Comparative Assay of Arthropod-Borne Group C Virus Antibodies by Tissue Culture Neutralization and Hemagglutination-Inhibition Tests

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  • Rockefeller Foundation Virus Laboratories, New York


Five viruses of the arthropod-borne group C, designated by Causey as Apeu, Caraparu, Marituba, Murutucu and Oriboca, were propagated in stable human cell lines. Each virus caused a sharp and reproducible CPE when cells were grown in a growth-promoting medium containing tryptose phosphate broth and maintained during the period of viral synthesis in Eagle's medium supplemented with calf serum at a pH of 7.2 ± 0.2.

Results of parallel HI and neutralization tests on immune mouse and rabbit sera, using a HeLa cell-virus system and a constant virus-varying serum technique for the determination of neutralizing antibodies, showed no discrepancies between the two in vitro methods when homologous systems were tested.

The experimental evidence indicates that the tissue culture neutralization test may be substituted for the mouse neutralization test or the HI test in immunological studies of the arthropod-borne group C viruses.