Volume 88, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



West Nile virus ecology has yet to be rigorously investigated in the Caribbean Basin. We identified a transmission focus in Puerto Barrios, Guatemala, and established systematic monitoring of avian abundance and infection, seroconversions in domestic poultry, and viral infections in mosquitoes. West Nile virus transmission was detected annually between May and October from 2005 to 2008. High temperature and low rainfall enhanced the probability of chicken seroconversions, which occurred in both urban and rural sites. West Nile virus was isolated from and to a lesser extent, from /, but not from the most abundant mosquito, . A calculation that combined avian abundance, seroprevalence, and vertebrate reservoir competence suggested that great-tailed grackle () is the major amplifying host in this ecosystem. West Nile virus transmission reached moderate levels in sentinel chickens during 2007, but less than that observed during outbreaks of human disease attributed to West Nile virus in the United States.


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  1. Komar N, Clark GG, , 2006. West Nile virus activity in Latin America and the Caribbean. Rev Panam Salud Publica 19: 112117.[Crossref] [Google Scholar]
  2. Hayes CG, Monath TP, , 1989. West Nile fever. , ed. The Arboviruses: Epidemiology and Ecology. Volume V. Boca Raton, FL: CRC Press, 588. [Google Scholar]
  3. Hayes EB, Komar N, Nasci RS, Montgomery SP, O'Leary DR, Campbell GL, , 2005. Epidemiology and transmission dynamics of West Nile virus disease. Emerg Infect Dis 11: 11671173.[Crossref] [Google Scholar]
  4. Komar N, Bessoff K, Diaz A, Amador MA, Young G, Perez T, Seda R, Hunsperger E, , 2012. Avian hosts of West Nile virus in Puerto Rico. Vector Borne Zoonotic Dis 12: 4754.[Crossref] [Google Scholar]
  5. Barrera R, MacKay A, Amador M, Vasquez J, Smith J, Díaz A, Acevedo V, Cabán B, Hunsperger EA, Muñoz-Jordán JL, , 2010. Mosquito vectors of West Nile virus during an epizootic outbreak in Puerto Rico. J Med Entomol 47: 11851195.[Crossref] [Google Scholar]
  6. Estrada-Franco JG, Navarro-Lopez R, Beasley DW, Coffey L, Carrara AS, Travassos da Rosa A, Clements T, Wang E, Ludwig GV, Cortes AC, Ramírez PP, Tesh RB, Barrett AD, Weaver SC, , 2003. West Nile virus in Mexico: evidence of widespread circulation since July 2002. Emerg Infect Dis 9: 16041607.[Crossref] [Google Scholar]
  7. Fernández-Salas I, Contreras-Cordero JF, Blitvich BJ, González-Rojas JI, Cavazos-Alvarez A, Marlenee NL, Elizondo-Quiroga A, Loroño-Pino MA, Gubler DJ, Cropp BC, Calisher CH, Beaty BJ, , 2003. Serologic evidence of West Nile Virus infection in birds, Tamaulipas State, México. Vector Borne Zoonotic Dis 3: 209213.[Crossref] [Google Scholar]
  8. Farfán-Ale JA, Blitvich BJ, Loroño-Pino MA, Marlenee NL, Rosado-Paredes EP, García-Rejón JE, Flores-Flores LF, Chulim-Perera L, López-Uribe M, Pérez-Mendoza G, Sánchez-Herrera I, Santamaría W, Moo-Huchim J, Gubler DJ, Cropp BC, Calisher CH, Beaty BJ, , 2004. Longitudinal studies of West Nile virus infection in avians, Yucatán State, México. Vector Borne Zoonotic Dis 4: 314.[Crossref] [Google Scholar]
  9. Morales-Betoulle ME, Morales H, Blitvich BJ, Powers AM, Davis EA, Klein R, Cordón-Rosales C, , 2006. West Nile virus in horses, Guatemala. Emerg Infect Dis 12: 10381039.[Crossref] [Google Scholar]
  10. Morales-Betoulle ME, Komar N, Martinez LE, Betoulle J-L, Alvarez D, Panella N, Contreras CL, Cordon-Rosales C, , 2008. The presence of migratory birds may reduce West Nile virus transmission in Guatemala [Spanish]. Boletín de la Universidad del Valle de Guatemala 16: 99110. [Google Scholar]
  11. Hobson-Peters J, Arévalo C, Cheah WY, Blitvich BJ, Tan CS, Sandis A, Araya LN, Hernández JL, Toye P, Hall RA, , 2011. Detection of antibodies to West Nile virus in horses, Costa Rica, 2004. Vector Borne Zoonotic Dis 11: 10811084.[Crossref] [Google Scholar]
  12. Mattar S, Komar N, Young G, Alvarez J, Gonzalez M, , 2011. Seroconversion to West Nile and St. Louis encephalitis viruses among sentinel horses, Colombia. Mem Inst Oswaldo Cruz 106: 976979.[Crossref] [Google Scholar]
  13. Bosch I, Herrera F, Navarro JC, Lentino M, Dupuis A, Maffei J, Jones M, Fernández E, Pérez N, Pérez-Emán J, Guimarães AE, Barrera R, Valero N, Ruiz J, Velásquez G, Martinez J, Comach G, Komar N, Spielman A, Kramer L, , 2007. West Nile virus, Venezuela. Emerg Infect Dis 13: 651653.[Crossref] [Google Scholar]
  14. Quirin R, Salas M, Zientara S, Zeller H, Labie J, Murri S, Lefrançois T, Petitclerc M, Martinez D, , 2004. West Nile virus, Guadeloupe. Emerg Infect Dis 10: 706708.[Crossref] [Google Scholar]
  15. Dupuis AP, 2nd Marra PP, Reitsma R, Jones MJ, Louie KL, Kramer LD, , 2005. Serologic evidence for West Nile virus transmission in Puerto Rico and Cuba. Am J Trop Med Hyg 73: 474476. [Google Scholar]
  16. Komar O, Robbins MB, Klenk K, Blitvich BJ, Marlenee NL, Burkhalter KL, Gubler DJ, Gonzalvez G, Pena CJ, Peterson AT, Komar N, , 2003. West Nile virus transmission in resident birds, Dominican Republic. Emerg Infect Dis 9: 12991302.[Crossref] [Google Scholar]
  17. Komar O, Robbins MB, Contreras GG, Benz BW, Klenk K, Blitvich BJ, Marlenee NL, Burkhalter KL, Beckett S, Gonzalvez G, Pena CJ, Peterson AT, Komar N, , 2005. West Nile virus survey of birds and mosquitoes in the Dominican Republic. Vector Borne Zoonotic Dis 5: 120126.[Crossref] [Google Scholar]
  18. Beatty ME, Hunsperger E, Long E, Schürch J, Jain S, Colindres R, Lerebours G, Bernard YM, Dobbins JG, Brown M, Clark GG, , 2007. Mosquito-borne infections after Hurricane Jeanne, Haiti, 2004. Emerg Infect Dis 13: 308310.[Crossref] [Google Scholar]
  19. Dupuis AP, 2nd Marra PP, Kramer LD, , 2003. Serologic evidence of West Nile virus transmission, Jamaica, West Indies. Emerg Infect Dis 9: 860863.[Crossref] [Google Scholar]
  20. Elizondo-Quiroga D, Davis CT, Fernandez-Salas I, Escobar-Lopez R, Velasco Olmos D, Soto Gastalum LC, Aviles Acosta M, Elizondo-Quiroga A, Gonzalez-Rojas JI, Contreras Cordero JF, Guzman H, Travassos da Rosa A, Blitvich BJ, Barrett AD, Beaty BJ, Tesh RB, , 2005. West Nile virus isolation in human and mosquitoes, Mexico. Emerg Infect Dis 11: 14491452.[Crossref] [Google Scholar]
  21. Ulloa A, Ferguson HH, Méndez-Sánchez JD, Danis-Lozano R, Casas-Martínez M, Bond JG, García-Zebadúa JC, Orozco-Bonilla A, Juárez-Ordaz JA, Farfan-Ale JA, García-Rejón JE, Rosado-Paredes EP, Edwards E, Komar N, Hassan HK, Unnasch TR, Rodríguez-Pérez MA, , 2009. West Nile virus activity in mosquitoes and domestic animals in Chiapas, México. Vector Borne Zoonotic Dis 9: 555560.[Crossref] [Google Scholar]
  22. Velásquez G, Herrera F, Komar N, Montañés H, Francisco A, Rivero J, , 2008. Evaluation of West Nile virus vectors in Venezuela, using VecTest for rapid detection of infected mosquitoes [Spanish]. Entomotropica 23: 167172. [Google Scholar]
  23. Reiter P, , 1983. A revised version of the CDC gravid mosquito trap. J Am Mosq Control Assoc 3: 325327. [Google Scholar]
  24. Clark-Gil S, Darsie RF, Jr, 1983. The mosquitoes of Guatemala, their identification, distribution and bionomics, with keys to adult females and larvae, in English and Spanish. Mosq Systematics 15: 151284. [Google Scholar]
  25. Ralph CJ, Sauer JR, Droege S, , 1995. Monitoring bird populations by point counts. Gen. Tech. Rep. PSW-GTR-149. Albany, CA: Pacific Southwest Research Station, Forest Service, U.S. Department of Agriculture.
  26. Blitvich BJ, Marlenee NL, Hall RA, Calisher CH, Bowen RA, Roehrig JT, Komar N, Langevin SA, Beaty BJ, , 2003. Epitope-blocking enzyme-linked immunosorbent assays for the detection of serum antibodies to West Nile virus in multiple avian species. J Clin Microbiol 41: 10411047.[Crossref] [Google Scholar]
  27. Beaty B, Calisher CH, Shope RE, Lennette EH, Lennette DA, Lennette ET, , 1995. Arboviruses. , eds. Viral, Rickettsial, and Chlamydial Infections. Seventh edition. Washington, DC: American Public Health Association, 189212. [Google Scholar]
  28. Morales-Betoulle ME, Monzón Pineda ML, Sosa SM, Panella N, López MR, Cordón-Rosales C, Komar N, Powers A, Johnson BW, , 2008. Culex flavivirus isolates from mosquitoes in Guatemala. J Med Entomol 6: 11871190.[Crossref] [Google Scholar]
  29. Kuno G, Chang GJ, Tsuchiya KR, Karabatsos N, Cropp CB, , 1998. Phylogeny of the genus Flavivirus. J Virol 72: 7383. [Google Scholar]
  30. Powers AM, Ledermann JP, Liu D, , 2010. Venezuelan equine encephalitis virus. , ed. Molecular Detection of Human Viral Pathogens. Boca Raton, FL: CRC Press, 373382. [Google Scholar]
  31. Lambert AJ, Lanciotti RS, , 2009. Consensus amplification and novel multiplex sequencing method for S segment species identification of 47 viruses of the Orthobunyavirus, Phlebovirus, and Nairovirus genera of the family Bunyaviridae . J Clin Microbiol 47: 23982404.[Crossref] [Google Scholar]
  32. González-Reiche AS, Monzón-Pineda M de L, Johnson BW, Morales-Betoulle ME, King N, , 2010. Detection of West Nile viral RNA from field-collected mosquitoes in tropical regions by conventional and real-time RT-PCR. , ed. RT-PCR Protocols. Second edition. New York: Springer-Walker, 109124.[Crossref] [Google Scholar]
  33. Komar N, Panella NA, Langevin SA, Brault AC, Amador M, Edwards E, Owen JC, , 2005. Avian hosts for West Nile virus in St. Tammany Parish, Louisiana 2002. Am J Trop Med Hyg 73: 10311037. [Google Scholar]
  34. Garrett-Jones C, Shidrawi GR, , 1969. Malaria vectorial capacity of a population of Anopheles gambiae: an exercise in epidemiological entomology. Bull World Health Organ 40: 531545. [Google Scholar]
  35. Hamer GL, Kitron UD, Goldberg TL, Brawn JD, Loss SR, Ruiz MO, Hayes DB, Walker ED, , 2009. Host selection by Culex pipiens mosquitoes and West Nile virus amplification. Am J Trop Med Hyg 80: 268278. [Google Scholar]
  36. Kilpatrick AM, , 2011. Globalization, land use, and the invasion of West Nile Virus. Science 334: 323327.[Crossref] [Google Scholar]
  37. Hamer GL, Chaves LF, Anderson TK, Kitron UD, Brawn JD, Ruiz MO, Loss SR, Walker ED, Goldberg TL, , 2011. Fine-scale variation in vector host use and force of infection drive localized patterns of West Nile virus transmission. PLoS ONE 6: e23767.[Crossref] [Google Scholar]
  38. Kilpatrick AM, Daszak P, Jones MJ, Marra PP, Kramer LD, , 2006. Host heterogeneity dominates West Nile virus transmission. Proc Royal Soc B-Biol Sci 273: 23272333.[Crossref] [Google Scholar]
  39. Guerrero-Sánchez S, Cuevas-Romero S, Nemeth NM, Trujillo-Olivera MT, Worwa G, Dupuis A, Brault AC, Kramer LD, Komar N, Estrada-Franco JG, , 2011. West Nile virus infection of birds, Mexico. Emerg Infect Dis 17: 22452252.[Crossref] [Google Scholar]
  40. Komar N, Dohm DJ, Turell MJ, Spielman A, , 1999. Eastern equine encephalitis in birds: relative competence of European starlings (Sturnus vulgaris). Am J Trop Med Hyg 60: 387391. [Google Scholar]
  41. Biggerstaff BJ, , 2006. PooledInfRate, version 3.0: a Microsoft Excel Add-In to Compute Prevalence Estimates from Pooled Samples. Fort Collins, CO: Centers for Disease Control and Prevention. [Google Scholar]
  42. Kramer LD, Styer LM, Ebel GD, , 2008. A global perspective on the epidemiology of West Nile virus. Annu Rev Entomol 53: 6181.[Crossref] [Google Scholar]
  43. Reisen W, Lothrop H, Chiles R, Madon M, Cossen C, Woods L, Husted S, Kramer V, Edman J, , 2004. West Nile virus in California. Emerg Infect Dis 10: 13691378.[Crossref] [Google Scholar]
  44. Tesh RB, Parsons R, Siirin M, Randle Y, Sargent C, Guzman H, Wuithiranyagool T, Higgs S, Vanlandingham DL, Bala AA, Haas K, Zerinque B, , 2004. Year-round West Nile virus activity, Gulf Coast region, Texas and Louisiana. Emerg Infect Dis 10: 16491652.[Crossref] [Google Scholar]
  45. Barrera R, Hunsperger E, Muñoz-Jordán JL, Amador M, Diaz A, Smith J, Bessoff K, Beltran M, Vergne E, Verduin M, Lambert A, Sun W, , 2008. First isolation of West Nile virus in the Caribbean. Am J Trop Med Hyg 78: 666668. [Google Scholar]
  46. Ulloa A, García-Rodríguez HH, Méndez-Sánchez JD, Danis Lozano R, Casas-Martínez M, Bond-Compean JG, García-Zebadúa J, Orozco-Bonilla A, Juárez-Ordaz JA, Farfán-Ale JA, García-Rejón J, Rosado-Paredes EP, Edwards E, Komar N, Hassan HK, Unnasch TR, Rodríguez-Pérez MA, , 2009. West Nile virus activity in mosquitoes and domestic animals in Chiapas, México. Vector Borne Zoonotic Dis 9: 555560.[Crossref] [Google Scholar]
  47. Mores CN, Turell MJ, Dohm DJ, Blow JA, Carranza MT, Quintana M, , 2007. Experimental transmission of West Nile virus by Culex nigripalpus from Honduras. Vector Borne Zoonotic Dis 7: 279284.[Crossref] [Google Scholar]
  48. Kilpatrick AM, Kramer LD, Campbell S, Alleyne EO, Dobson AP, Daszak P, , 2005. West Nile virus risk assessment and the bridge vector paradigm. Emerg Infect Dis 11: 425429.[Crossref] [Google Scholar]
  49. Goddard LB, Roth AE, Reisen WK, Scott TW, , 2002. Vector competence of California mosquitoes for West Nile virus. Emerg Infect Dis 8: 13851391.[Crossref] [Google Scholar]
  50. Turell MJ, Dohm DJ, Sardelis MR, O'Guinn ML, Andreadis TG, Blow JA, , 2005. An update on the potential of North American mosquitoes (Diptera: Culicidae) to transmit West Nile virus. J Med Entomol 42: 5762.[Crossref] [Google Scholar]
  51. Kent RJ, Crabtree MB, Miller BR, , 2010. Transmission of West Nile virus by Culex quinquefasciatus Say infected with Culex flavivirus Izabal. PLoS Negl Trop Dis 4: e671.[Crossref] [Google Scholar]
  52. Kading RC, Reiche AS, Morales-Betoulle ME, Komar N, , 2013. Host Selection of Potential West Nile Virus Vectors in Puerto Barrios, Guatemala, 2007. Am J Trop Med Hyg 88: 108115.[Crossref] [Google Scholar]
  53. Wang G, Minnis RB, Belant JL, Wax CL, , 2010. Dry weather induces outbreaks of human West Nile virus infections. BMC Infect Dis 10: 38. Available at: http://www.biomedcentral.com/1471-2334/10/38. Accessed November 20, 2011. [Crossref] [Google Scholar]
  54. Kilpatrick AM, Meola MA, Moudy RM, Kramer LD, , 2008. Temperature, viral genetics, and the transmission of West Nile virus by Culex pipiens mosquitoes. PLoS Pathog 4: e1000092.[Crossref] [Google Scholar]
  55. Reisen WK, Carroll BD, Takahashi R, Fang Y, Garcia S, Martinez VM, Quiring R, , 2009. Repeated West Nile virus epidemic transmission in Kern County, California, 2004–2007. J Med Entomol 46: 139157.[Crossref] [Google Scholar]
  56. Kilpatrick AM, Kramer LD, Jones MJ, Marra PP, Daszak P, , 2006. West Nile virus epidemics in North America are driven by shifts in mosquito feeding behavior. PLoS Biol 4: 606610.[Crossref] [Google Scholar]

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  • Received : 02 May 2012
  • Accepted : 07 Oct 2012
  • Published online : 09 Jan 2013

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