Volume 87, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



We have previously developed a polymerase chain reaction (PCR) assay for detection of infection, which proved very sensitive and specific for identification of infected dogs. We have now developed a loop-mediated isothermal amplification (LAMP) assay, which amplifies the same genomic repeated sequences of for coprodetection. This assay enabled detection of a single egg in fecal samples and showed high species specificity for with no cross-amplification of DNA from closely related helminths, including . Because the method does not require thermocycling for DNA amplification, or electrophoresis for amplicon detection, it can potentially be used for identification of -infected dogs to enable large-scale surveys in endemic countries where highly specialized equipment to undertake PCR analysis is rare.


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  • Received : 22 Mar 2012
  • Accepted : 17 Jul 2012
  • Published online : 07 Nov 2012

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