Volume 88, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Anopheline specimens were collected in 2011 by human landing catch, Shannon and CDC traps from the malaria endemic localities of Santa Rosa and San Pedro in Madre de Dios Department, Peru. Most specimens were either () B or () , confirmed by polymerase chain reaction-restriction fragment length polymorphism-internal transcribed spacer 2 (PCR-RFLP-ITS2) and, for selected individuals, ITS2 sequences. A few specimens from Lupuna, Loreto Department, northern Amazonian Peru, were also identified as B. A statistical parsimony network using ITS2 sequences confirmed that all Peruvian B analyzed were identical to those in GenBank from Putumayo, southern Colombia. Sequences of the mtDNA BOLD region of specimens from all three Peruvian localities were connected using a statistical parsimony network, although there were multiple mutation steps between northern and southern Peruvian sequences. A Bayesian inference of concatenated Peruvian sequences of ITS2 + detected a single clade with very high support for all B except one individual from Lupuna that was excluded. No samples were positive for by -PCR.


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  1. Instituto Nacional de Estadística e Informática (INEI), 2007. XI Censo de Poblacion y VI de Vivienda. Available at: http://www.inei.gob.pe/. Accessed June 6, 2012.
  2. da Silva-Nunes M, Moreno M, Conn JE, Gamboa D, Abeles S, Vinetz JM, Ferreira MU, , 2012. Amazonian malaria: asymptomatic human reservoirs, diagnostic challenges, environmentally driven changes in mosquito vector populations, and the mandate for sustainable control strategies. Acta Trop 121: 281291.[Crossref]
  3. December 24, 2011 epidemiological surveillance report in Peru, 2011. Bol Epidemiol 20: 10801091.
  4. Pan American Health Organization, 2010. Report on the Situation of Malaria in the Americas, 2008. Washington, DC: Pan American Health Organization.
  5. Regional Health Office of Madre de Dios, 2011. Results of malaria control and interventions in migrant populations.
  6. Aramburu Guarda J, Asayag CR, Witzig R, , 1999. Malaria reemergence in the Peruvian Amazon region. Emerg Infect Dis 5: 209215.[Crossref]
  7. Roper MH, Torres RS, Goicochea CG, Andersen EM, Guarda JS, Calampa C, Hightower AW, Magill AJ, , 2000. The epidemiology of malaria in an epidemic area of the Peruvian Amazon. Am J Trop Med Hyg 62: 247256.
  8. Fernández R, Carbajal F, Quintana J, Chauca H, Watts DM, , 1996. Presence of A. (N.) darlingi (Diptera: Culicidae), in the region surrounding Iquitos, Loreto-Peru. Bol Soc Peruana Enferm Inf Trop 5: 1020.
  9. Vittor AY, Gilman RH, Tielsch J, Glass G, Shields T, Lozano WS, Pinedo-Cancino V, Patz JA, , 2006. The effect of deforestation on the human-biting rate of Anopheles darlingi, the primary vector of Falciparum malaria in the Peruvian Amazon. Am J Trop Med Hyg 74: 311.
  10. Tineo ET, Medina CA, Fallaque SC, Chavez CL, Quispe FS, Mercado AM, Zevallos GJ, Leon CW, Palomino SM, , 2003. Geographical distribution and seasonal biting behavior of Anopheles (Nyssorhynchus) darlingi Root 1926 in Peru-Bolivia frontier localities, Madre de Dios, Peru. Rev Peru Med Exp Salud Publica 20: 7883.
  11. Flores-Mendoza C, Fernándezc R, Escobedo-Vargasc KS, Vela-Perezac Q, Schoelerbc GB, , 2004. Natural Plasmodium infections in Anopheles darlingi and Anopheles benarrochi (Diptera: Culicidae) from eastern Peru. J Med Entomol 41: 489494.[Crossref]
  12. Ruiz F, Quinones ML, Erazo HF, Calle DA, Alzate JF, Linton YM, , 2005. Molecular differentiation of Anopheles (Nyssorhynchus) benarrochi and An. (N.) oswaldoi from southern Colombia. Mem Inst Oswaldo Cruz 100: 155160.[Crossref]
  13. Quiñones ML, Ruiz F, Calle DA, Harbach RE, Erazo HF, Linton YM, , 2006. Incrimination of Anopheles (Nyssorhynchus) rangeli and An. (Nys.) oswaldoi as natural vectors of Plasmodium vivax in southern Colombia. Mem Inst Oswaldo Cruz 101: 617623.[Crossref]
  14. Reinbold-Wasson DD, Sardelis MR, Jones JW, Watts DM, Fernandez R, Carbajal F, Pecor JE, Calampa C, Klein TA, Turrel MJ, , 2012. Determinants of Anopheles seasonal distribution patterns across a forest to periurban gradient near Iquitos, Peru. Am J Trop Med Hyg 86: 459463.[Crossref]
  15. Forattini OP, , 1962. Entomologica Medica. Volume 1. Sao Paulo, Fac. Hig. Saude, Sao Paulo, Brazil.
  16. Faran ME, Linthicum KJ, , 1981. A handbook of the Amazonian species of Anopheles (Nyssorhynchus) (Diptera: Culicidae). Mosq Syst 13: 181.
  17. Consoli RA, Lourenço-de-Oliveira RL, , 1994. Major mosquito vectors in Brazil. Fundação Oswaldo Cruz, Editora Fiocruz.
  18. Hasan AU, Suguri S, Sattabongkot J, Fujimoto C, Amakawa M, Harada M, Ohmae M, , 2009. Implementation of a novel PCR based method for detecting malaria parasites from naturally infected mosquitoes in Papua New Guinea. Malar J 8: 182.[Crossref]
  19. Matson R, Tong Rios C, Banda Chavez C, Gilman RH, Florin D, Lopez Sifuentes V, Cardenas Greffa R, Peñataro Yori P, Fernandez R, Velasquez Portocarrero D, Vinetz JM, Kosek M, , 2008. Improved molecular technique for the differentiation of neotropical anopheline species. Am J Trop Med Hyg 78: 492498.
  20. Li C, Wilkerson RC, , 2007. Intragenomic rDNA ITS2 variation in the neotropical Anopheles (Nyssorhynchus) albitarsis complex (Diptera: Culicidae). J Hered 98: 5159.[Crossref]
  21. Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R, , 1994. DNA primers for amplification of mitochondrial Cytochrome c Oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol 3: 294299.
  22. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S, , 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 27312739.[Crossref]
  23. Bandelt HJ, Forster P, Röhl A, , 1999. Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol 16: 3748.[Crossref]
  24. Templeton AR, Crandall KA, Sing CF, , 1992. A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data. III. Cladogram estimation. Genetics 132: 619633.
  25. Clement M, Posada D, Crandall KA, , 2000. TCS: a computer program to estimate gene genealogies. Mol Ecol 9: 16571659.[Crossref]
  26. Ronquist F, Huelsenbeck JP, , 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 15721574.[Crossref]
  27. Marrelli MT, Floeter-Winter F, Malafronte RS, Tadei WP, Lourenço-de-Oliveira R, Flores-Mendoza C, Marinotti O, , 2005. Amazonian malaria vector anopheline relationships interpreted from ITS2 rDNA sequences. Med Vet Entomol 19: 208218.[Crossref]
  28. Cienfuegos AV, Gómez GF, Córdoba LA, Luckhart S, Conn JE, Correa MM, , 2008. Design and evaluation of methods based on PCR-RFLP of ITS2 to identify mosquitoes of Anopheles species (Diptera: Culicidae) from the Pacific coast of Colombia. Rev Biomed 19: 3544.
  29. Cienfuegos AV, Rosero DA, Naranjo N, Luckhart S, Conn JE, Correa MM, , 2011. Evaluation of a PCR-RFLP-ITS2 assay for discrimination of Anopheles species in northern and western Colombia. Acta Trop 118: 128135.[Crossref]
  30. Marrelli MT, Sallum MAM, Marinotti O, , 2006. The second internal transcribed spacer of nuclear ribosomal DNA as a tool for Latin American taxonomy: a critical review. Mem Inst Oswaldo Cruz 101: 817832.[Crossref]
  31. Sallum MAM, Marrelli MT, Nagaki SS, Laporta GZ, Dos Santos CLS, , 2008. Insight into Anopheles (Nyssorhychus) (Diptera: Culicidae) species from Brazil. J Med Entomol 45: 970981.[Crossref]

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  • Received : 11 Jul 2012
  • Accepted : 23 Oct 2012

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