Volume 87, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



In May of 2010, two communities (Truenococha and Santa Marta) reported to be at risk of vampire bat depredation were surveyed in the Province Datem del Marañón in the Loreto Department of Perú. Risk factors for bat exposure included age less than or equal to 25 years and owning animals that had been bitten by bats. Rabies virus neutralizing antibodies (rVNAs) were detected in 11% (7 of 63) of human sera tested. Rabies virus ribonucleoprotein (RNP) immunoglobulin G (IgG) antibodies were detected in the sera of three individuals, two of whom were also seropositive for rVNA. Rabies virus RNP IgM antibodies were detected in one respondent with no evidence of rVNA or RNP IgG antibodies. Because one respondent with positive rVNA results reported prior vaccination and 86% (six of seven) of rVNA-positive respondents reported being bitten by bats, these data suggest nonfatal exposure of persons to rabies virus, which is likely associated with vampire bat depredation.

[open-access] This is an Open Access article distributed under the terms of the American Society of Tropical Medicine and Hygiene's Re-use License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


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  1. Knobel DL, Cleaveland S, Coleman PG, Fevre EM, Meltzer MI, Miranda ME, Shaw A, Zinsstag J, Meslin FX, , 2005. Re-evaluating the burden of rabies in Africa and Asia. Bull World Health Organ 83: 360368.
  2. Rupprecht CE, Hanlon CA, Hemachudha T, , 2002. Rabies re-examined. Lancet Infect Dis 2: 327343.[Crossref]
  3. Messenger SL, Smith JS, Rupprecht CE, , 2002. Emerging epidemiology of bat-associated cryptic cases of rabies in humans in the United States. Clin Infect Dis 35: 738747.[Crossref]
  4. Salmon-Mulanovich G, Vasquez A, Albujar C, Guevara C, Laguna-Torres VA, Salazar M, Zamalloa H, Caceres M, Gomez-Benavides J, Pacheco V, Contreras C, Kochel T, Niezgoda M, Jackson FR, Velasco-Villa A, Rupprecht C, Montgomery JM, , 2009. Human rabies and rabies in vampire and nonvampire bat species, southeastern Peru, 2007. Emerg Infect Dis 15: 13081310.[Crossref]
  5. Oliveira RD, de Souza SP, Lobo RSV, Castilho JG, Macedo CI, Carnieli P, Fahl WO, Achkar SM, Scheffer KC, Kotait I, Carrieri ML, Brandao PE, , 2010. Rabies virus in insectivorous bats: implications of the diversity of the nucleoprotein and glycoprotein genes for molecular epidemiology. Virology 405: 352360.[Crossref]
  6. Diaz AM, Papo S, Rodriguez A, Smith JS, , 1994. Antigenic analysis of rabies-virus isolates from Latin America and the Caribbean. Zoonoses Public Health 41: 153160.
  7. de Mattos CA, Favi M, Yung V, Pavletic C, de Mattos CC, , 2000. Bat rabies in urban centers in Chile. J Wildl Dis 36: 231240.[Crossref]
  8. Favoretto SR, Carrieri ML, Cunha EM, Aguiar EA, Silva LH, Sodre MM, Souza MC, Kotait I, , 2002. Antigenic typing of Brazilian rabies virus samples isolated from animals and humans, 1989–2000. Rev Inst Med Trop Sao Paulo 44: 9195.[Crossref]
  9. Arellano-Sota C, , 1988. Vampire bat-transmitted rabies in cattle. Rev Infect Dis 10 (Suppl 4): S707S709.[Crossref]
  10. Carneiro AJ, Franke CR, Stocker A, Dos Santos F, Ungar de Sa JE, Moraes-Silva E, Alves JN, Brunink S, Corman VM, Drosten C, Drexler JF, , 2010. Rabies virus RNA in naturally infected vampire bats, northeastern Brazil. Emerg Infect Dis 16: 20042006.[Crossref]
  11. Velasco-Villa A, Orciari LA, Juarez-Islas V, Gomez-Sierra M, Padilla-Medina I, Flisser A, Souza V, Castillo A, Franka R, Escalante-Mane M, Sauri-Gonzalez I, Rupprecht CE, , 2006. Molecular diversity of rabies viruses associated with bats in Mexico and other countries of the Americas. J Clin Microbiol 44: 16971710.[Crossref]
  12. Schneider MC, Romijn PC, Uieda W, Tamayo H, da Silva DF, Belotto A, da Silva JB, Leanes LF, , 2009. Rabies transmitted by vampire bats to humans: an emerging zoonotic disease in Latin America? Rev Panam Salud Publica 25: 260269.[Crossref]
  13. Warner CK, Zaki SR, Shieh WJ, Whitfield SG, Smith JS, Orciari LA, Shaddock JH, Niezgoda M, Wright CW, Goldsmith CS, Sanderlin DW, Yager PA, Rupprecht CE, , 1999. Laboratory investigation of human deaths from vampire bat rabies in Peru. Am J Trop Med Hyg 60: 502507.
  14. Lopez A, Miranda P, Tejada E, Fishbein DB, , 1992. Outbreak of human rabies in the Peruvian jungle. Lancet 339: 408411.[Crossref]
  15. Gómez-Benavides J, , 2011. Veintiocho de Setiembre, día Mundial de Prevención de la Rabia. Bol Epidemiol Lima: Dirección General de Epidemiología. Lima, Peru: Ministerio de Salud, 825826.
  16. Canahuiri J, Vargas E, , 2009. Caso Confirmado de Rabia Humana en la Comunidad Indígena Amazónica de Quebrada Kandungos, Distrito de Río Santiago, Provincia de Condorcanqui. DIRESA Amazonas. Bol. Epidemiol. Lima, Peru: Ministerio de Salud, 276.
  17. Canahuiri J, , 2009. Rabia Silvestre Humana en la Comunidad Indígena Amazónica del Centro Poblado Los Angeles, Quiteni, Distrito Río Tambo, Provincia Satipo, Junín, 2009. Bol Epidemiol. Lima, Peru: Ministerio de Salud, 805.
  18. Canahuiri J, , 2009. Probable Brote de Rabia Transmitida por Vampiro en la Comunidad Nativa (CC.NN) Cachiaco, Distrito Nieva, Provincia Condorcanqui, DIRESA Amazonas. Bol Epidemiol. Lima, Peru: Ministerio de Salud, 839.
  19. Dirección General de Epidemiología, 2010. Brote de Rabia Humana Silvestre en Comunidades Nativas Kigkis y Sumpa del Distrito Nieva, Provincia Condorcanqui, DIRESA Amazonas. Bol Epidemiol. Lima, Peru: Ministerio de Salud, 137138.
  20. Dirección General de Epidemiología, 2011. Brote de Rabia Silvestre en la Comunidad Nativa San Ramón- Yupicusa, Distrito Imaza, Provincia Bagua, Amazonas – 2011: Situación Actual. Bol Epidemiol. Lima, Peru: Ministerio de Salud, 123124.
  21. CDC, 2008. Human rabies prevention—United States, 2008: recommendations of the Advisory Committee on Immunization Practices. MMWR Recomm Rep 57: 128.
  22. Blancou J, , 1988. Ecology and epidemiology of fox rabies. Rev Infect Dis 10 (Suppl 4): S606S609.[Crossref]
  23. Hanlon CA, Niezgoda M, Rupprecht C, Jackson A, Wunner W, , 2007. Rabies in terrestrial animals. , eds. Rabies, 2nd Ed. London: Academic Press, 201258.[Crossref]
  24. Trimarchi C, Debbie JG, , 1977. Naturally occurring rabies virus and neutralizing antibody in two species of insectivorous bats of New York state. J Wildl Dis 13: 366369.[Crossref]
  25. Lord RD, Fuenzalida E, Delpietro H, Larghi OP, de Diaz AM, Lazaro L, , 1975. Observations on the epizootiology of vampire bat rabies. Bull Pan Am Health Organ 9: 189195.
  26. Turmelle AS, Allen LC, Jackson FR, Kunz TH, Rupprecht C, McCracken GF, , 2010. Ecology of rabies virus exposure in colonies of Brazilian free-tailed bats (Tadarida brasiliensis) at natural and man-made roosts in Texas. Vector Borne Zoonotic Dis 10: 165175.[Crossref]
  27. Burns KF, Shelton DF, Grogan EW, , 1958. Bat rabies: experimental host transmission studies. Ann N Y Acad Sci 70: 452466.[Crossref]
  28. Allworth A, Murray K, Morgan J, , 1996. A human case of encephalitis due to a lyssavirus recently identified in fruit bats. Commun Dis Intell 20: 504.
  29. Paweska JT, Blumberg LH, Liebenberg C, Hewlett RH, Grobbelaar AA, Leman PA, Croft JE, Nel LH, Nutt L, Swanepoel R, , 2006. Fatal human infection with rabies-related Duvenhage virus, South Africa. Emerg Infect Dis 12: 19651967.[Crossref]
  30. Lumio J, Hillbom M, Roine R, Ketonen L, Haltia M, Valle M, Neuvonen E, Lahdevirta J, , 1986. Human rabies of bat origin in Europe. Lancet 1: 378.[Crossref]
  31. Bell JF, , 1964. Abortive rabies infection. I. Experimental production in white mice and general discussion. J Infect Dis 114: 249257.[Crossref]
  32. Hattwick MA, Weis TT, Stechschulte CJ, Baer GM, Gregg MB, , 1972. Recovery from rabies. A case report. Ann Intern Med 76: 931942.[Crossref]
  33. Willoughby RE, Jr Tieves KS, Hoffman GM, Ghanayem NS, Amlie-Lefond CM, Schwabe MJ, Chusid MJ, Rupprecht CE, , 2005. Survival after treatment of rabies with induction of coma. N Engl J Med 352: 25082514.[Crossref]
  34. Porras C, Barboza JJ, Fuenzalida E, Adaros HL, Oviedo AM, Furst J, , 1976. Recovery from rabies in man. Ann Intern Med 85: 4448.[Crossref]
  35. Alvarez L, Fajardo R, Lopez E, Pedroza R, Hemachudha T, Kamolvarin N, Cortes G, Baer GM, , 1994. Partial recovery from rabies in a nine-year-old boy. Pediatr Infect Dis J 13: 11541155.[Crossref]
  36. CDC, 1977. Rabies in a laboratory worker—New York. MMWR Morb Mortal Wkly Rep 26: 183184.
  37. Madhusudana SN, Nagaraj D, Uday M, Ratnavalli E, Kumar MV, , 2002. Partial recovery from rabies in a six-year-old girl. Int J Infect Dis 6: 8586.[Crossref]
  38. CDC, 2010. Presumptive abortive human rabies—Texas, 2009. MMWR Morb Mortal Wkly Rep 59: 185190.
  39. Smith JS, Yager P, Baer G, Meslin FX, Kaplan MM, Koprowski H, , 1996. A rapid fluorescent focus inhibition test (RFFIT) for determining rabies virus-neutralizing antibody. , eds. Laboratory Techniques in Rabies. Geneva: World Health Organization, 181192.
  40. Reed LJ, Muench H, , 1938. A simple method of estimating fifty percent endpoints. Am J Hyg 27: 493497.
  41. Noah DL, Drenzek CL, Smith JS, Krebs JW, Orciari L, Shaddock J, Sanderlin D, Whitfield S, Fekadu M, Olson JG, Rupprecht CE, Childs JE, , 1998. Epidemiology of human rabies in the United States, 1980 to 1996. Ann Intern Med 128: 922930.[Crossref]
  42. Black D, Wiktor TJ, , 1986. Survey of raccoon hunters for rabies antibody titers: pilot study. J Fla Med Assoc 73: 517520.
  43. Orr PH, Rubin MR, Aoki FY, , 1988. Naturally acquired serum rabies neutralizing antibody in a Canadian Inuit population. Arctic Med Res 47 (Suppl 1): 699700.
  44. Follmann EH, Ritter DG, Beller M, , 1994. Survey of fox trappers in northern Alaska for rabies antibody. Epidemiol Infect 113: 137141.[Crossref]
  45. Moore SM, Gordon CR, Briggs D, Jackson A, Wunner W, , 2007. Rabies serology. , eds. Rabies, 2nd Ed. London: Academic Press, 471488.[Crossref]
  46. Lodmell DL, , 1983. Genetic control of resistance to street rabies virus in mice. J Exp Med 157: 451460.[Crossref]
  47. Lodmell DL, Chesebro B, , 1984. Murine resistance to street rabies virus: genetic analysis by testing second-backcross progeny and verification of allelic resistance genes in SJL/J and CBA/J mice. J Virol 50: 359362.
  48. Templeton JW, Holmberg C, Garber T, Sharp RM, , 1986. Genetic control of serum neutralizing-antibody response to rabies vaccination and survival after a rabies challenge infection in mice. J Virol 59: 98102.
  49. Rodriguez-Delfin LA, Rubin-de-Celis VE, Zago MA, , 2001. Genetic diversity in an Andean population from Peru and regional migration patterns of Amerindians in South America: data from Y chromosome and mitochondrial DNA. Hum Hered 51: 97106.[Crossref]
  50. Callegari-Jacques SM, Salzano FM, Weimer WA, Hutz MH, Black EL, Santos SEB, Guerreiro JF, Mestrineri MM, Pandey JP, , 1994. Further blood genetic studies on Amazonian diversity—data from four Indian groups. Ann Hum Biol 21: 465481.[Crossref]
  51. Batista Dos Santos SE, Rodrigues JD, Ribeiro Dos Santos AKC, Zago MA, , 1999. Differential contribution of indigenous men and women to the formation of an urban population in the Amazon region as revealed by mtDNA and Y-DNA. Am J Phys Anthropol 109: 175180.[Crossref]
  52. Moore SM, Wilkerson MJ, Davis RD, Wyatt CR, Briggs DJ, , 2006. Detection of cellular immunity to rabies antigens in human vaccinees. J Clin Immunol 26: 533545.[Crossref]
  53. Hooper DC, Morimoto K, Bette M, Weihe E, Koprowski H, Dietzschold B, , 1998. Collaboration of antibody and inflammation in clearance of rabies virus from the central nervous system. J Virol 72: 37113719.
  54. Dietzschold B, Wang HH, Rupprecht CE, Celis E, Tollis M, Ertl H, Heber-Katz E, Koprowski H, , 1987. Induction of protective immunity against rabies by immunization with rabies virus ribonucleoprotein. Proc Natl Acad Sci USA 84: 91659169.[Crossref]
  55. Turner GS, Nicholson KG, Tyrrell DA, Aoki FY, , 1982. Evaluation of a human diploid cell strain rabies vaccine: final report of a three year study of pre-exposure immunization. J Hyg (Lond) 89: 101110.[Crossref]
  56. Turmelle AS, Jackson FR, Green D, McCracken GF, Rupprecht CE, , 2010. Host immunity to repeated rabies virus infection in big brown bats. J Gen Virol 91: 23602366.[Crossref]
  57. Crepin P, Audry L, Rotivel Y, Gacoin A, Caroff C, Bourhy H, , 1998. Intravitam diagnosis of human rabies by PCR using saliva and cerebrospinal fluid. J Clin Microbiol 36: 11171121.
  58. Anderson LJ, Nicholson KG, Tauxe RV, Winkler WG, , 1984. Human rabies in the United States, 1960 to 1979: epidemiology, diagnosis, and prevention. Ann Intern Med 100: 728735.[Crossref]
  59. Kasempimolporn S, Hemachudha T, Khawplod P, Manatsathit S, , 1991. Human immune response to rabies nucleocapsid and glycoprotein antigens. Clin Exp Immunol 84: 195199.[Crossref]
  60. Wang ZW, Sarmento L, Wang Y, Li XQ, Dhingra V, Tseggai T, Jiang B, Fu ZF, , 2005. Attenuated rabies virus activates, while pathogenic rabies virus evades, the host innate immune responses in the central nervous system. J Virol 79: 1255412565.[Crossref]
  61. Morimoto K, Hooper DC, Spitsin S, Koprowski H, Dietzschold B, , 1999. Pathogenicity of different rabies virus variants inversely correlates with apoptosis and rabies virus glycoprotein expression in infected primary neuron cultures. J Virol 73: 510518.
  62. Faber M, Pulmanausahakul R, Hodawadekar SS, Spitsin S, McGettigan JP, Schnell MJ, Dietzschold B, , 2002. Overexpression of the rabies virus glycoprotein results in enhancement of apoptosis and antiviral immune response. J Virol 76: 33743381.[Crossref]
  63. Morimoto K, Patel M, Corisdeo S, Hooper DC, Fu ZF, Rupprecht CE, Koprowski H, Dietzschold B, , 1996. Characterization of a unique variant of bat rabies virus responsible for newly emerging human cases in North America. Proc Natl Acad Sci USA 93: 56535658.[Crossref]
  64. Wiktor TJ, Dietzschold B, Leamnson RN, Koprowski H, , 1977. Induction and biological properties of defective interfering particles of rabies virus. J Virol 21: 626635.
  65. Briggs DJ, Jackson A, Wunner W, , 2007. Human rabies vaccines. , eds. Rabies, 2nd Ed. London: Academic Press, 505515.[Crossref]
  66. Briggs DJ, Schwenke JR, , 1992. Longevity of rabies antibody titre in recipients of human diploid cell rabies vaccine. Vaccine 10: 125129.[Crossref]
  67. Fuenzalida E, , 1972. Human pre-exposure rabies immunization with suckling mouse brain vaccine. Bull World Health Organ 46: 561563.
  68. Perrin P, Versmisse P, Delagneau JF, Lucas G, Rollin PE, Sureau P, , 1986. The influence of the type of immunosorbent on rabies antibody EIA; advantages of purified glycoprotein over whole virus. J Biol Stand 14: 95102.[Crossref]
  69. Lee TK, Hutchinson HD, Ziegler DW, , 1977. Comparison of rabies humoral antibody titers in rabbits and humans by indirect radioimmunoassay, rapid-fluorescent-focus-inhibition technique, and indirect fluorescent-antibody assay. J Clin Microbiol 5: 320325.
  70. Gómez-Benavides J, Laguna-Torres VA, Recuenco S, , 2010. The real significance of being bitten by a hematophagous bat in indigenous communities in the remote Peruvian Amazon. Rev Peru Med Exp Salud Publica 27: 649658.[Crossref]
  71. Greenhall AM, Joermann G, Schmidt U, Seidel MR, , 1983. Desmodus rotundus. Mammalian Species 202: 16.[Crossref]
  72. Greenhall AM, Greenhall AM, Schmidt U, , 1988. Feeding behavior. , eds. Natural History of Vampire Bats. Boca Raton, FL: CRC Press, 111131.
  73. Constantine DG, Greenhall AM, Schmidt U, , 1988. Transmission of pathogenic microorganisms by vampire bats. , eds. Natural History of Vampire Bats. Boca Raton: CRC Press, Inc., 167189.
  74. de Verteuil E, Urich FW, , 1936. The study and control of paralytic rabies transmitted by vampire bats in Trinidad, B.W.I. Trans R Soc Trop Med Hyg 29: 317347.[Crossref]
  75. Goncalves MA, Sa-Neto RJ, Brazil TK, , 2002. Outbreak of aggressions and transmission of rabies in human beings by vampire bats in northeastern Brazil. Rev Soc Bras Med Trop 35: 461464.[Crossref]
  76. Prieto JF, Baer GM, , 1972. An outbreak of bovine paralytic rabies in Tuxtepec, Oaxaca, Mexico. Am J Trop Med Hyg 21: 219225.
  77. McCarthy TJ, , 1989. Human depredation by vampire bats (Desmodus rotundus) following a hog cholera campaign. Am J Trop Med Hyg 40: 320322.
  78. Rupprecht CE, Turmelle A, Kuzmin IV, , 2011. A perspective on lyssavirus emergence and perpetuation. Curr Opin Virol 1: 662670.[Crossref]
  79. Badrane H, Bahloul C, Perrin P, Tordo N, , 2001. Evidence of two Lyssavirus phylogroups with distinct pathogenicity and immunogenicity. J Virol 75: 32683276.[Crossref]
  80. Markotter W, Kuzmin IV, Rupprecht CE, Nel LH, , 2009. Lagos bat virus virulence in mice inoculated by the peripheral route. Epidemiol Infect 137: 11551162.[Crossref]
  81. Hughes GJ, Kuzmin IV, Schmitz A, Blanton J, Manangan J, Murphy S, Rupprecht CE, , 2006. Experimental infection of big brown bats (Eptesicus fuscus) with Eurasian bat lyssaviruses Aravan, Khujand, and Irkut virus. Arch Virol 151: 20212035.[Crossref]
  82. Franka R, Johnson N, Muller T, Vos A, Neubert L, Freuling C, Rupprecht CE, Fooks AR, , 2008. Susceptibility of North American big brown bats (Eptesicus fuscus) to infection with European bat lyssavirus type 1. J Gen Virol 89: 19982010.[Crossref]
  83. Johnson N, Vos A, Neubert L, Freuling C, Mansfield KL, Kaipf I, Denzinger A, Hicks D, Nunez A, Franka R, Rupprecht CE, Muller T, Fooks AR, , 2008. Experimental study of European bat lyssavirus type-2 infection in Daubenton's bats (Myotis daubentonii). J Gen Virol 89: 26622672.[Crossref]
  84. Banyard AC, Hayman D, Johnson N, McElhinney L, Fooks AR, , 2011. Bats and lyssaviruses. Adv Virus Res 79: 239289.[Crossref]
  85. Dodet B, Africa Rabies Bureau (AfroREB), 2009. The fight against rabies in Africa: from recognition to action. Vaccine 27: 50275032.[Crossref]
  86. Favoretto SR, de Mattos CC, de Morais NB, Carrieri ML, Rolim BN, Silva LM, Rupprecht CE, Durigon EL, de Mattos CA, , 2006. Rabies virus maintained by dogs in humans and terrestrial wildlife, Ceara State, Brazil. Emerg Infect Dis 12: 19781981.[Crossref]
  87. Favi M, de Mattos CA, Yung V, Chala E, Lopez LR, de Mattos CC, , 2002. First case of human rabies in chile caused by an insectivorous bat virus variant. Emerg Infect Dis 8: 7981.[Crossref]

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  • Received : 03 Nov 2011
  • Accepted : 13 Apr 2012

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