Volume 88, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



We tested a prototype stool enzyme-linked immunosorbent assay (ELISA) () that is simultaneously diagnostic for , , and in a rural pediatric clinic in Guatemala. We compared its results to those of three individual ELISAs for these parasites, assessed the prevalence of these parasites, and compared our findings to those found by stool microscopy. We tested 620 non-diarrheal stools. The diagnosed 57 positive samples and 52 (91%) had a correlating positive result in an individual assay, giving a kappa coefficient of 0.90. spp., , and spp. were detected in 52 (8.4%), 2 (0.3%), and 3 (0.5%) samples, respectively. Twenty-three (40%) samples positive by ELISA for spp. were identified by microscopy. This study is the first to test the in this setting and, to our knowledge, represents the first assessment of these parasites in Guatemala by stool ELISA.


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  • Received : 03 Mar 2012
  • Accepted : 16 Oct 2012
  • Published online : 09 Jan 2013

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