1921
Volume 87, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Our objective was to identify local animal reservoirs of leptospirosis to explain the unusual features of strains recently described among patients on the island of Mayotte. By means of a microscopic agglutination test using local clinical isolates, we found that 11.2% of black rats were seropositive to , whereas 10.2% of flying foxes, 2% of lemurs, 93.1% of domestic dogs, and 87.5% of stray dogs were seropositive. As observed in humans, Mini was the main serogroup circulating in animals, whereas serogroup Icterohaemorrhagiae was absent. Using quantitative polymerase chain reaction, we also showed that 29.8% of rats carried leptospires in their kidneys. The sequencing of 16S rRNA gene sequences of found in black rat kidneys identified four genomospecies (, , and group B), which established black rats as the major source of leptospirosis transmission to humans. The origins of such a genetic diversity in strains are discussed.

Loading

Article metrics loading...

The graphs shown below represent data from March 2017
/content/journals/10.4269/ajtmh.2012.12-0102
2012-07-02
2018-12-16
Loading full text...

Full text loading...

/deliver/fulltext/14761645/87/1/134.html?itemId=/content/journals/10.4269/ajtmh.2012.12-0102&mimeType=html&fmt=ahah

References

  1. McBride AJ, Athanazio DA, Reis MG, Ko AI, , 2005. Leptospirosis. Curr Opin Infect Dis 18: 376386.[Crossref] [Google Scholar]
  2. Levett PN, , 2001. Leptospirosis. Clin Microbiol Rev 14: 296326.[Crossref] [Google Scholar]
  3. Bourhy P, Collet L, Lernout T, Zinini F, Hartskeerl RA, van der Linden H, Thiberge JM, Diancourt L, Brisse S, Giry C, Pettinelli F, Picardeau M, , 2012. Human Leptospira isolates circulating in Mayotte (Indian Ocean) have unique serological and molecular features. J Clin Microbiol 50: 307311.[Crossref] [Google Scholar]
  4. Bourhy P, Collet L, Clément S, Huerre M, Ave P, Giry C, Pettinelli F, Picardeau M, , 2010. Isolation and characterization of new Leptospira genotypes from patients in Mayotte (Indian Ocean). PLoS Negl Trop Dis 4: e724.[Crossref] [Google Scholar]
  5. Desvars A, Duval T, Punelle C, Pascal M, Vourc'h G, , 2009. The flying-fox Pteropus seychellensis in Mayotte (Comoros): capture and blood sample methods. J Wildl Dis 45: 870873.[Crossref] [Google Scholar]
  6. West G, Heard DJ, Caulkett N, , 2007. Zoo Animal and Wildlife Immobilization and Anesthesia. Ames, IA: Wiley-Blackwell.[Crossref] [Google Scholar]
  7. Parliament and the Council of the European Union Union E, 2010. Directive 2010/63/UE of the European Parliament and Council of September 22, 2010, relative to the protection of animals used for scientific purposes. , ed. Official Journal, L 276/33-L 276/79 .
  8. Faine S, Adler B, Bolin C, Pérolat P, , 1999. Leptospira and Leptospirosis. Melbourne, Australia: MediSci. [Google Scholar]
  9. Kwok-Keung K, Meiyun Wu S, Morin Nissom P, Oh SK, Choo AB, , 2008. Generation of high-level stable transgene expressing human embryonic stem cell lines using Chinese hamster elongation factor-1alpha promoter system. Stem Cells Dev 17: 825836.[Crossref] [Google Scholar]
  10. Stoddard RA, Gee JE, Wilkins PP, McCaustland K, Hoffmaster AR, , 2009. Detection of pathogenic Leptospira spp. through TaqMan polymerase chain reaction targeting the LipL32 gene. Diagn Microbiol Infect Dis 64: 247255.[Crossref] [Google Scholar]
  11. Mérien F, Amouriaux P, Pérolat P, Baranton G, Saint-Girons I, , 1992. Polymerase chain reaction for detection of Leptospira spp. in clinical sample. J Clin Microbiol 30: 22192224. [Google Scholar]
  12. Postic D, Riquelme-Sertour N, Merien F, Pérolat P, Baranton G, , 2000. Interest of partial 16S rDNA gene sequences to resolve heterogeneities between Leptospira collections: application to L. meyeri . Res Microbiol 151: 333341.[Crossref] [Google Scholar]
  13. Soubeyran Y, , 2008. Espèces Exotiques Envahissantes dans les Collectivités Françaises d’Outre-Mer. Etats des Lieux et Recommandations. Synthèse par Collectivité. Paris, France: Comité français de l’UICN. [Google Scholar]
  14. Smythe LD, Field HE, Barnett LJ, Smith CS, Dohnt MF, Symonds ML, Moore MR, Rolfe PF, , 2002. Leptospiral antibodies in flying foxes in Australia. J Wildl Dis 38: 182186.[Crossref] [Google Scholar]
  15. Cox TE, Smythe LD, Leung LKP, , 2005. Flying foxes as carrier of pathogenic Leptospira species. J Wildl Dis 41: 753757.[Crossref] [Google Scholar]
  16. O'Brien J, , 2011. Bats of the western Indian Ocean islands. Animals 1: 259290.[Crossref] [Google Scholar]
  17. Tulsiani SM, Cobbold RN, Graham GC, Dohnt MF, Burns M-A, Leung LK-P, Field HE, Smythe LD, Craig SB, , 2011. The role of fruit bats in the transmission of pathogenic leptospires in Australia. Ann Trop Med Parasitol 105: 7184.[Crossref] [Google Scholar]
  18. Baulu J, Everard CO, Everard JD, , 1987. Leptospires in vervet monkeys (Cercopithecus aethiops sabaeus) on Barbados. J Wildl Dis 23: 6066.[Crossref] [Google Scholar]
  19. Minette HP, Shaffer MF, , 1968. Experimental leptospirosis in monkeys. Am J Trop Med Hyg 17: 202212. [Google Scholar]
  20. Szonyi B, Agudelo-Flórez P, Ramírez M, Moreno N, Ko AI, , 2011. An outbreak of severe leptospirosis in capuchin (Cebus) monkeys. Vet J 188: 237239.[Crossref] [Google Scholar]
  21. Perolat P, Poingt J-P, Vie J-C, Jouaneau C, Baranton G, Gysin J, , 1992. Occurrence of severe leptospirosis in a breeding colony of squirrel monkeys. Am J Trop Med Hyg 46: 538545. [Google Scholar]
  22. Pereira MM, Da Silva JJ, Pinto MA, Da Silva MF, Machado MP, Lenzi HL, Marchevsky RS, , 2005. Experimental leptospirosis in marmoset monkeys (Callithrix jacchus): a new model for studies of severe pulmonary leptospirosis. Am J Trop Med Hyg 72: 1320. [Google Scholar]
  23. Sykes JE, Hartmann K, Lunn KF, Moore GE, Stoddard RA, Goldstein RE, , 2010. 2010 ACVIM small animal consensus statement on leptospirosis: diagnosis, epidemiology, treatment, and prevention. J Vet Intern Med 25: 113.[Crossref] [Google Scholar]
  24. Levett PN, , 2003. Usefulness of serologic analysis as a predictor of the infecting serovar in patients with severe leptospirosis. Clin Infect Dis 36: 447452.[Crossref] [Google Scholar]
  25. Smythe LD, Wuthiekanun V, Chierakul W, Suputtamongkol Y, Tiengrim S, Dohnt MF, Symonds ML, Slack AT, Apiwattanaporn A, Chueasuwanchai S, Day NP, Peacock SJ, , 2009. The microscopic agglutination test (MAT) is an unreliable predictor of infecting Leptospira serovar in Thailand. Am J Trop Med Hyg 81: 695697.[Crossref] [Google Scholar]
  26. Brod CS, Aleixo JA, Jouglard SD, Fernandes CP, Teixeira JL, Dellagostin OA, , 2005. Evidence of dog as a reservoir for human leptospirosis: a serovar isolation, molecular characterization and its use in a serological survey. Rev Soc Bras Med Trop 38: 294300.[Crossref] [Google Scholar]
  27. Koizumi N, Muto M, Tanikawa T, Mizutani H, Sohmura Y, Hayashi E, Akao N, Hoshino M, Kawabata H, Watanabe H, , 2009. Human leptospirosis cases and the prevalence of rats harbouring Leptospira interrogans in urban areas of Tokyo, Japan. J Med Microbiol 58: 12271230.[Crossref] [Google Scholar]
  28. Kawabata H, Sakakibara S, Imai Y, Masuzawa T, Fujita H, Tsurumi M, Sato F, Takano A, Nogami S, Kaneda K, Watanabe H, , 2006. First record of Leptospira borgpetersenii isolation in the Amami Islands, Japan. Microbiol Immunol 50: 429434.[Crossref] [Google Scholar]
  29. Villanueva SY, Ezoe H, Baterna RA, Yanagihara Y, Muto M, Koizumi N, Fukui T, Okamoto Y, Masuzawa T, Cavinta LL, Gloriani NG, Yoshida S-i, , 2010. Serologic and molecular studies of Leptospira and leptospirosis among rats in the Philippines. Am J Trop Med Hyg 82: 889898.[Crossref] [Google Scholar]
  30. Scialfa E, Bolpe J, Bardón JC, Ridao G, Gentile J, Gallicchio O, , 2010. Isolation of Leptospira interrogans from suburban rats in Tandil, Buenos Aires, Argentina. Rev Argent Microbiol 42: 126128. [Google Scholar]
  31. Collares-Pereira M, Korver H, Terpstra WJ, Santos-Reis M, Ramalhinho MG, Mathias ML, Oom MM, Fons R, Libois R, Petrucci-Fonseca F, , 1997. First epidemiological data on pathogenic leptospires isolated on the Azorean islands. Eur J Epidemiol 13: 435441.[Crossref] [Google Scholar]
  32. Rahelinirina S, Léon A, Harstskeerl RA, Sertour N, Ahmed A, Raharimanana C, Ferquel E, Garnier M, Chartier L, Duplantier J-M, Rahalison L, Cornet M, , 2010. First isolation and direct evidence for the existence of large small-mammal reservoirs of Leptospira sp. in Madagascar. PLoS ONE 5: e14111.[Crossref] [Google Scholar]
  33. Thaipadungpanit J, Wuthiekanun V, Chierakul W, Smythe LD, Petkanchanapong W, Limpaiboon R, Apiwatanaporn A, Slack AT, Suputtamongkol Y, White NJ, Feil EJ, Day NP, Peacock SJ, , 2007. A dominant clone of Leptospira interrogans associated with an outbreak of human leptospirosis in Thailand. PLoS Negl Trop Dis 1: e56.[Crossref] [Google Scholar]
  34. de Faria MT, Calderwood MS, Athanazio DA, McBride AJ, Hartskeerl RA, Pereira MM, Ko AI, Reis MG, , 2008. Carriage of Leptospira interrogans among domestic rats from an urban setting highly endemic for leptospirosis in Brazil. Acta Trop 108: 15.[Crossref] [Google Scholar]
  35. Levett PN, Walton D, Waterman LD, Whittington CU, Mathison GE, Everard CO, , 1998. Surveillance of leptospiral carriage by feral rats in Barbados. West Indian Med J 47: 1517. [Google Scholar]
  36. Cheke A, , 2010. The timing of arrival of humans and their commensal animals on western Indian Ocean oceanic islands. Phelsuma 18: 3869. [Google Scholar]
  37. Cheke A, Hume J, , 2008. Lost Land of the Dodo. London: T&AD Poyser and A&C Black Publishers. [Google Scholar]
  38. Tollenaere C, Brouat C, Duplantier JM, Rahalison L, Rahelinirina S, Pascal M, Moné H, Mouahid G, Leirs H, Cosson JF, , 2010. Phylogeography of the introduced species Rattus rattus in the western Indian Ocean, with special emphasis on the colonization history of Madagascar. J Biogeogr 37: 398410.[Crossref] [Google Scholar]
  39. Dikken H, Timmer VE, Njenga R, , 1981. Three new leptospiral serovars from Kenya. Trop Geogr Med 33: 343346. [Google Scholar]
  40. Feresu SB, Steigerwalt AG, Brenner DJ, , 1999. DNA relatedness of Leptospira strains isolated from beef cattle in Zimbabwe. Int J Syst Bacteriol 49: 11111117.[Crossref] [Google Scholar]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2012.12-0102
Loading
/content/journals/10.4269/ajtmh.2012.12-0102
Loading

Data & Media loading...

  • Received : 14 Feb 2012
  • Accepted : 12 Apr 2012

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error