1921
Volume 88, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Anopheline specimens were collected in 2011 by human landing catch, Shannon and CDC traps from the malaria endemic localities of Santa Rosa and San Pedro in Madre de Dios Department, Peru. Most specimens were either () B or () , confirmed by polymerase chain reaction-restriction fragment length polymorphism-internal transcribed spacer 2 (PCR-RFLP-ITS2) and, for selected individuals, ITS2 sequences. A few specimens from Lupuna, Loreto Department, northern Amazonian Peru, were also identified as B. A statistical parsimony network using ITS2 sequences confirmed that all Peruvian B analyzed were identical to those in GenBank from Putumayo, southern Colombia. Sequences of the mtDNA BOLD region of specimens from all three Peruvian localities were connected using a statistical parsimony network, although there were multiple mutation steps between northern and southern Peruvian sequences. A Bayesian inference of concatenated Peruvian sequences of ITS2 + detected a single clade with very high support for all B except one individual from Lupuna that was excluded. No samples were positive for by -PCR.

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  • Received : 11 Jul 2012
  • Accepted : 23 Oct 2012

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