1921
Volume 88, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Culture-independent molecular characterization of infecting human blood specimens from a 2008 outbreak of human leptospirosis in central Sri Lanka was carried out. Of 58 quantitative real-time polymerase chain reaction-positive samples analyzed for seven multi-locus sequence typing (MLST) housekeeping genes (, , , , , , and ), interpretable data was obtained from 12 samples. Mean bacterial load was 2.2 × 10 among specimens with complete MLST profiles compared with 1.3 × 10 among specimens without complete MLST profiles; all specimens with complete profiles had at least 4.9 × 10 /mL ( = 5, < 0.001). Most (11/12) identified sequence types were ST1 ( serovar Lai) and ST44 ( serovar Geyaweera). MLST can be used to directly identify infecting strains in blood samples obtained during acute illness without the need for culture isolation, but it shows important limitations related to bacterial load.

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2017-11-20
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  • Received : 27 Aug 2012
  • Accepted : 08 Oct 2012

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