Volume 88, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



We previously described loop-mediated isothermal amplification (LAMP) for detection of and DNA in infected snails. In the present study, we adapted the LAMP assay for application in field laboratories in schistosomiasis-endemic areas. Isolation of DNA was simplified by blotting snail tissue (extracted in NaOH/sodium dodecyl sulfate) onto treated membranes, which enabled preservation at ambient temperatures. A ready-mix of LAMP reagents, suitable for shipment at ambient temperature and storage in minimal refrigeration, was used. Local survey teams without experience in molecular biology acquired operational expertise with this test within a few hours. Fifty-four field-caught snails were tested locally by LAMP and 59 were tested at similar conditions in Jerusalem. The LAMP results were consistent with those of a polymerase chain reaction; only four samples showed false-negative results. Results indicate that LAMP assays are suitable for detection of and in low-technology parasitology laboratories in which schistosomiasis elimination activities are undertaken.


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  1. Wang LD, Guo G, Wu XH, Chen HG, Wang TP, Zhu SP, Zhang ZH, Steinmann P, Yang GJ, Wang SP, Wu ZD, Wang LY, Hao Y, Bergquist R, Utzinger J, Zhou XN, , 2009. China's new strategy to block Schistosoma japonicum transmission: experiences and impact beyond schistosomiasis. Trop Med Int Health 14: 14751483.[Crossref] [Google Scholar]
  2. Spear RC, Seto EY, Carlton EJ, Liang S, Remais JV, Zhong B, Qiu D, , 2011. The challenge of effective surveillance in moving from low transmission to elimination of schistosomiasis in China. Int J Parasitol 41: 12431247.[Crossref] [Google Scholar]
  3. Hamburger J, Hoffman O, Kriuki HC, Muchiri EM, Ouma JH, Koech DK, Sturrock RF, King CH, , 2004. Large-scale, polymerase chain reaction-based surveillance of Schistosoma haematobium DNA in snails from transmission sites in coastal Kenya: a new tool for studying the dynamics of snail infection. Am J Trop Med Hyg 71: 765773. [Google Scholar]
  4. King CH, Sturrock RF, Kariuki CH, Hamburger J, , 2006. Transmission control for schistosomiasis—why it matters now. Trends Parasitol 22: 575582.[Crossref] [Google Scholar]
  5. Hamburger J, He N, Xin X, Ramzy RM, Jourdane J, Ruppel A, , 1998. A polymerase chain reaction assay for detecting snails infected with bilharzias parasites (Schistosoma mansoni) from very early prepatency. Am J Trop Med Hyg 59: 872876. [Google Scholar]
  6. Hamburger J, He N, Abbasi I, Ramzi RM, Jourdane J, Ruppel A, , 2001. Polymerase chain reaction assay bnased on highly repeated sesequence of Schistosoma haematobium: a potential tool for monitoring schistosome infested water. Am J Trop Med Hyg 65: 907911. [Google Scholar]
  7. Abbasi I, King CH, Muchiri EM, Hamburger J, , 2010. Detection of Schistosoma mansoni and Schistosoma haematobium DNA by loop-mediated isothermal amplification: identification of infected snails from early prepatency. Am J Trop Med Hyg 83: 427432.[Crossref] [Google Scholar]
  8. Kumagai T, Furoshima-Shimogawara R, Ohmae H, Wang P, Lu S, Chen R, Wen L, Ohta N, , 2010. Detection of early and single infections of Schistosoma japonicum in the intermediate host snail, Oncomelania hupensis by PCR and loop-mediated isothermal amplification (LAMP) assay. Am J Trop Med Hyg 83: 542548.[Crossref] [Google Scholar]
  9. Xu J, Rong R, Zhang HQ, Shi CJ, Zhu XQ, Xia CM, , 2010. Sensitive and rapid detection of Schistosoma japonicum DNA by loop-mediated isothermal amplification (LAMP). Int J Parasitol 40: 327331.[Crossref] [Google Scholar]
  10. World Health Organization, 2012. Third STAG Global Working Group Meeting on Monitoring and Evaluation of Preventive Chemotherapy. Summary Points and Recommendations. Geneva: World Health Organization. [Google Scholar]
  11. Webster BL, Rollinson D, Stothard JR, Huyse T, , 2010. Rapid diagnostic multiplex PCR (RD-PCR) to discriminate Schistosoma haematobium and S. bovis . J Helminthol 84: 107114.[Crossref] [Google Scholar]
  12. Kane RA, Stothard JR, Rollinson D, Leclipteux T, Evraerts J, Sanderley CJ, Allan F, Betson M, Kaba R, Mertens P, Laurent T, , 2011. Detection and quantification of schistosome DNA in freshwater snails using either fluorescent probes in real-time PCR or oligochromatographic dipstick assays targeting the ribosomal intergenic spacer. Acta Trop [Epub ahead of print]. [Google Scholar]
  13. Abbasi I, King CH, Sturrock RF, Kariuki C, Muchiri E, Hamburger J, , 2007. Differentiation of Schistosoma haematobium from related schistosomes by PCR amplifying an inter-repeat sequence. Am J Trop Med Hyg 76: 950955. [Google Scholar]
  14. Mori Y, Kitao M, Tomita N, Notomi T, , 2004. Real-time turbidimetry of LAMP reaction for quantifying template DNA. J Biochem Biophys Methods 59: 145157.[Crossref] [Google Scholar]
  15. Neonakis IK, Spandidos DA, Petinaki E, , 2011. Use of loop mediated isothermal amplification of DNA for the rapid detection of Micobacterium tuberculosis in clinical cases. Eur J Microbiol Infect Dis 30: 937942.[Crossref] [Google Scholar]
  16. Satayathum SA, Muchiri EM, Ouma JH, Whalen CC, King CH, , 2006. Factors affecting infection or reinfection with Schistosoma haematobium in coastal Kenya: Survival analysis during a nine-year school-based treatment. Am J Trop Med Hyg 75: 8392. [Google Scholar]
  17. Clennon JA, Mungai PL, Muchiri EM, King CH, Kitron U, , 2006. Spatial and temporal variations in local transmission of Schistosoma haematobium in Msambweni, Kenya. Am J Trop Med Hyg 75: 10341041. [Google Scholar]

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  • Received : 01 Apr 2012
  • Accepted : 07 Oct 2012
  • Published online : 06 Feb 2013

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