Volume 87, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Leishmaniasis is an emerging disease in Thailand. Herein, we report on two human immunodeficiency virus (HIV)-infected patients with leishmaniasis who presented with overlapping manifestations between cutaneous and visceral leishmaniasis. Sequencing analysis of the internal transcribed spacer 1 (ITS1) of the ribosomal RNA gene showed that the species was identical to a new species recently described in Thailand. The detection of DNA of this species in saliva may have important implications for transmission and epidemiological studies.


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  1. Cupolillo E, Grimaldi G, Jr Momen H, , 1994. A general classification of New World Leishmania using numerical zymotaxonomy. Am J Trop Med Hyg 50: 296311.
  2. World Health Organization, 1990. Control of the Leishmaniasis. Technical Report Series 793. Geneva: WHO, 2125.
  3. Murray HW, Berman JD, Davies CR, Saravia NG, , 2005. Advances in leishmaniasis. Lancet 366: 15611577.[Crossref]
  4. Alvar J, Aparicio P, Aseffa A, Den Boer M, Cañavate C, Dedet JP, Gradoni L, Ter Horst R, López-Vélez R, Moreno J, , 2008. The relationship between leishmaniasis and AIDS: the second 10 years. Clin Microbiol Rev 21: 334359.[Crossref]
  5. Viriyavejakul P, Viravan C, Riganti M, Punpoowong B, , 1997. Imported cutaneous leishmaniasis in Thailand. Southeast Asian J Trop Med Public Health 28: 558562.
  6. Thisyakorn U, Jongwutiwes S, Vanichsetakul P, Lertsapcharoen P, , 1999. Visceral leishmaniasis: the first indigenous case report in Thailand. Trans R Soc Trop Med Hyg 93: 2324.[Crossref]
  7. Sukmee T, Siripattanapipong S, Mungthin M, Worapong J, Rangsin R, Samung Y, Kongkaew W, Bumrungsana K, Chanachai K, Apiwathanasorn C, Rujirojindakul P, Wattanasri S, Ungchusak K, Leelayoova S, , 2008. A suspected new species of Leishmania, the causative agent of visceral leishmaniasis in a Thai patient. Int J Parasitol 38: 617622.[Crossref]
  8. Suankratay C, Suwanpimolkul G, Wilde H, Siriyasatien P, , 2010. Case report: autochthonous visceral leishmaniasis in a human immunodeficiency virus (HIV)-infected patient: the first in Thailand and review of the literature. Am J Trop Med Hyg 82: 48.[Crossref]
  9. Maharom P, Siripattanapipong S, Mungthin M, Naaglor T, Sukkawee R, Pudkorn R, Wattana W, Wanachiwanawin D, Areechokchai D, Leelayoova S, , 2008. Visceral leishmaniasis caused by Leishmania infantum in Thailand. Southeast Asian J Trop Med Public Health 39: 988990.
  10. Bureau of Epidemiology, Department of Disease Control, Ministry of Public Health, Thailand, 2010. Annual reports of opportunistic infection in thai HIV infected-patient (Thai). Available at: http://www.boe.moph.go.th/files/report/20110401_19956923.pdf. Accessed November 1, 2011.
  11. Ridley DS, Ridley MJ, , 1983. The evolution of the lesion in cutaneous leishmaniasis. J Pathol 141: 8396.[Crossref]
  12. Weigle KA, Davalos MD, Heredia P, , 1987. Diagnosis of cutaneous and mucocutaneous leishmaniasis in Colombia: a comparison of seven methods. Am J Trop Med Hyg 36: 489496.
  13. Albrecht H, Sobottka I, Emminger C, , 1996. Visceral leishmaniasis emerging as an important opportunistic infection in HIV-infected persons living in areas nonendemic for Leishmania donovani . Arch Pathol Lab Med 120: 189198.
  14. Hofman V, Marty P, Perrin C, , 2000. The histological spectrum of visceral leishmaniasis caused by Leishmania infantum MON-1 in acquired immune deficiency syndrome. Hum Pathol 31: 7584.[Crossref]
  15. Mimori T, Sasaki J, Nakata M, Gomez EA, Uezato H, Nonaka S, , 1998. Rapid identification of Leishmania species from formalin-fixed biopsy samples by polymorphism-specific polymerase chain reaction. Gene 210: 179186.[Crossref]
  16. Pirmez C, da Silva Trajano V, Paes-Oliveira Neto MP, da-Cruz AM, Gonçalves-da-Costa SC, Catanho M, , 1999. Use of PCR in diagnosis of human American tegumentary leishmaniasis in Rio de Janeiro, Brazil. J Clin Microbiol 37: 18191823.
  17. Forkner CE, Zia LS, , 1934. Viable Leishmania donovani in nasal and oral secretions of patients with kala-azar and the bearing of this finding on the transmission of the disease. J Exp Med 59: 491499.[Crossref]
  18. Romero GA, Sampaio RN, Macedo V de O, Marsden PD, , 1999. Sensitivity of a vacuum aspiratory culture technique for diagnosis of localized cutaneous leishmaniasis in an endemic area of Leishmania (Viannia) braziliensis . Mem Inst Oswaldo Cruz 94: 505508.[Crossref]
  19. Galaï Y, Chabchoub N, Ben-Abid M, Ben-Abda I, Ben-Alaya-Bouafif N, Amri F, Aoun K, Bouratbine A, , 2011. Diagnosis of Mediterranean visceral leishmaniasis by detection of Leishmania antibodies and Leishmania DNA in oral fluid samples collected using an Oracol device. J Clin Microbiol 49: 31503153.[Crossref]
  20. Spanakos G, Piperaki ET, Menounos PG, Tegos N, Flemetakis A, Vakalis NC, , 2008. Detection and species identification of Old World Leishmania in clinical samples using a PCR-based method. Trans R Soc Trop Med Hyg 102: 4653.[Crossref]
  21. Magill AJ, Grogl M, Johnson SC, Gasser RA, Jr, 1994. Visceral infection due to Leishmania tropica in a veteran of operation Desert Storm who presented 2 years after leaving Saudi Arabia. Clin Infect Dis 19: 805806.[Crossref]
  22. Guerin PJ, Olliaro P, Sundar S, Boelaert M, Croft SL, Desjeux P, , 2002. Visceral leishmaniasis: current status of control, diagnosis, and treatment, and a proposed research and development agenda. Lancet Infect Dis 2: 494501.[Crossref]
  23. Rodriguez-Bonfante C, Bonfante-Garrido R, Grimaldi G, Jr Momen H, Cupolillo E, , 2003. Genotypically distinct Leishmania colombiensis isolates from Venezuela cause both cutaneous and visceral leishmaniasis in humans. Infect Genet Evol 3: 119124.[Crossref]
  24. Dowlati Y, , 1996. Cutaneous leishmaniasis: clinical aspects. Clin Dermatol 14: 425431.[Crossref]
  25. Weigle K, Saravia NG, , 1996. Natural history, clinical evaluation, and the host-parasite interaction in New World cutaneous leishmaniasis. Clin Dermatol 14: 433450.[Crossref]
  26. Marovich M, Lira R, Shepard M, Fuchs G, Kruetzer R, Nutmann T, Neva F, , 2001. Leishmaniasis recidivans recurrence after 43 years: a clinical and immunologic report after successful treatment. Clin Infect Dis 33: 10761079.[Crossref]
  27. Mebrahtu YB, Hendricks LD, Oster CN, Lawyer PG, Perkins PV, Pamba H, , 1993. Leishmania donovani parasites in the nasal secretions, tonsillopharyngeal mucosa, and urine centrifugates of visceral leishmaniasis patients in Kenya. Am J Trop Med Hyg 48: 530535.
  28. Shorft HE, Smith ROA, D'Silva HAH, Swaminath CS, , 1923. Leishmania donovani in human faeces in Indian kala-azar. Indian J Med Res 17: 644646.
  29. Shortt HE, Swaminath CS, , 1937. The viability of Leishmania donovani excreted in the nasal mucus in Indian kala-azar. Indian J Med Res 25: 341343.
  30. Corvalan FH, Sampaio RNR, Brustoloni YM, Andreotti R, Lima MSC, Jr, 2011. DNA identification of Leishmania (Viannia) braziliensis in human saliva from a patient with American cutaneous leishmaniasis. J Venom Anim Toxins Trop Dis 17: 98102.[Crossref]

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  • Received : 02 Dec 2011
  • Accepted : 17 Apr 2012

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