1921
Volume 87, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

In schistosomiasis elimination programs, successful discrimination of from the related animal parasites will be essential for accurate detection of human parasite transmission. Polymerase chain reaction assays employing primers from two newly selected repeated sequences, named Sh73 and Sh77, did not discriminate when amplifying Sh73-77 intra- or inter-repeats. However, amplification between Sh73 and the previously described DraI repeat exhibited discriminative banding patterns for and (sensitivity 1 pg and 10 pg, respectively). It also enabled banding pattern discrimination of and , but and did not yield amplicons. Similar inter-repeat amplification between Sh77 and DraI yielded amplicons with discriminative banding for , and ; however, was detected only at low sensitivity (1 ng). The Sh73/DraI assay detected snails infected with , , or both, and should prove useful for screening snails where discrimination of from related schistosomes is required.

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2012-12-05
2017-11-25
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  • Received : 15 Apr 2012
  • Accepted : 22 Aug 2012

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