Volume 87, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



We describe sequences of six strains of dengue virus (DENV): three DENV-1 isolates and two DENV-4 isolates from Puerto Rico, and a DENV-1 strain from Key West, Florida, obtained from blood donors during 2010 epidemics. Phylogenetic analysis revealed that the Puerto Rico DENV-1 strains constitute a new lineage within genotype V different from those that circulated in Puerto Rico during the past two decades. The newer Puerto Rico DENV-1 strains associated with strains from the Caribbean and South America. The DENV-1 strain from Key West, Florida clustered with a strain isolated from mosquito pools collected in that area and with a number of strains from Nicaragua and Mexico circulating during 2006–2009. The Puerto Rico DENV-4 isolates of genotype II associated with strains that have circulated on the island throughout the 1980s and 1990s and with strains from the Caribbean region and Central America. Introduction and circulation of novel DENV lineages in dengue-endemic regions have the potential to increase the severity of dengue cases.


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  1. Guzman MG, Halstead SB, Artsob H, Buchy P, Farrar J, Gubler DJ, Hunsperger E, Kroeger A, Margolis HS, Martínez E, Nathan MB, Pelegrino JL, Simmons C, Yoksan S, Peeling RW, , 2010. Dengue: a continuing global threat. Nat Rev Microbiol 8: S7S16.[Crossref]
  2. Vasilakis N, Cardosa J, Hanley KA, Holmes EC, Weaver SC, , 2011. Fever from the forest: prospects for the continued emergence of sylvatic dengue virus and its impact on public health. Nat Rev Microbiol 9: 532541.[Crossref]
  3. Chen R, Vasilakis N, , 2011. Dengue - Quo tu et quo vadis? Viruses 3: 15621608.[Crossref]
  4. Rico-Hesse R, , 2007. Dengue virus evolution and virulence models. Clin Infect Dis 44: 14621466.[Crossref]
  5. Centers for Disease Control and Prevention, 2011. Largest Dengue Outbreak in Puerto Rico History. The Dengue Update. Vol. 3, No. 1. February 16, 2011. Available at: http://www.cdc.gov/dengue/dengue_upd/resources/DengueUpdateVo3No1.pdf. Accessed November 28, 2011.
  6. Centers for Disease Control and Prevention, 2007. Dengue hemorrhagic fever - U.S.–Mexico border, 2005. MMWR Morb Mortal Wkly Rep 56: 785789.
  7. Effler PV, Pang L, Kitsutani P, Vorndam V, Nakata M, Ayers T, Elm J, Tom T, Reiter P, Rigau-Perez JG, Hayes JM, Mills K, Napier M, Clark GG, Gubler DJ, Hawaii Dengue Outbreak Investigation Team, , 2005. Dengue fever, Hawaii, 2001–2002. Emerg Infect Dis 11: 742749.[Crossref]
  8. Gubler D, Gubler D, Kuno G, , 1997. Dengue and dengue hemorrhagic fever: its history and resurgence as a global public health problem. , eds. Dengue and Dengue Hemorrhagic Fever. New York, NY: CAB International, 175198.
  9. Centers for Disease Control and Prevention, 2010. Locally acquired dengue - Key West, Florida, 2009–2010. MMWR Morb Mortal Wkly Rep 59: 577581.
  10. Adalja AA, Sell TK, Bouri N, Franco C, , 2012. Lessons learned during dengue outbreaks in the United States, 2001–2011. Emerg Infect Dis 18: 608614.[Crossref]
  11. Johnson BW, Russell BJ, Lanciotti RS, , 2005. Serotype-specific detection of dengue viruses in a fourplex real-time reverse transcriptase PCR assay. J Clin Microbiol 43: 49774983.[Crossref]
  12. Resch W, Zaslavsky L, Kiryutin B, Rozanov M, Bao Y, Tatusova TA, , 2009. The virus variation resources at the National Center for Biotechnology Information: dengue virus. BMC Microbiol 9: 65.[Crossref]
  13. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S, , 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 27312719.[Crossref]
  14. Ronquist F, Huelsenbeck JP, , 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 15721574.[Crossref]
  15. Drummond AJ, Rambaut A, , 2007. BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evol Biol 7: 214.[Crossref]
  16. Goncalvez AP, Escalante AA, Pujol FH, Ludert JE, Tovar D, Salas RA, Liprandi F, , 2002. Diversity and evolution of the envelope gene of dengue virus type 1. Virology 303: 110119.[Crossref]
  17. Graham AS, Pruszynski CA, Hribar LJ, DeMay DJ, Tambasco AN, Hartley AE, Fussell EM, Michael SF, Isern S, , 2011. Mosquito-associated dengue virus, Key West, Florida, USA, 2010. Emerg Infect Dis 17: 20742075.[Crossref]
  18. Bennett SN, Holmes EC, Chirivella M, Rodriguez DM, Beltran M, Vorndam V, Gubler DJ, McMillan WO, , 2003. Selection-driven evolution of emergent dengue virus. Mol Biol Evol 20: 16501658.[Crossref]
  19. Bennett SN, Drummond AJ, Kapan DD, Suchard MA, Muñoz-Jordán JL, Pybus OG, Holmes EC, Gubler DJ, , 2010. Epidemic dynamics revealed in dengue evolution. Mol Biol Evol 27: 811818.[Crossref]
  20. McElroy KL, Santiago GA, Lennon NJ, Birren BW, Henn MR, Muñoz-Jordán JL, , 2011. Endurance, refuge, and reemergence of dengue virus type 2, Puerto Rico, 1986–2007. Emerg Infect Dis 17: 6471.[Crossref]
  21. OhAinle M, Balmaseda A, Macalalad AR, Tellez Y, Zody MC, Saborío S, Nuñez A, Lennon NJ, Birren BW, Gordon A, Henn MR, Harris E, , 2011. Dynamics of dengue disease severity determined by the interplay between viral genetics and serotype-specific immunity. Sci Transl Med 3: 114128.[Crossref]
  22. Añez G, Morales-Betoulle ME, Rios M, , 2011. Circulation of different lineages of dengue virus type 2 in Central America, their evolutionary time-scale and selection pressure analysis. PLoS ONE 6: e27459.[Crossref]

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  • Received : 09 Feb 2012
  • Accepted : 21 May 2012

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