Volume 87, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



In 2005, sulphadoxine-pyrimethamine (SP) became the drug of choice for intermittent preventive treatment of malaria in pregnancy (IPTp) in Ghana. Reports suggest the use of SP by others to treat uncomplicated malaria. Because of the increased use of SP, the prevalence of mutations in the genes, dihydrofolate reductase (), and dihydropteroate synthetase (), linked to SP resistance in were determined. Blood samples from 945 children with uncomplicated malaria collected at nine sites from 2003 to 2010 were analyzed using polymerase chain reaction and restriction fragment length polymorphism. Prevalence of the triple and plus quadruple mutations showed significant increase in trend from 2003 to 2010 (χ = 18.78, < 0.001, χ = 15.11, < 0.001, respectively). For double mutant G437 + E540 the prevalence was low (1.12%) caused by the very low prevalence of E540. Our findings show the wide use of SP in Ghana and therefore its use for IPTp needs to be closely monitored.


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  • Received : 30 Mar 2012
  • Accepted : 06 Aug 2012
  • Published online : 05 Dec 2012

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