1921
Volume 88, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

We screened for malaria in 594 blood samples from febrile patients who tested negative by a -specific histidine-rich protein-2-based rapid diagnostic test at 12 health facilities in Zanzibar districts North A and Micheweni, from May to August 2010. Screening was with microscopy, polymerase chain reaction (PCR) targeting the gene (PCR) of the four major human malaria species, and quantitative PCR (qPCR). The prevalence of PCR-detectable malaria infection was 2% (12 of 594), including 8 , 3 and 1 infections. Microscopy identified 4 of 8 infections. Parasite density as estimated by microscopy or qPCR was > 4,000 parasites/μL in 5 of 8 PCR-detectable infections. The infections that were missed by the rapid diagnostic test represent a particular challenge in malaria elimination settings and highlight the need for more sensitive point-of-care diagnostic tools to improve case detection of all human malaria species in febrile patients.

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  • Received : 10 Feb 2012
  • Accepted : 01 Oct 2012

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