1921
Volume 87, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Gnathostomiasis is now recognized as a zoonosis with a worldwide distribution. In the Americas, it is caused by the third-stage larvae of and in Asia mainly by . The availability and preparation of specific antigens are among the main obstacles for developing reliable immunodiagnostic tests. In this study, six immunodominant peptides were identified and characterized from , somatic antigens (AgS: 24, 32, and 40 kDa) and excretory-secretory antigens (AgES: 42, 44, and 56 kDa) by two-dimensional immunoblot analysis. Among those immunodominant peptides, two AgS spots were characterized by mass spectrometric analysis (32 kDa; pI 6.3 and 6.5) and identified as type 1 galectins. In accordance with this finding, a fraction of AgS exhibited affinity to lactose and displayed a 100% specificity and sensitivity for the diagnosis of human gnathostomiasis.

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2012-11-07
2017-11-17
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References

  1. Almeyda-Artigas RJ, Bargues MD, Mas-Coma S, , 2000. ITS-2 rDNA sequencing of Gnathostoma species (Nematoda) and elucidation of the species causing human gnathostomiasis in the Americas. J Parasitol 86: 537544.[Crossref]
  2. Díaz-Camacho SP, De la Cruz-Otero MC, Willms K, , 2000. Gnathostomosis. Revista de la Facultad de Medicina de la UNAM 43: 192201.
  3. Ishiwata K, Diaz-Camacho SP, Ogata K, Nakamura-Uchiyama F, Hiromatsu K, Nawa Y, , 2003. Evaluation of the antigenic similarities of adult-worm extracts from three Gnathostoma species, using sera from Mexican and Japanese patients with Gnathostoma infections. Ann Trop Med Parasitol 97: 629637.[Crossref]
  4. Tada I, Araki T, Matsuda H, Araki K, Akahane H, Mimori T, , 1987. A study on immunodiagnosis of gnathostomiasis by ELISA and double diffusion with special reference to the antigenicity of Gnathostoma doloresi . Southeast Asian J Trop Med Public Health 18: 444448.
  5. Waikagul J, Diaz-Camacho SP, Murrell KW, Fried B, , 2007. Gnathostomiasis. , eds. World Class Parasites: Food-Borne Parasitic Zoonoses. Fish and Plant Borne Parasites. New York: Springer-Verlag 235261.[Crossref]
  6. Diaz-Camacho SP, Willms K, de la Cruz-Otero M del C, Zazueta-Ramos ML, Baylis-Gaxiola S, Castro-Velazquez R, Osuna-Ramirez I, Bojorquez-Contreras A, Torres-Montoya EH, Sanchez-Gonzales S, , 2003. Acute outbreak of gnathostomiasis in a fishing community in Sinaloa, Mexico. Parasitol Int 52: 133140.[Crossref]
  7. Diaz-Camacho SP, Zazueta-Ramos M, Ponce-Torrecillas E, Osuna-Ramirez I, Castro-Velazquez R, Flores-Gaxiola A, Baquera-Heredia J, Willms K, Akahane H, Ogata K, Nawa Y, , 1998. Clinical manifestations and immunodiagnosis of gnathostomiasis in Culiacan, Mexico. Am J Trop Med Hyg 59: 908915.
  8. Mulvenna J, Hamilton B, Nagaraj SH, Smyth D, Loukas A, Gorman JJ, , 2009. Proteomics analysis of the excretory/secretory component of the blood-feeding stage of the hookworm Ancylostoma caninum . Mol Cell Proteomics 8: 109121.[Crossref]
  9. Morakote N, Nateewatana N, Maleewong W, Uthong T, , 1989. Anatomical localization of Gnathostoma spinigerum larval antigens by an indirect fluorescent antibody test. Southeast Asian J Trop Med Public Health 20: 291295.
  10. Rojekittikhun W, Pubampen S, , 1998. Morphological variation and abnormality of cephalic hooklets of Gnathostoma spinigerum hepatic stage larvae from laboratory infected mice. Southeast Asian J Trop Med Public Health 29: 118122.
  11. Intapan PM, Khotsri P, Kanpittaya J, Chotmongkol V, Sawanyawisuth K, Maleewong W, , 2010. Short report: immunoblot diagnostic test for neurognathostomiasis. Am J Trop Med Hyg 83: 927929.[Crossref]
  12. Kongkerd N, Uparanukraw P, Morakote N, Sajid M, McKerrow JH, , 2008. Identification and characterization of a cathepsin L-like cysteine protease from Gnathostoma spinigerum . Mol Biochem Parasitol 160: 129137.[Crossref]
  13. Laummaunwai P, Intapan PM, Wongkham C, Lulitanond V, Tayapiwatana C, Maleewong W, , 2010. Gnathostoma spinigerum: molecular cloning, expression and characterization of the cyclophilin protein. Exp Parasitol 126: 611616.[Crossref]
  14. Tapchaisri P, Nopparatana C, Chaicumpa W, Setasuban P, , 1991. Specific antigen of Gnathostoma spinigerum for immunodiagnosis of human gnathostomosis. Int J Parasitol 21: 315319.[Crossref]
  15. Uparanukraw P, Morakote N, Harnnoi T, Dantrakool A, , 2001. Molecular cloning of a gene encoding matrix metalloproteinase-like protein from Gnathostoma spinigerum . Parasitol Res 87: 751757.[Crossref]
  16. Caballero-Garcia ML, Almeyda-Artigas RJ, Mosqueda-Cabrera MA, Jimenez-Cardoso E, , 2005. Gnathostoma binucleatum: excretion-secretion antigen analysis obtained from advanced third-stage larvae in in vitro culture. Exp Parasitol 110: 140145.[Crossref]
  17. Caballero-Garcia ML, Almeyda-Artigas RJ, Mosqueda-Cabrera MA, Jimenez-Cardoso E, , 2007. Protein profile analysis from advanced third-stage larvae (AdvL3) and adult worms of Gnathostoma binucleatum (Nematoda: Spirurida). Parasitol Res 100: 555560.[Crossref]
  18. Díaz-Camacho SP, de la Cruz Otero MC, Zazueta Ramos ML, Bojórquez-Contreras Á, Sicairos-Félix J, Campista-León S, Guzmán-Loreto R, Delgado-Vargas F, León-Règagnon V, Nawa Y, , 2008. Identification of estuarine fish Dormitator latifrons as an intermediate host and Eleotris picta as a paratenic host for Gnathostoma binucleatum . Parasitol Res 106: 14211425.[Crossref]
  19. Bradford MM, , 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248254.[Crossref]
  20. Laemmli UK, Favre M, , 1973. Maturation of the head of bacteriophage T4. I. DNA packaging events. J Mol Biol 80: 575599.[Crossref]
  21. O'Farrell PH, , 1975. High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250: 40074021.
  22. Towbin H, Staehelin T, Gordon J, , 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 43504354.[Crossref]
  23. Gonzalez-Zamorano M, Mendoza-Hernandez G, Xolalpa W, Parada C, Vallecillo AJ, Bigi F, Espitia C, , 2009. Mycobacterium tuberculosis glycoproteomics based on ConA-lectin affinity capture of mannosylated proteins. J Proteome Res 8: 721733.[Crossref]
  24. Corpet F, , 1988. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res 16: 1088110890.[Crossref]
  25. Anantaphruti M, Waikagul J, Nithi-uthai S, Pubampen S, Rojekittikhun W, , 1986. Detection of humoral immune response to Gnathostoma spinigerum in mice. Southeast Asian J Trop Med Public Health 17: 172176.
  26. Wongkham C, Maleewong W, Leamviteevanich K, Intapan PM, Morakote N, , 2000. Antigenic components of Gnathostoma spinigerum recognized by infected human sera by two-dimensional polyacrylamide gel electrophoresis and immunoblotting. Asian Pac J Allergy Immunol 18: 4752.
  27. Nopparatana C, Setasuban P, Chaicumpa W, Tapchaisri P, , 1991. Purification of Gnathostoma spinigerum specific antigen and immunodiagnosis of human gnathostomiasis. Int J Parasitol 21: 677687.[Crossref]
  28. Chaicumpa W, Ruangkunaporn Y, Nopparatana C, Chongsa-Nguan M, Tapchaisri P, Setasuban P, , 1991. Monoclonal antibody to a diagnostic Mr 24,000 antigen of Gnathostoma spinigerum . Int J Parasitol 21: 735738.[Crossref]
  29. Nopparatana C, Chaicumpa W, Tapchaisri P, Setasuban P, Ruangkunaporn Y, , 1992. Towards a suitable antigen for diagnosis of Gnathostoma spinigerum infection. Int J Parasitol 22: 11511156.[Crossref]
  30. Itagaki T, Nishizaki S, Sekihashi K, Kobayashi H, Kidokoro S, Kezuka Y, Arata Y, Hirabayashi J, Kasai K, Nonaka T, , 2008. Crystallization and preliminary x-ray crystallographic analysis of galectin LEC-1 from Caenorhabditis elegans . Protein Pept Lett 15: 419422.[Crossref]
  31. Hewitson JP, Harcus YM, Curwen RS, Dowle AA, Atmadja AK, Ashton PD, Wilson A, Maizels RM, , 2008. The secretome of the filarial parasite Brugia malayi: proteomic profile of adult excretory-secretory products. Mol Biochem Parasitol 160: 821.[Crossref]
  32. Athanasiadou S, Pemberton A, Jackson F, Inglis N, Miller HR, Thevenod F, Mackellar A, Huntley JF, , 2008. Proteomic approach to identify candidate effector molecules during the in vitro immune exclusion of infective Teladorsagia circumcincta in the abomasum of sheep. Vet Res 39: 58.[Crossref]
  33. Kiel M, Josh P, Jones A, Windon R, Hunt P, Kongsuwan K, , 2007. Identification of immuno-reactive proteins from a sheep gastrointestinal nematode, Trichostrongylus colubriformis, using two-dimensional electrophoresis and mass spectrometry. Int J Parasitol 37: 14191429.[Crossref]
  34. Pou-Barreto C, Quispe-Ricalde MA, Morchon R, Vazquez C, Genchi M, Postigo I, Valladares B, Simon F, , 2008. Galectin and aldolase-like molecules are responsible for the specific IgE response in humans exposed to Dirofilaria immitis . Parasite Immunol 30: 596602.
  35. Hirabayashi J, Kasai K-i, , 1993. The family of metazoan metal-independent b-galactoside-binding lectins: structure, function and molecular evolution. Glycobiology 3: 297304.[Crossref]
  36. Álvarez-Fernández G, Bustos-Jaimes I, Castañeda-Patlán C, Guevara-Fonseca J, Romero-Álvarez I, Vázquez-Meza H, , 2010. Aspectos bioquimicos, estructurales y funcionales de las galectinas en la inmunidad. Mensaje Bioquímico, UNAM XXXIV: 1730.
  37. Yanming S, Ruofeng Y, Muleke CI, Guangwei Z, Lixin X, Xiangrui L, , 2007. Vaccination of goats with recombinant galectin antigen induces partial protection against Haemonchus contortus infection. Parasite Immunol 29: 319326.[Crossref]
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  • Received : 10 Apr 2012
  • Accepted : 29 Jul 2012

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