1921
Volume 86, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Because malaria is still a significant problem worldwide, additional control methods need to be developed. The sporozoite is a good target for control measures because it displays dual infectivity for both mosquito and vertebrate host tissues. The gene, , was chosen as a candidate for study based on data from PlasmoDB, the database, indicating that it is expressed both at the transcriptional and protein levels in sporozoites, likely encodes a putative surface protein, and may have a potential role in the invasion of host tissues. Additional sequence analysis shows that the PFE0565w protein has orthologs in other species, but none outside of the genus . expresses transcript during both the sporozoite and erythrocytic stages of the parasite life cycle, where an alternative transcript was discovered during the erythrocytic stages. Data show that transcript is not present during axenic exoerythrocytic stages. Despite transcript being present in several life cycle stages, the PFE0565w protein is present only during the salivary gland sporozoite stage. Because the PFE0565w protein is present in salivary gland sporozoites, it could be a novel candidate for a pre-erythrocytic stage vaccine.

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2017-11-22
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References

  1. Porter WD, , 2006. Imported malaria and conflict: 50 years of experience in the U.S. Military. Mil Med 171: 925928.[Crossref]
  2. Crompton PD, Pierce SK, Miller LH, , 2010. Advances and challenges in malaria vaccine development. J Clin Invest 120: 41684178.[Crossref]
  3. Ejigiri I, Sinnis P, , 2009. Plasmodium sporozoite-host interactions from the dermis to the hepatocyte. Curr Opin Microbiol 12: 401407.[Crossref]
  4. Menard R, Sultan AA, Cortes C, Altszuler R, van Dijk MR, Janse CJ, Waters AP, Nussenzweig RS, Nussenzweig V, , 1997. Circumsporozoite protein is required for development of malaria sporozoites in mosquitoes. Nature 385: 336340.[Crossref]
  5. Sinnis P, Coppi A, Toida T, Toyoda H, Kinoshita-Toyoda A, Xie J, Kemp MM, Linhardt RJ, , 2007. Mosquito heparan sulfate and its potential role in malaria infection and transmission. J Biol Chem 282: 2537625384.[Crossref]
  6. Thathy V, Fujioka H, Gantt S, Nussenzweig R, Nussenzweig V, Menard R, , 2002. Levels of circumsporozoite protein in the Plasmodium oocyst determine sporozoite morphology. EMBO J 21: 15861596.[Crossref]
  7. Warburg A, Touray M, Krettli AU, Miller LH, , 1992. Plasmodium gallinaceum: antibodies to circumsporozoite protein prevent sporozoites from invading the salivary glands of Aedes aegypti . Exp Parasitol 75: 303307.[Crossref]
  8. Cohen J, Nussenzweig V, Nussenzweig R, Vekemans J, Leach A, , 2010. From the circumsporozoite protein to the RTS, S/AS candidate vaccine. Hum Vaccin 6: 9096.[Crossref]
  9. Bahl A, Brunk B, Coppel RL, Crabtree J, Diskin SJ, Fraunholz MJ, Grant GR, Gupta D, Huestis RL, Kissinger JC, Labo P, Li L, McWeeney SK, Milgram AJ, Roos DS, Schug J, Stoeckert CJ, Jr, 2002. PlasmoDB: the Plasmodium genome resource. An integrated database providing tools for accessing, analyzing and mapping expression and sequence data (both finished and unfinished). Nucleic Acids Res 30: 8790.[Crossref]
  10. Florens L, Washburn MP, Raine JD, Anthony RM, Grainger M, Haynes JD, Moch JK, Muster N, Sacci JB, Tabb DL, Witney AA, Wolters D, Wu Y, Gardner MJ, Holder AA, Sinden RE, Yates JR, Carucci DJ, , 2002. A proteomic view of the Plasmodium falciparum life cycle. Nature 419: 520526.[Crossref]
  11. Carter R, Ranford-Cartwright L, Alano P, , 1993. The culture and preparation of gametocytes of Plasmodium falciparum for immunochemical, molecular, and mosquito infectivity studies. Methods Mol Biol 21: 6788.
  12. Kappe S, Nussenzweig V, Kaiser K, Camargo N, Singh A, , 2010. Plasmodium axenic liver stages as a noninfectious whole organism malaria vaccine. Patent Application (Patentdocs ed.).
  13. Kissinger JC, Brunk BP, Crabtree J, Fraunholz MJ, Gajria B, Milgram AJ, Pearson DS, Schug J, Bahl A, Diskin SJ, Ginsburg J, Grant GR, Gupta D, Labo P, Li L, Mailman MD, McWeeney SK, Stoeckert CJ, Roos DS, , 2002. The Plasmodium genome database. Nature 419: 490492.[Crossref]
  14. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ, , 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 33893402.[Crossref]
  15. LaCrue AN, Sivaguru M, Walter MF, Fidock DA, James AA, Beerntsen BT, , 2006. A ubiquitous Plasmodium protein displays a unique surface labeling pattern in sporozoites. Mol Biochem Parasitol 148: 199209.[Crossref]
  16. Lacrue AN, James AA, Beerntsen BT, , 2005. The novel Plasmodium gallinaceum sporozoite protein, Pg93, is preferentially expressed in the nucleus of oocyst sporozoites. Am J Trop Med Hyg 73: 634643.
  17. Su XZ, Wu Y, Sifri CD, Wellems TE, , 1996. Reduced extension temperatures required for PCR amplification of extremely A+T-rich DNA. Nucleic Acids Res 24: 15741575.[Crossref]
  18. Blackman MJ, Whittle H, Holder AA, , 1991. Processing of the Plasmodium falciparum major merozoite surface protein-1: identification of a 33-kilodalton secondary processing product which is shed prior to erythrocyte invasion. Mol Biochem Parasitol 49: 3544.[Crossref]
  19. Rener J, Graves PM, Carter R, Williams JL, Burkot TR, , 1983. Target antigens of transmission-blocking immunity on gametes of Plasmodium falciparum . J Exp Med 158: 976981.[Crossref]
  20. Quakyi IA, Carter R, Rener J, Kumar N, Good MF, Miller LH, , 1987. The 230-kDa gamete surface protein of Plasmodium falciparum is also a target for transmission-blocking antibodies. J Immunol 139: 42134217.
  21. Klemba M, Beatty W, Gluzman I, Goldberg DE, , 2004. Trafficking of plasmepsin II to the food vacuole of the malaria parasite Plasmodium falciparum . J Cell Biol 164: 4756.[Crossref]
  22. Crabb BS, Rug M, Gilberger TW, Thompson JK, Triglia T, Maier AG, Cowman AF, , 2004. Transfection of the human malaria parasite Plasmodium falciparum . Methods Mol Biol 270: 263276.
  23. Adjalley SH, Lee MC, Fidock DA, , 2010. A method for rapid genetic integration into Plasmodium falciparum utilizing mycobacteriophage Bxb1 integrase. Methods Mol Biol 634: 87100.[Crossref]
  24. Talman AM, Blagborough AM, Sinden RE, , 2010. A Plasmodium falciparum strain expressing GFP throughout the parasite's life-cycle. PLoS ONE 5: e9156.[Crossref]
  25. Bahl A, Brunk B, Crabtree J, Fraunholz MJ, Gajria B, Grant GR, Ginsburg H, Gupta D, Kissinger JC, Labo P, Lil L, Mailman MD, Milgram AJ, Pearson DS, Roos DS, Schug J, Stoeckert CJ, Jr Whetzel P, , 2003. PlasmoDB: the Plasmodium genome resource. A database integrating experimental and computational data. Nucleic Acids Res 31: 212215.[Crossref]
  26. Bendtsen JD, Nielsen H, von Heijne G, Brunak S, , 2004. Improved prediction of signal peptides: SignalP 3.0. J Mol Biol 340: 783795.[Crossref]
  27. Emanuelsson O, Nielsen H, Brunak S, von Heijne G, , 2000. Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. J Mol Biol 300: 10051016.[Crossref]
  28. Nakai K, Horton P, , 1999. PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization. Trends Biochem Sci 24: 3436.[Crossref]
  29. Gattiker A, Gasteiger E, Bairoch A, , 2002. ScanProsite: a reference implementation of a PROSITE scanning tool. Appl Bioinformatics 1: 107108.
  30. Bozdech Z, Llinas M, Pulliam BL, Wong ED, Zhu J, DeRisi JL, , 2003. The transcriptome of the intraerythrocytic developmental cycle of Plasmodium falciparum . PLoS Biol 1: E5.[Crossref]
  31. Le Roch KG, Zhou Y, Blair PL, Grainger M, Moch JK, Haynes JD, De La Vega P, Holder AA, Batalov S, Carucci DJ, Winzeler EA, , 2003. Discovery of gene function by expression profiling of the malaria parasite life cycle. Science 301: 15031508.[Crossref]
  32. Young JA, Fivelman QL, Blair PL, de la Vega P, Le Roch KG, Zhou Y, Carucci DJ, Baker DA, Winzeler EA, , 2005. The Plasmodium falciparum sexual development transcriptome: a microarray analysis using ontology-based pattern identification. Mol Biochem Parasitol 143: 6779.[Crossref]
  33. Gunasekera AM, Patankar S, Schug J, Eisen G, Kissinger J, Roos D, Wirth DF, , 2004. Widespread distribution of antisense transcripts in the Plasmodium falciparum genome. Mol Biochem Parasitol 136: 3542.[Crossref]
  34. Hughes KR, Philip N, Lucas Starnes G, Taylor S, Waters AP, , 2010. From cradle to grave: RNA biology in malaria parasites. Wiley Interdiscip Rev RNA 1: 287303.[Crossref]
  35. Lu F, Jiang H, Ding J, Mu J, Valenzuela JG, Ribeiro JM, Su XZ, , 2007. cDNA sequences reveal considerable gene prediction inaccuracy in the Plasmodium falciparum genome. BMC Genomics 8: 255.[Crossref]
  36. Sorber K, Dimon MT, DeRisi JL, , 2011. RNA-Seq analysis of splicing in Plasmodium falciparum uncovers new splice junctions, alternative splicing and splicing of antisense transcripts. Nucleic Acids Res 39: 38203835.[Crossref]
  37. Hall N, Karras M, Raine JD, Carlton JM, Kooij TW, Berriman M, Florens L, Janssen CS, Pain A, Christophides GK, James K, Rutherford K, Harris B, Harris D, Churcher C, Quail MA, Ormond D, Doggett J, Trueman HE, Mendoza J, Bidwell SL, Rajandream MA, Carcucci DJ, Yates, JR, 3rd Kafatos FC, Janse CJ, Barrell B, Turner CM, Waters AP, Sinden RE, , 2005. A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses. Science 307: 8286.[Crossref]
  38. Khan SM, Franke-Fayard B, Mair GR, Lasonder E, Janse CJ, Mann M, Waters AP, , 2005. Proteome analysis of separated male and female gametocytes reveals novel sex-specific Plasmodium biology. Cell 121: 675687.[Crossref]
  39. Lasonder E, Ishihama Y, Andersen JS, Vermunt AM, Pain A, Sauerwein RW, Eling WM, Hall N, Waters AP, Stunnenberg HG, Mann M, , 2002. Analysis of the Plasmodium falciparum proteome by high-accuracy mass spectrometry. Nature 419: 537542.[Crossref]
  40. Siau A, Silvie O, Franetich JF, Yalaoui S, Marinach C, Hannoun L, van Gemert GJ, Luty AJ, Bischoff E, David PH, Snounou G, Vaquero C, Froissard P, Mazier D, , 2008. Temperature shift and host cell contact up-regulate sporozoite expression of Plasmodium falciparum genes involved in hepatocyte infection. PLoS Pathog 4: e1000121.[Crossref]
  41. Tarun AS, Peng X, Dumpit RF, Ogata Y, Silva-Rivera H, Camargo N, Daly TM, Bergman LW, Kappe SH, , 2008. A combined transcriptome and proteome survey of malaria parasite liver stages. Proc Natl Acad Sci USA 105: 305310.[Crossref]
  42. Williams CT, Azad AF, , 2010. Transcriptional analysis of the pre-erythrocytic stages of the rodent malaria parasite, Plasmodium yoelii . PLoS ONE 5: e10267.[Crossref]
  43. Fidock DA, Wellems TE, , 1997. Transformation with human dihydrofolate reductase renders malaria parasites insensitive to WR99210 but does not affect the intrinsic activity of proguanil. Proc Natl Acad Sci USA 94: 1093110936.[Crossref]
  44. Schuster FL, , 2002. Cultivation of Plasmodium spp. Clin Microbiol Rev 15: 355364.[Crossref]
  45. Trager W, Jensen JB, , 1997. Continuous culture of Plasmodium falciparum: its impact on malaria research. Int J Parasitol 27: 9891006.[Crossref]
  46. Westenberger SJ, McClean CM, Chattopadhyay R, Dharia NV, Carlton JM, Barnwell JW, Collins WE, Hoffman SL, Zhou Y, Vinetz JM, Winzeler EA, , 2010. A systems-based analysis of Plasmodium vivax lifecycle transcription from human to mosquito. PLoS Negl Trop Dis 4: e653.[Crossref]
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Supplementary Data

Supplementary PDF

  • Received : 22 Dec 2011
  • Accepted : 06 Feb 2012

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