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One hundred and ninety-five persons residing in a forested area of the Province of Darién in Panama near the Colombian border were tested for neutralizing antibodies against yellow fever, St. Louis encephalities (SLE) and Ilhéus viruses. Capacity to neutralize 2 or more logs of virus was observed in 8.4% against SLE, in 43.7% against Ilhéus and in 42% against yellow fever. The interpretation of these results is discussed.