163: 9–13.
-
8.
Krolewiecki AJ, Koukounari A, Romano M, Caro RN, Scott AL, Fleitas P, Cimino R, Shiff CJ, 2018. Transrenal DNA-based diagnosis of Strongyloides stercoralis (Grassi, 1879) infection: Bayesian latent class modeling of test accuracy. PLoS Negl Trop Dis 12: e0006550.
-
9.
Eamudomkarn C et al. 2018. Diagnostic performance of urinary IgG antibody detection: a novel approach for population screening of strongyloidiasis. PLoS One 13: e0192598.
-
10.
Greaves D, Coggle S, Pollard C, Aliyu SH, Moore EM, 2013. Strongyloides stercoralis infection. BMJ 347: f4610.
-
11.
Koga K, Kasuya S, Khamboonruang C, Sukhavat K, Ieda M, Takatsuka N, Kita K, Ohtomo H, 1991. A modified agar plate method for detection of Strongyloides stercoralis. Am J Trop Med Hyg 45: 518–521.
-
12.
Sithithaworn P, Srisawangwong T, Tesana S, Daenseekaew W, Sithithaworn J, Fujimaki Y, Ando K, 2003. Epidemiology of Strongyloides stercoralis in north-east Thailand: application of the agar plate culture technique compared with the enzyme-linked immunosorbent assay. Trans R Soc Trop Med Hyg 97: 398–402.
-
13.
Landis JR, Koch GG, 1977. An application of hierarchical kappa-type statistics in the assessment of majority agreement among multiple observers. Biometrics 33: 363–374.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 355 | 306 | 105 |
| Full Text Views | 537 | 9 | 2 |
| PDF Downloads | 209 | 7 | 2 |