1921
Volume 87, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

We have previously developed a polymerase chain reaction (PCR) assay for detection of infection, which proved very sensitive and specific for identification of infected dogs. We have now developed a loop-mediated isothermal amplification (LAMP) assay, which amplifies the same genomic repeated sequences of for coprodetection. This assay enabled detection of a single egg in fecal samples and showed high species specificity for with no cross-amplification of DNA from closely related helminths, including . Because the method does not require thermocycling for DNA amplification, or electrophoresis for amplicon detection, it can potentially be used for identification of -infected dogs to enable large-scale surveys in endemic countries where highly specialized equipment to undertake PCR analysis is rare.

Loading

Article metrics loading...

The graphs shown below represent data from March 2017
/content/journals/10.4269/ajtmh.2012.12-0184
2012-11-07
2020-07-16
Loading full text...

Full text loading...

/deliver/fulltext/14761645/87/5/883.html?itemId=/content/journals/10.4269/ajtmh.2012.12-0184&mimeType=html&fmt=ahah

References

  1. WHO, 2001. WHO/OIE Manual on Echinococcosis in Humans and Animals: a Public Health Problem of Global Concern. Eckert J, Gemmel MA, Meslin FX, Pawlowski ZS, eds. Geneva: World Health Organization, 1263.
    [Google Scholar]
  2. Eckert J, Conraths FJ, Tackmann K, 2000. Echinococcosis: an emerging or re-emerging zoonosis? Int J Parasitol 30: 12831294.[Crossref]
    [Google Scholar]
  3. Craig PS, Rogan MT, Allan JC, 1996. Detection, screening and community epidemiology of taeniid cestode zoonoses: cystic echinococcosis, alveolar echinococcosis and neurocysticercosis. Adv Parasitol 38: 169250.[Crossref]
    [Google Scholar]
  4. Allan JC, Craig PS, Garcia Novel J, Mencos F, Liu D, Wang Y, Wen H, Zhou P, Stringer R, Rogan M, Zeyhle E, 1992. Coproantigen detection for immunodiagnosis of echinococcosis and taeniasis in dogs and humans. Parasitology 104: 347356.[Crossref]
    [Google Scholar]
  5. Deplazes P, Jimenez-Palacios S, Gottstein B, Skaggs J, Eckert J, 1994. Detection of Echinococcus coproantigens in stray dogs of northern Spain. Appl Parasitol 35: 297301.
    [Google Scholar]
  6. Malgor R, Nonaka N, Basmadjian I, Sakai H, Carambula B, Oku Y, Carmona C, Kamiya M, 1997. Coproantigen detection in dogs experimentally and naturally infected with Echinococcus granulosus by a monoclonal antibody-based enzyme–linked immunosorbent assay. Int J Parasitol 27: 16051612.[Crossref]
    [Google Scholar]
  7. Moro PL, Bonifacia N, Gilman RH, Lopera L, Silva B, Takumoto R, Verastegui M, Cabrera L, 1999. Field diagnosis of Echinococcus granulosus infection among intermediate and definitive hosts in an endemic focus of human cystic echinococcosis. Trans R Soc Trop Med Hyg 93: 611615.[Crossref]
    [Google Scholar]
  8. Casaravilla C, Malgor R, Rossi A, Sakai H, Nonaka N, Kamiya M, Carmona C, 2005. Production and characterization of monoclonal antibodies against excretory/secretory products of adult Echinococcus granulosus, and their application to co-proantigen detection. Parasitol Int 54: 4349.[Crossref]
    [Google Scholar]
  9. Štefanić S, Shaikenov BS, Deplazes P, Dinkel A, Torgerson PR, Mathis A, 2004. Polymerase chain reaction for detection of patent infections of Echinococcus granulosus (“sheep strain”) in naturally infected dogs. Parasitol Res 92: 347351.[Crossref]
    [Google Scholar]
  10. Dinkel A, Njoroge EM, Zimmermann A, Wälz M, Zeyhle E, Elmahdi IE, Mackenstedt U, Romig T, 2004. A PCR system for detection of species and genotypes of the Echinococcus granulosus-complex, with reference to the epidemiological situation in eastern Africa. Int J Parasitol 34: 645653.[Crossref]
    [Google Scholar]
  11. Abbasi I, Branzburg A, Campos-Ponce M, Abdel Hafez SK, Raoul F, Craig PS, Hamburger J, 2003. Coprodiagnosis of Echinococcus granulosus infection in dogs by amplification of a newly identified repeated DNA sequence. Am J Trop Med Hyg 69: 324330.
    [Google Scholar]
  12. Boufana BS, Campos-Ponce M, Naidich A, Buishi I, Lahmar S, Zeyhle E, Jenkins DJ, Combes B, Wen H, Xiao N, Nakao M, Ito A, Qiu J, Craig PS, 2008. Evaluation of three PCR assays for the identification of the sheep strain (genotype 1) of Echinococcus granulosus in canid feces and parasite tissues. Am J Trop Med Hyg 78: 777783.
    [Google Scholar]
  13. Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T, 2000. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28: E63.[Crossref]
    [Google Scholar]
  14. Wastling SL, Picozzi K, Kakembo AS, Welburn SC, 2010. LAMP for human African trypanosomiasis: a comparative study of detection formats. PLoS Negl Trop Dis 4: E865.[Crossref]
    [Google Scholar]
  15. Iseki H, Kawai S, Takahashi N, Hirai M, Tanabe K, Yokoyama N, Igarashi I, 2010. Evaluation of a loop-mediated isothermal amplification method as a tool for diagnosis of infection by the zoonotic simian malaria parasite Plasmodium knowlesi . J Clin Microbiol 48: 25092514.[Crossref]
    [Google Scholar]
  16. Muller H, Aysul N, Liu Z, Salih DA, Karagenc T, Beyer D, Kullmann B, Ahmed JS, Seitzer U, 2010. Development of a loop-mediated isothermal amplification (LAMP) assay for rapid diagnosis of Babesia canis infections. Transbound Emerg Dis 57: 6365.[Crossref]
    [Google Scholar]
  17. Aonuma H, Yoshimura A, Perera N, Shinzawa N, Bando H, Oshiro S, Nelson B, Fukumoto S, Kanuka H, 2009. Loop-mediated isothermal amplification applied to filarial parasites detection in the mosquito vectors: Dirofilaria immitis as a study model. Parasit Vectors 2: 215.[Crossref]
    [Google Scholar]
  18. Abbasi I, King CH, Muchiri EM, Hamburger J, 2010. Detection of Schistosoma mansoni and Schistosoma haematobium DNA by loop-mediated isothermal amplification: identification of infected snails from early prepatency. Am J Trop Med Hyg 83: 427432.[Crossref]
    [Google Scholar]
  19. Mathis A, Deplazes P, 2006. Copro-DNA tests for diagnosis of animal taeniid cestodes. Parasitol Int 55: 587590.[Crossref]
    [Google Scholar]
  20. Naidich A, McManus DP, Canova SG, Gutierrez AM, Zhang W, Guarnera EA, Rosenzvit MC, 2006. Patent and pre-patent detection of Echinococcus granulosus genotypes in the definitive host. Mol Cell Probes 20: 110.[Crossref]
    [Google Scholar]
  21. Geojith G, Dhanasekaran S, Chandran SP, Kenneth J, 2011. Efficacy of loop mediated isothermal amplification (LAMP) assay for the laboratory identification of Mycobacterium tuberculosis isolates in a resource limited setting. J Microbiol Methods 84: 7173.[Crossref]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2012.12-0184
Loading
/content/journals/10.4269/ajtmh.2012.12-0184
Loading

Data & Media loading...

Erratum

  • Received : 22 Mar 2012
  • Accepted : 17 Jul 2012
  • Published online : 07 Nov 2012
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error