1921
Volume 86, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

We conducted contact tracing and high-risk group screening using pooled real-time polymerase chain reaction (PCR) to support malaria elimination in Thailand. PCR detected more infections than the local and expert microscopists. High-throughput pooling technique reduced costs and allowed prompt reporting of results.

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2012-05-01
2017-11-18
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References

  1. The malERA Consultative Group on Diagnoses and Diagnostics, 2011. A research agenda for malaria eradication: diagnoses and diagnostics. PloS Med 8: e1000396.[Crossref]
  2. Moonen B, Cohen JM, Snow RW, Slutsker L, Drakeley C, Smith DL, Abeyasinghe RR, Rodrigues MH, Maharaj R, Tanner M, Targett G, , 2010. Operational strategies to achieve and maintain malaria elimination. Lancet 376: 15921603.[Crossref]
  3. Perkins M, Bell D, , 2008. Working without a blindfold: the critical role of diagnostics in malaria control. Malar J 7: S5.[Crossref]
  4. Ouédraogo AL, Bousema T, Schneider P, de Vlas SJ, Ilboudo-Sanogo E, Cuzin-Ouattara N, Nébié I, Roeffen W, Berhave JP, Luty AFJ, Sauerwein R, , 2009. Substantial contribution of submicroscopical Plasmodium falciparum gametocyte carriage to the infectious reservoir in an area of seasonal transmission. PLoS One 4: e8410.[Crossref]
  5. Steenkeste N, Rogers W, Okell L, Jeanne I, Incardona S, Duval L, Chy S, Hewitt S, Chou M, Socheat D, Babin F-X, Ariey F, Rogier C, , 2010. Sub-microscopic malaria cases and mixed malaria infection in a remote area of high malaria endemicity in Rattanakiri province, Cambodia: implication for malaria elimination. Malar J 9: 108.[Crossref]
  6. Harris I, Sharrock W, Bain L, Gray K-A, Bobogare A, Boaz L, Lilley K, Krause D, Vallely A, Johnson M-L, Gatton M, Shanks G, Cheng Q, , 2010. A large proportion of asymptomatic Plasmodium infections with low and sub-microscopic parasite densities in the low transmission setting of Temotu Province, Solomon Islands: challenges for malaria diagnostics in an elimination setting. Malar J 9: 254.[Crossref]
  7. Taylor SM, Juliano JJ, Trottman PA, Griffin JB, Landis SH, Kitsa P, Tshefu AK, Meshnick SR, , 2010. High-throughput pooling and real-time PCR-based strategy for malaria detection. J Clin Microbiol 48: 512519.[Crossref]
  8. Hsiang MS, Lin M, Dokomajilar C, Kemere J, Pilcher CD, Dorsey G, Greenhouse B, , 2010. PCR-based pooling of dried blood spots for detection of malaria parasites: optimization and application to a cohort of Ugandan children. J Clin Microbiol 48: 35393543.[Crossref]
  9. Plowe CV, Djimde A, Bouare M, Doumbo O, Wellems TE, , 1995. Pyrimethamine and proguanil resistance-conferring mutations in Plasmodium falciparum dihydrofolate reductase: polymerase chain reaction methods for surveillance in Africa. Am J Trop Med Hyg 52: 565568.
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  • Received : 05 Oct 2011
  • Accepted : 15 Feb 2012

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