Volume 88, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



We evaluated infection in 397 wild specimens from five coastal localities in northern Chile by detection of minicircle DNA by polymerase chain reaction. The wild capture sites were classified into two ecotopes: a fully wild ecotope (ecotope 1) and a wild ecotope near human dwellings (ecotope 2). Infection rates varied between 11% and 27%. Minicircle hybridization assays showed that lineages Tc II and Tc VI were commonly detected in ecotope 1 and ecotope 2, respectively. These results are discussed in the context of insect proximity to human dwellings, the alimentary profile of sp., lineages detected in the past in the same disease-endemic area circulating in humans, and .


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  1. Lent H, Wygodzinsky P, , 1979. Revision of the triatominae (Hemiptera: Reduviidae) and their significance as vectors of Chagas disease. Bull Am Mus Nat Hist 163: 130138. [Google Scholar]
  2. Zingales B, Andrade SG, Briones MR, Campbell DA, Chiari E, Fernandes O, Guhl F, Lage Silva E, Macedo AM, Machado CR, Miles MA, Romanha AJ, Sturm N, Tibayrenc M, Schijman AG, , 2009. A new consensus for Trypanosome cruzi intraspecific nomenclature second revision meeting recommend TcI to TcVI. Mem Inst Oswaldo Cruz 104: 10511054.[Crossref] [Google Scholar]
  3. Avila HA, Simpson L, , 1995. Organization and complexity of minicircle-encoded guide RNAs in Trypanosoma cruzi . RNA 1: 939947. [Google Scholar]
  4. Veas F, Breniere SF, Cuny G, Brengues C, Solari A, Tibayrenc M, , 1991. General procedure to construct highly specific kDNA probes for clones of Trypanosoma cruzi for sensitive detection by polymerase chain reaction. Cell Mol Biol 37: 7384. [Google Scholar]
  5. Lent H, Jurberg J, Galvão C, , 1994. Revalidação do gênero Mepraia, Mazza, Gajardo & Jorg, 1940 (Hemiptera, Reduviidae, Triatominae). Mem Inst Oswaldo Cruz 89: 347352.[Crossref] [Google Scholar]
  6. WHO, 2000. Chagas disease: interruption of transmission in Chile. Wkly Epidemiol Rec 75: 1012. [Google Scholar]
  7. Botto-Mahan C, Cattan PE, Canals M, Acuña M, , 2005. Seasonal variation in the home range and host availability of the blood-sucking insect Mepraia spinolai in wild environment. Acta Trop 95: 160163.[Crossref] [Google Scholar]
  8. Frías DA, Henry AA, González CR, , 1998. Mepraia gajardoi: a new species of Triatominae (Hemiptera: Reduviidae) from Chile and its comparison with Mepraia spinolai . Rev Chil Hist Nat 71: 177188. [Google Scholar]
  9. Campos R, Botto-Mahan C, Coronado X, Catala S, Solari A, , 2013. Phylogenetic relationships of the Spinolai complex and other Triatomini based on mitochondrial DNA sequences (Hemiptera: Reduvidae). Vector Borne Zoonotic Dis 13: 7376.[Crossref] [Google Scholar]
  10. Sagua H, Araya J, González J, Neira I, , 2000. Mepraia spinolai in the southeastern Pacific Ocean coast (Chile): first insular record and feeding pattern on the Pan de Azúcar Island. Mem Inst Oswaldo Cruz 95: 167170.[Crossref] [Google Scholar]
  11. Wincker P, Britto C, Pereira JB, Cardoso MA, Oeleman O, Morel CM, , 1994. Use of a simplified polymerase chain reaction procedure to detect Trypanosoma cruzi in blood samples from chronic chagasic patients in a rural endemic area. Am J Trop Med Hyg 51: 771777. [Google Scholar]
  12. di Castri F, Hajek ER, , 1976. Bioclimatología de Chile. Santiago, Chile: Ediciones de la Universidad Católica de Chile. [Google Scholar]
  13. Ortiz JC, , 1980. Estudios comparativos de algunas poblaciones de Tropidurus de la costa Chilena. Ann Mus His Nat 13: 267280. [Google Scholar]
  14. Kollien A, Schaub G, , 1999. Development of Trypanosoma cruzi after starvation and feeding of the vector: a review. Tokai J Exp Clin Med 23: 335340. [Google Scholar]
  15. Schenome H, , 1999. Xenodiagnosis. Mem Inst Oswaldo Cruz 94: 289294.[Crossref] [Google Scholar]
  16. Sokal R, Rohlf FJ, , 1995. Biometry: The Principles and Practice of Statistics in Biological Research. New York: WH Freeman and Company. [Google Scholar]
  17. Coronado X, Rozas M, Botto-Mahan C, Ortiz S, Apt W, Cattan P, Solari A, , 2009. Molecular epidemiology of Chagas disease in the wild transmission cycle: the evolution in the sylvatic vector Mepraia spinolai from an endemic area of Chile. Am J Trop Med Hyg 81: 656659.[Crossref] [Google Scholar]
  18. Kierszenbaum F, Ivanyi J, Budzko DB, , 1976. Mechanisms of natural resistance to trypanosomal infection. Role of complement in avian resistance to Trypanosoma cruzi infection. Immunol 30: 16. [Google Scholar]
  19. Teixeira ARL, Nascimento RJ, Sturm NR, , 2006. Evolution and pathology in Chagas disease: a review. Mem Inst Oswaldo Cruz 101: 463491.[Crossref] [Google Scholar]
  20. Cardinal MV, Lauricella MA, Ceballos LA, Lanati L, Marcet PL, Levin MJ, Kitron U, Gürtler RE, Schijman AG, , 2008. Molecular epidemiology of domestic and sylvatic Trypanosoma cruzi infection in rural northwestern Argentina. Int J Parasitol 38: 15331543.[Crossref] [Google Scholar]
  21. Ordenes H, Ehrenfeld M, Cattan PE, Canals M, , 1996. Infección tripano-triatomina de Triatoma spinolai en una zona de riesgo epidemiológico. Rev Med Chil 124: 10531057. [Google Scholar]
  22. Solari A, Wallace A, Ortiz S, Venegas J, Sanchez G, , 1998. Biological characterization of Trypanosoma cruzi stocks from Chilean insect vectors. Exp Parasitol 89: 312322.[Crossref] [Google Scholar]
  23. Gonzalez J, Muñoz S, Ortiz S, Anacona D, Salgado S, Galleguillos M, Neira I, Sagua H, Solari A, , 1995. Biochemical, immunological, and biological characterization of Trypanosoma cruzi populations of the Andean north of Chile. Exp Parasitol 81: 125135.[Crossref] [Google Scholar]
  24. Torres JP, Ortiz S, Muñoz S, Solari A, , 2004. Trypanosoma cruzi isolates from Chile are heterogeneous and composed of mixed populations when characterized by schizodeme and southern analyses. Parasitol 128: 161168.[Crossref] [Google Scholar]
  25. Arribada AC, Apt W, Aguilera X, Solari A, Arribada AM, Sandoval J, , 1990. Cardiopatía chagásica en la primera región de Chile. Estudio clínico, epidemiológico y parasicológico. Rev Med Chil 118: 846854. [Google Scholar]
  26. Diez C, Lorenz V, Ortiz S, Gonzalez V, Racca A, Bontempi I, Manattini S, Solari A, Marcipar I, , 2010. Genotyping of Trypanosoma cruzi sublineages in human samples from a northeast Argentina area by hybridization with DNA probes and specific polymerase chain reaction (PCR). Am J Trop Med Hyg 82: 6773.[Crossref] [Google Scholar]
  27. Botto-Mahan C, Sepulveda M, Vidal M, Acuña M, Ortiz S, Solari A, , 2008. Trypanosoma cruzi infection in the sylvatic kissing bug Mepraia gajardoi from the Chilean southern Pacific Ocean coast. Acta Trop 105: 166169.[Crossref] [Google Scholar]

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  • Received : 09 Apr 2012
  • Accepted : 03 Nov 2012
  • Published online : 06 Feb 2013

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