Volume 87, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



A total of 30 Powassan virus (POWV) isolates from collected from Bridgeport and North Branford, CT in 2008, 2010, 2011, and 2012 and one earlier isolate from collected in Old Lyme, CT in 1978 were characterized by phylogenetic analysis of their envelope gene sequences. Powassan virus sequences segregated into two major groups termed the deer tick virus (DTV) and Powassan (POW) lineages. The lineage from was POW. The remaining viruses from grouped with the DTV lineage. Powassan viruses from Bridgeport were nearly identical and clustered with a virus strain from a human in New York. Viruses from North Branford were homogeneous and grouped with viruses from Massachusetts, northwestern Connecticut, and Ontario. These findings suggest that POWV was independently introduced into these geographical locations in Connecticut and maintained focally in their respective environments. An improved method of isolation of POWV is described.


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  • Received : 09 May 2012
  • Accepted : 13 Jul 2012
  • Published online : 03 Oct 2012

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